Chemical diversity and biological activity of secondary metabolites in eight species of Curcuma

Curcuma (Zingiberacae) are documented to originate from Indo-Malayan region and known to be a rich source of secondary metabolites with interesting pharmaceutical properties. There are at least eight species of this genus known to be utilized in the Malay community. However, not much information...

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Main Author:	Ummul Mardiah Elias
Format:	Thesis
Language:	English
Published:	2015
Subjects:	QK Botany
Online Access:	https://eprints.ums.edu.my/id/eprint/18957/1/Chemical%20diversity%20and%20biological.pdf
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spelling	my-ums-ep.189572018-02-28T06:46:47Z Chemical diversity and biological activity of secondary metabolites in eight species of Curcuma 2015 Ummul Mardiah Elias QK Botany Curcuma (Zingiberacae) are documented to originate from Indo-Malayan region and known to be a rich source of secondary metabolites with interesting pharmaceutical properties. There are at least eight species of this genus known to be utilized in the Malay community. However, not much information is available pertaining to its chemical composition nor of its biological potential. In this investigation, the volatile essential oil and its secondary metabolites were extracted, isolated and determined via chromatographic means. The essential oils were obtained by hydrodistillation and analyzed using GCMS. There were 49 compounds in C. aeruginosa (0.17%), 46 compounds in C. caesia (0.16%), 65 compounds in C. heyneana (0.23%), 25 compounds in C. longa (0.33%), 10 compounds in C. mangga (0.19%), 15 compounds in C. rotunda (0.16%), 22 compounds in C. xanthorrhiza (0.36%) and 20 compounds in C. zedoaria (0.31%), which then be categorize into four group. Belonging to group one, C. aeruginosa, C. caesia, C. longa and C. xanthorrhiza, contains high composition of oxygenated sesquiterpenes while in group (2), C rotunda and C. zedoaria have high content of oxygenated monoterpenes. By having high content of hydrogenated sesquiterpenes and hydrogenated monoterpenes., C. heyneana and C. mangga were placed in group 3 and 4, respectively. On the other hand, rhizome extract derived via soxhlet and analyzed through PTLC and NMR, revealed presence of 8 compounds in C aeruginosa (5.81%) and C caesia (7.56%), 7 compounds in C. heyneana (3.32%), 6 compounds in C longa (3.11%), 2 compounds in C mangga (1.92%), 5 compounds in C. rotunda (2.07%), 6 compounds in C. xanthorrhiza (3.23%) and 6 compounds in C. zedoaria (3.72%). Analysis on type of compouds showed that C. aeruginosa and C. caesia belong to group 1 (germacrane, carabrane, guaiane and elemene), C zedoaria in group 2 (germacrane and elemene), C heyneana, C mangga, C longa, and C xanthorrhiza in group 3 (diphenylheptanoid) and C rotunda in group 4 (monoterpenoids). In addition, cude extract and essential oil of Curcuma sp. (1mg/ml) were evaluated for their biocidal activity againts food-borne bacteria. Essential oil of C. xanthorrhiza showed good inhibition zone againts Vibrio parahaemolyticus Furthermore, via MIC and MBA testing, essential oil of Curcuma exhibit ; i) bakteriostatic agent-C mangga, C rotunda and C zedoaria, ii) Bactericidal agent- C heyneana, C longa and C xanthorrhiza against Staphylococcus aureus. While, essential oil of Curcuma exhibit; i) Bakteriostatic agent-C heyneana, C mangga, C rotunda and C zedoaria, ii) Bactericidal agent -C longa dan C. xanthorrhiza againts MRSA. Moreover, it was also found moderate anticancer activity from compounds isolated from Curcuma sp.; compound 9 and 10 against MCF-7 cell, and compound 7,10,11 and 12 against HeLa cell lines. 2015 Thesis https://eprints.ums.edu.my/id/eprint/18957/ https://eprints.ums.edu.my/id/eprint/18957/1/Chemical%20diversity%20and%20biological.pdf text en public masters Universiti Malaysia Sabah Institute for Tropical Biology and Conservation
institution	Universiti Malaysia Sabah
collection	UMS Institutional Repository
language	English
topic	QK Botany
spellingShingle	QK Botany Ummul Mardiah Elias Chemical diversity and biological activity of secondary metabolites in eight species of Curcuma
description	Curcuma (Zingiberacae) are documented to originate from Indo-Malayan region and known to be a rich source of secondary metabolites with interesting pharmaceutical properties. There are at least eight species of this genus known to be utilized in the Malay community. However, not much information is available pertaining to its chemical composition nor of its biological potential. In this investigation, the volatile essential oil and its secondary metabolites were extracted, isolated and determined via chromatographic means. The essential oils were obtained by hydrodistillation and analyzed using GCMS. There were 49 compounds in C. aeruginosa (0.17%), 46 compounds in C. caesia (0.16%), 65 compounds in C. heyneana (0.23%), 25 compounds in C. longa (0.33%), 10 compounds in C. mangga (0.19%), 15 compounds in C. rotunda (0.16%), 22 compounds in C. xanthorrhiza (0.36%) and 20 compounds in C. zedoaria (0.31%), which then be categorize into four group. Belonging to group one, C. aeruginosa, C. caesia, C. longa and C. xanthorrhiza, contains high composition of oxygenated sesquiterpenes while in group (2), C rotunda and C. zedoaria have high content of oxygenated monoterpenes. By having high content of hydrogenated sesquiterpenes and hydrogenated monoterpenes., C. heyneana and C. mangga were placed in group 3 and 4, respectively. On the other hand, rhizome extract derived via soxhlet and analyzed through PTLC and NMR, revealed presence of 8 compounds in C aeruginosa (5.81%) and C caesia (7.56%), 7 compounds in C. heyneana (3.32%), 6 compounds in C longa (3.11%), 2 compounds in C mangga (1.92%), 5 compounds in C. rotunda (2.07%), 6 compounds in C. xanthorrhiza (3.23%) and 6 compounds in C. zedoaria (3.72%). Analysis on type of compouds showed that C. aeruginosa and C. caesia belong to group 1 (germacrane, carabrane, guaiane and elemene), C zedoaria in group 2 (germacrane and elemene), C heyneana, C mangga, C longa, and C xanthorrhiza in group 3 (diphenylheptanoid) and C rotunda in group 4 (monoterpenoids). In addition, cude extract and essential oil of Curcuma sp. (1mg/ml) were evaluated for their biocidal activity againts food-borne bacteria. Essential oil of C. xanthorrhiza showed good inhibition zone againts Vibrio parahaemolyticus Furthermore, via MIC and MBA testing, essential oil of Curcuma exhibit ; i) bakteriostatic agent-C mangga, C rotunda and C zedoaria, ii) Bactericidal agent- C heyneana, C longa and C xanthorrhiza against Staphylococcus aureus. While, essential oil of Curcuma exhibit; i) Bakteriostatic agent-C heyneana, C mangga, C rotunda and C zedoaria, ii) Bactericidal agent -C longa dan C. xanthorrhiza againts MRSA. Moreover, it was also found moderate anticancer activity from compounds isolated from Curcuma sp.; compound 9 and 10 against MCF-7 cell, and compound 7,10,11 and 12 against HeLa cell lines.
format	Thesis
qualification_level	Master's degree
author	Ummul Mardiah Elias
author_facet	Ummul Mardiah Elias
author_sort	Ummul Mardiah Elias
title	Chemical diversity and biological activity of secondary metabolites in eight species of Curcuma
title_short	Chemical diversity and biological activity of secondary metabolites in eight species of Curcuma
title_full	Chemical diversity and biological activity of secondary metabolites in eight species of Curcuma
title_fullStr	Chemical diversity and biological activity of secondary metabolites in eight species of Curcuma
title_full_unstemmed	Chemical diversity and biological activity of secondary metabolites in eight species of Curcuma
title_sort	chemical diversity and biological activity of secondary metabolites in eight species of curcuma
granting_institution	Universiti Malaysia Sabah
granting_department	Institute for Tropical Biology and Conservation
publishDate	2015
url	https://eprints.ums.edu.my/id/eprint/18957/1/Chemical%20diversity%20and%20biological.pdf
_version_	1747836502259269632

Chemical diversity and biological activity of secondary metabolites in eight species of Curcuma

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