Chemical and antioxidant potential of commercial and wild mushroom extracts as feed additive for Asian Sea Bass (Lates Calcarifer) Juveniles

Extracts of two edible mushrooms, Pleurotus sajor caju (commercial) and Schizophyllum commune (wild) were used to study the chemical compositions and the potency to use as feed additive on the immune response in Asian seabass, Lates calcarifer juveniles. Four types of solvents namely aqueous, ethano...

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Main Author:	Chong, Vivian Ai Ping
Format:	Thesis
Language:	English
Published:	2017
Subjects:	QK Botany
Online Access:	https://eprints.ums.edu.my/id/eprint/26499/1/Chemical%20and%20antioxidant%20potential%20of%20commercial%20and%20wild%20mushroom%20extracts%20as%20feed%20additive%20for%20Asian%20Sea%20Bass%20%28Lates%20Calcarifer%29%20Juveniles.pdf
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spelling	my-ums-ep.264992020-12-17T08:36:35Z Chemical and antioxidant potential of commercial and wild mushroom extracts as feed additive for Asian Sea Bass (Lates Calcarifer) Juveniles 2017 Chong, Vivian Ai Ping QK Botany Extracts of two edible mushrooms, Pleurotus sajor caju (commercial) and Schizophyllum commune (wild) were used to study the chemical compositions and the potency to use as feed additive on the immune response in Asian seabass, Lates calcarifer juveniles. Four types of solvents namely aqueous, ethanol, methanol and acetone were used in mushrooms extraction process to assess total polysaccharide, crude lipid, protein, amino acids, fatty acids, antioxidant activity and antimicrobial activity in mushrooms crude extracts. In feeding trial, one percent of aqueous P. sajor caju extract added diet (D1), one percent of aqueous S. commune extract added diet (D2) and commercial diet without mushroom extract (D0) were fed to L. calcarifer juveniles for 30 days. After 30 days, challenge assay was conducted by injecting each fish intraperitoneally with 108cfu/ml of V. harveyi bacterium suspension. Immune response was investigated by measuring antibody titre from blood serum and the survival rate (%) was observed for 10 days post-challenge. Total polysaccharide, crude lipid and protein composition were ranged from 2.07% to 22.08%, 0.01% to 0.32%, 5.07% to 17.14% and 13.34% to 25.48%, 0.10% to 0.65%, 6.25% to 10.70% in P. sajor caju and S. commune crude extract, respectively. P. sajor caju contained 19.04% to 20.34% of total AA and S. commune contained 20.29% to 31.62%. Aqueous extract has the highest amount of total EAA (8.95 ± 0.02%) in both mushrooms. DPPH scavenging activity obtained the highest antioxidant activity in 5mg/ml of S. commune and P. sajor caju extracts. P. sajor caju and S. commune aqueous extracts at 2mg/ml have shown the highest reducing power capability at 2.69 and 3.35 (700nm), respectively. The lowest EC50 (mg/ml) of in-vitro antioxidant assays were obtained in P. sajor caju (DPPH=1.47; reducing power=0.09) and S. commune (DPPH=1.52; reducing power=0.41) with significance of p <0.05 in aqueous extracts. Minimum inhibition concentration (MIC) values of V. harveyi, V. parahaemolyticus and V. anguillarum were <1.25mg/ml, <1.25mg/ml, 10mg/ml and <1.25mg/ml, 2.5mg/ml, 5mg/ml obtained in P. sajor caju and S. commune extracts, respectively. Fish fed in D1 and D2 have shown significance difference (p<0.05) in antibody titre compared to antibody titre obtained in D0 (control diet). D2 also demonstrated the highest survival of 90%, while fish fed with D1 and D0 recorded 55% and 60% of survival, respectively. The extract of S. commune has shown better chemical composition with aqueous extraction process. The highest survival of 90% with the development of antibody titre were also observed in S. commune extract inclusion diet. Extract of S. commune is the choice of option to boost immune system in Asian seabass juveniles. 2017 Thesis https://eprints.ums.edu.my/id/eprint/26499/ https://eprints.ums.edu.my/id/eprint/26499/1/Chemical%20and%20antioxidant%20potential%20of%20commercial%20and%20wild%20mushroom%20extracts%20as%20feed%20additive%20for%20Asian%20Sea%20Bass%20%28Lates%20Calcarifer%29%20Juveniles.pdf text en validuser mphil masters Universiti Malaysia Sabah Borneo Marine Research Institute
institution	Universiti Malaysia Sabah
collection	UMS Institutional Repository
language	English
topic	QK Botany
spellingShingle	QK Botany Chong, Vivian Ai Ping Chemical and antioxidant potential of commercial and wild mushroom extracts as feed additive for Asian Sea Bass (Lates Calcarifer) Juveniles
description	Extracts of two edible mushrooms, Pleurotus sajor caju (commercial) and Schizophyllum commune (wild) were used to study the chemical compositions and the potency to use as feed additive on the immune response in Asian seabass, Lates calcarifer juveniles. Four types of solvents namely aqueous, ethanol, methanol and acetone were used in mushrooms extraction process to assess total polysaccharide, crude lipid, protein, amino acids, fatty acids, antioxidant activity and antimicrobial activity in mushrooms crude extracts. In feeding trial, one percent of aqueous P. sajor caju extract added diet (D1), one percent of aqueous S. commune extract added diet (D2) and commercial diet without mushroom extract (D0) were fed to L. calcarifer juveniles for 30 days. After 30 days, challenge assay was conducted by injecting each fish intraperitoneally with 108cfu/ml of V. harveyi bacterium suspension. Immune response was investigated by measuring antibody titre from blood serum and the survival rate (%) was observed for 10 days post-challenge. Total polysaccharide, crude lipid and protein composition were ranged from 2.07% to 22.08%, 0.01% to 0.32%, 5.07% to 17.14% and 13.34% to 25.48%, 0.10% to 0.65%, 6.25% to 10.70% in P. sajor caju and S. commune crude extract, respectively. P. sajor caju contained 19.04% to 20.34% of total AA and S. commune contained 20.29% to 31.62%. Aqueous extract has the highest amount of total EAA (8.95 ± 0.02%) in both mushrooms. DPPH scavenging activity obtained the highest antioxidant activity in 5mg/ml of S. commune and P. sajor caju extracts. P. sajor caju and S. commune aqueous extracts at 2mg/ml have shown the highest reducing power capability at 2.69 and 3.35 (700nm), respectively. The lowest EC50 (mg/ml) of in-vitro antioxidant assays were obtained in P. sajor caju (DPPH=1.47; reducing power=0.09) and S. commune (DPPH=1.52; reducing power=0.41) with significance of p <0.05 in aqueous extracts. Minimum inhibition concentration (MIC) values of V. harveyi, V. parahaemolyticus and V. anguillarum were <1.25mg/ml, <1.25mg/ml, 10mg/ml and <1.25mg/ml, 2.5mg/ml, 5mg/ml obtained in P. sajor caju and S. commune extracts, respectively. Fish fed in D1 and D2 have shown significance difference (p<0.05) in antibody titre compared to antibody titre obtained in D0 (control diet). D2 also demonstrated the highest survival of 90%, while fish fed with D1 and D0 recorded 55% and 60% of survival, respectively. The extract of S. commune has shown better chemical composition with aqueous extraction process. The highest survival of 90% with the development of antibody titre were also observed in S. commune extract inclusion diet. Extract of S. commune is the choice of option to boost immune system in Asian seabass juveniles.
format	Thesis
qualification_name	Master of Philosophy (M.Phil.)
qualification_level	Master's degree
author	Chong, Vivian Ai Ping
author_facet	Chong, Vivian Ai Ping
author_sort	Chong, Vivian Ai Ping
title	Chemical and antioxidant potential of commercial and wild mushroom extracts as feed additive for Asian Sea Bass (Lates Calcarifer) Juveniles
title_short	Chemical and antioxidant potential of commercial and wild mushroom extracts as feed additive for Asian Sea Bass (Lates Calcarifer) Juveniles
title_full	Chemical and antioxidant potential of commercial and wild mushroom extracts as feed additive for Asian Sea Bass (Lates Calcarifer) Juveniles
title_fullStr	Chemical and antioxidant potential of commercial and wild mushroom extracts as feed additive for Asian Sea Bass (Lates Calcarifer) Juveniles
title_full_unstemmed	Chemical and antioxidant potential of commercial and wild mushroom extracts as feed additive for Asian Sea Bass (Lates Calcarifer) Juveniles
title_sort	chemical and antioxidant potential of commercial and wild mushroom extracts as feed additive for asian sea bass (lates calcarifer) juveniles
granting_institution	Universiti Malaysia Sabah
granting_department	Borneo Marine Research Institute
publishDate	2017
url	https://eprints.ums.edu.my/id/eprint/26499/1/Chemical%20and%20antioxidant%20potential%20of%20commercial%20and%20wild%20mushroom%20extracts%20as%20feed%20additive%20for%20Asian%20Sea%20Bass%20%28Lates%20Calcarifer%29%20Juveniles.pdf
_version_	1747836557486718976

Chemical and antioxidant potential of commercial and wild mushroom extracts as feed additive for Asian Sea Bass (Lates Calcarifer) Juveniles

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