Antioxidant properties of Carica Papaya leaves extracts prepared under different conditions and infused with “Kelulut” honey

Carica papaya is a tropical plant belonging to the Caricaceae family. Rich in phytochemical content, its leaves especially is renowned for its many health benefits especially for its anticancer properties as well as a supplement in treating dengue disease. This study thus aimed to optimise aqueous...

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Main Author:	Monjia Belleza Cosmas Mojulat
Format:	Thesis
Language:	English
Published:	2018
Subjects:	SF Animal culture
Online Access:	https://eprints.ums.edu.my/id/eprint/26572/1/Antioxidant%20properties%20of%20Carica%20Papaya%20leaves%20extracts%20prepared%20under%20different%20conditions%20and%20infused%20with%20%E2%80%9CKelulut%E2%80%9D%20honey.pdf
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spelling	my-ums-ep.265722021-05-27T03:22:14Z Antioxidant properties of Carica Papaya leaves extracts prepared under different conditions and infused with “Kelulut” honey 2018 Monjia Belleza Cosmas Mojulat SF Animal culture Carica papaya is a tropical plant belonging to the Caricaceae family. Rich in phytochemical content, its leaves especially is renowned for its many health benefits especially for its anticancer properties as well as a supplement in treating dengue disease. This study thus aimed to optimise aqueous extraction and to determine the effect of honey infusion on the leaves extract which was analysed using TPC, TFC, FRAP and DPPH assays respectively. Based on analysis of 1 g of sample extracted with 100ml deionised water, the optimal extraction conditions for aqueous extraction were determined to be at 700C for 20 minutes where its TPC was 9.97+0.47 mg GAE/mL (p<0.05), TFC was totaled at 2.64+0.01 mg QUE/mL (p<0.05) while its FRAP assay was amounted to 16.84+1.10 mg TE/mL. Radical scavenging values using DPPH assay was recorded to be 87.53% with its IC50 at 492.54+2.45 mg TE/mL (p<0.05). Study on the infusion of “kelulut” honey with the leaves extract provides evidence that not only does it improve the taste of the bitter papaya extract; the positive synergy also increases the overall antioxidant activity. The aforementioned tests and assays recorded higher antioxidant activity with increasing honey dosage (max 4tbsp.), where its TPC was 21.66+0.54 mg GAE/mL (p<0.05), while antioxidant activity based on FRAP assay yielded 24.02+0.87 mg TE/mL (p<0.05). Accordingly, radical scavenging activity based on DPPH assay was 98.20% and lower IC50 value compared to infusion at lower dosage, amounting at 408.02+4.98 mg TE/mL. Subsequently, gallic acid and quercetin isolated from freeze dried Carica papaya leaves extract was higher compared to freshly prepared sample. However, conversely, gallic acid and quercetin yielded from fresh “kelulut” honey and Carica papaya leaves extract infused with “kelulut” honey is higher in fresh sample, suggesting that preservation effect using freeze dry method differ depending on sample type and method of preparation. Future study can therefore be done to improve and develop standardised preparation of infused papaya leaves with honey, and subsequently releasing it for commercial usage. Universiti Malaysia Sabah 2018 Thesis https://eprints.ums.edu.my/id/eprint/26572/ https://eprints.ums.edu.my/id/eprint/26572/1/Antioxidant%20properties%20of%20Carica%20Papaya%20leaves%20extracts%20prepared%20under%20different%20conditions%20and%20infused%20with%20%E2%80%9CKelulut%E2%80%9D%20honey.pdf text en validuser Universiti Malaysia Sabah Faculty Of Science And Natural Resources
institution	Universiti Malaysia Sabah
collection	UMS Institutional Repository
language	English
topic	SF Animal culture
spellingShingle	SF Animal culture Monjia Belleza Cosmas Mojulat Antioxidant properties of Carica Papaya leaves extracts prepared under different conditions and infused with “Kelulut” honey
description	Carica papaya is a tropical plant belonging to the Caricaceae family. Rich in phytochemical content, its leaves especially is renowned for its many health benefits especially for its anticancer properties as well as a supplement in treating dengue disease. This study thus aimed to optimise aqueous extraction and to determine the effect of honey infusion on the leaves extract which was analysed using TPC, TFC, FRAP and DPPH assays respectively. Based on analysis of 1 g of sample extracted with 100ml deionised water, the optimal extraction conditions for aqueous extraction were determined to be at 700C for 20 minutes where its TPC was 9.97+0.47 mg GAE/mL (p<0.05), TFC was totaled at 2.64+0.01 mg QUE/mL (p<0.05) while its FRAP assay was amounted to 16.84+1.10 mg TE/mL. Radical scavenging values using DPPH assay was recorded to be 87.53% with its IC50 at 492.54+2.45 mg TE/mL (p<0.05). Study on the infusion of “kelulut” honey with the leaves extract provides evidence that not only does it improve the taste of the bitter papaya extract; the positive synergy also increases the overall antioxidant activity. The aforementioned tests and assays recorded higher antioxidant activity with increasing honey dosage (max 4tbsp.), where its TPC was 21.66+0.54 mg GAE/mL (p<0.05), while antioxidant activity based on FRAP assay yielded 24.02+0.87 mg TE/mL (p<0.05). Accordingly, radical scavenging activity based on DPPH assay was 98.20% and lower IC50 value compared to infusion at lower dosage, amounting at 408.02+4.98 mg TE/mL. Subsequently, gallic acid and quercetin isolated from freeze dried Carica papaya leaves extract was higher compared to freshly prepared sample. However, conversely, gallic acid and quercetin yielded from fresh “kelulut” honey and Carica papaya leaves extract infused with “kelulut” honey is higher in fresh sample, suggesting that preservation effect using freeze dry method differ depending on sample type and method of preparation. Future study can therefore be done to improve and develop standardised preparation of infused papaya leaves with honey, and subsequently releasing it for commercial usage.
format	Thesis
author	Monjia Belleza Cosmas Mojulat
author_facet	Monjia Belleza Cosmas Mojulat
author_sort	Monjia Belleza Cosmas Mojulat
title	Antioxidant properties of Carica Papaya leaves extracts prepared under different conditions and infused with “Kelulut” honey
title_short	Antioxidant properties of Carica Papaya leaves extracts prepared under different conditions and infused with “Kelulut” honey
title_full	Antioxidant properties of Carica Papaya leaves extracts prepared under different conditions and infused with “Kelulut” honey
title_fullStr	Antioxidant properties of Carica Papaya leaves extracts prepared under different conditions and infused with “Kelulut” honey
title_full_unstemmed	Antioxidant properties of Carica Papaya leaves extracts prepared under different conditions and infused with “Kelulut” honey
title_sort	antioxidant properties of carica papaya leaves extracts prepared under different conditions and infused with “kelulut” honey
granting_institution	Universiti Malaysia Sabah
granting_department	Faculty Of Science And Natural Resources
publishDate	2018
url	https://eprints.ums.edu.my/id/eprint/26572/1/Antioxidant%20properties%20of%20Carica%20Papaya%20leaves%20extracts%20prepared%20under%20different%20conditions%20and%20infused%20with%20%E2%80%9CKelulut%E2%80%9D%20honey.pdf
_version_	1747836558967308288

Antioxidant properties of Carica Papaya leaves extracts prepared under different conditions and infused with “Kelulut” honey

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