Antioxidative potential of selected edible wild mushrooms from Sabah
The objectives of the present study were to evaluate the in vitro antioxidant properties of four species of edible wild mushrooms namely, Pleurotus porrigens, Schizophyllum commune, Hygrocybe conica, and Lentinus ciliatus, optimization of the extraction conditions for antioxidant activity of the...
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| Format: | Thesis |
| Language: | English |
| Published: |
2013
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| Subjects: | |
| Online Access: | https://eprints.ums.edu.my/id/eprint/19724/1/Antioxidative%20potential%20of%20selected%20edible.pdf |
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| Summary: | The objectives of the present study were to evaluate the in vitro antioxidant
properties of four species of edible wild mushrooms namely, Pleurotus porrigens,
Schizophyllum commune, Hygrocybe conica, and Lentinus ciliatus, optimization of
the extraction conditions for antioxidant activity of the mushrooms; identification of
the potent antioxidative components from the selected mushrooms; and the
evaluation of the oxidative stability of cooking oil supplemented with selected
mushroom extracts. The antioxidant properties of edible wild mushrooms were
evaluated using 2,2-diphenyl-1-picrylhydrazyl (OPPH) radical scavenging ability,
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation inhibition,
ferric reducing antioxidant power (FRAP), and ß-carotene-linoleate bleaching (ß-CB).
Total phenolic content (TPC) was determined using Folin-Ciocalteu's method.
Response surface methodology (RSM) was employed to optimize the extraction
time and temperature of P. porrigens and S. commune for the maximal yields of
antioxidant activity. Fractionation by means of column chromatography was carried
out and the identification of major anti oxidative components was performed by
liquid chromatography-mass spectrometry analysis. The oxidative stability of
sunflower oil was determined by peroxide value, iodine value, p-anisidine value,
and thiobarbituric acid-reactive substances. Results showed that P. porrigens had
significantly higher (p < 0.05) DPPH radical scavenging ability and FRAP than that
of butylated hydroxyanisole (BHA), while S. commune showed comparable DPPH
radical scavenging ability and ABTS radical cation inhibition with BHA. Total
phenolic content was found in a descending order of P. porrigens > L. Ciliatus = P.
ostreatus > H. conica = S. commune. Positive correlations were found between
TPC and DPPH; ABTS; FRAP; and ß-CB, respectively, indicating that the presence of
phenolic compounds contribute to the antioxidant activities of the edible wild
mushrooms. The optimized extraction time and temperature of P. porrigens and S.
commune were 315.5 min and 37.4°C; and 213.2 min and 41.5°C, respectively.
The values obtained experimentally agreed well with the predicted values,
indicating the suitability of respective RSM models for maximal yields of antioxidant
activity. Both sub-fraction (SF)-III of P. porrigens and S. commune showed
consistently higher DPPH radical scavenging ability, FRAP, and TPC with two
flavones glucosides, namely luteolin 7-O-ß-glucoside and apigenin 7-O-ß-glucoside
were identified in both SF-III. The sunflower oils supplemented with P. porrigens
and S. commune extracts were found to be able to prolong the shelf-life between 1
and 2 years by retarding the formations of primary and secondary oxidation
products, and reducing losses of polyunsaturated fatty acids. In conclusion, the
selected edible wild mushrooms showed promising antioxidant activity, and luteolin
7-O-ß-glucoside and apigenin 7-O-ß-glucoside were identified in SF-III of P.
porrigens and S. commune. Thus, edible wild mushrooms from Sabah can be
promoted as antioxidant-rich foods as well as potential sources of natural
antioxidants for food industry application. |
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