Determination of Gluconasturtiin and Glucotropaeolin contents and antioxidant properties of in vitro Grown Watercress (Nasturtium Officinale)

Watercress (Nasturtium officinale), a green vegetable belongs to the Brassicaceae, contains considerable amounts of vitamins, minerals and secondary metabolites such as glucosinolates (GS). Watercress contain phenyl ethyl glucosinolate (gluconasturtiin), i.e. precursor of phenyl ethyl isothiocyanate...

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Main Author:	Eramartica Rubin
Format:	Thesis
Language:	English
Published:	2018
Subjects:	SB Plant culture
Online Access:	https://eprints.ums.edu.my/id/eprint/26925/1/Determination%20of%20Gluconasturtiin%20and%20Glucotropaeolin%20contents%20and%20antioxidant%20properties%20of%20in%20vitro%20%20Grown%20Watercress.pdf
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spelling	my-ums-ep.269252021-06-02T03:16:12Z Determination of Gluconasturtiin and Glucotropaeolin contents and antioxidant properties of in vitro Grown Watercress (Nasturtium Officinale) 2018 Eramartica Rubin SB Plant culture Watercress (Nasturtium officinale), a green vegetable belongs to the Brassicaceae, contains considerable amounts of vitamins, minerals and secondary metabolites such as glucosinolates (GS). Watercress contain phenyl ethyl glucosinolate (gluconasturtiin), i.e. precursor of phenyl ethyl isothiocyanate (PEITC), which is widely reported to restrain the growth of cancer cells. The content of secondary metabolites and other compounds in plants is affected by different growth conditions such as pH, temperature, light intensity and nutrient supply. Thus, the aim of the current study is to evaluate the concentration of gluconasturtiin (and other GS) and antioxidant properties in watercress cultivated in vitro under different growth conditions. The samples were collected from watercress growing wild in a spring in Kundasang area, Ranau Sabah and subjected to sterilisation to establish N. officinale in vitro culture in the lab. The sterilization was done by using Chlorox® solution (5% v/v) containing Tween 20 to obtain 90% survival rate of the plants. It was grown inside glass jar containing hormone-free Murashige and Skoog (1962) and 0.25 % (v/v) of Plant Preservative Mixture (PPM) with and without elicitor/ natural additives. It was placed inside growth chamber at 25°C with 16 hours photoperiod and was harvested at 10 days interval after transplant until 60 days. Phenyl ethyl glucosinolate (PEGSL) and Benzyl glucosinolate (BGSL) increased over five-fold and three-fold, respectively, in non-treated plant culture compared to wild plant. Shoot culture of watercress had the ability to synthesize higher amounts of PEGSL (1.81 ± 0.04 µmol g-1FW) and BGSL (1.19 ± 0.03 µmol g-1 FW) when it is grown under non-elicited and without treatment of additives after thirty days. Amongst those plants samples treated with elicitors/additives, in vitro plant culture treated with casein hydrolysate shows the highest accumulation of PEGSL (1.60 ± 0.08 µmol/g FW) and BGSL (0.82 µmol/g). Elicited plant in this study has low glucosinolates content as compared to non-elicited plant. Nevertheless, all in vitro grown plant in this study showed higher concentration of PEGSL and BGSL compared to the matured wild plant. Plant grown without treatment (elicitors/ additives) shows highest FRAP value (77.05 ± 6.43 mg trolox/g DW), lowest IC50 (509.16 ± 7.03 µg/ml DW), highest total phenolic content (131 ± 1.49 GAE µg/ml) and highest flavonoid content (82.4 ± 7.04 µg/ml QU) as compared to wild plant and other in vitro watercress sample. Thus, in vitro culture watercress without elicitors (chitosan) and additives (casein hydrolysate, coconut water) is the richest source of glucosinolates and antioxidant in this study. 2018 Thesis https://eprints.ums.edu.my/id/eprint/26925/ https://eprints.ums.edu.my/id/eprint/26925/1/Determination%20of%20Gluconasturtiin%20and%20Glucotropaeolin%20contents%20and%20antioxidant%20properties%20of%20in%20vitro%20%20Grown%20Watercress.pdf text en validuser mphil masters Universiti Malaysia Sabah Faculty Of Science And Natural Resources
institution	Universiti Malaysia Sabah
collection	UMS Institutional Repository
language	English
topic	SB Plant culture
spellingShingle	SB Plant culture Eramartica Rubin Determination of Gluconasturtiin and Glucotropaeolin contents and antioxidant properties of in vitro Grown Watercress (Nasturtium Officinale)
description	Watercress (Nasturtium officinale), a green vegetable belongs to the Brassicaceae, contains considerable amounts of vitamins, minerals and secondary metabolites such as glucosinolates (GS). Watercress contain phenyl ethyl glucosinolate (gluconasturtiin), i.e. precursor of phenyl ethyl isothiocyanate (PEITC), which is widely reported to restrain the growth of cancer cells. The content of secondary metabolites and other compounds in plants is affected by different growth conditions such as pH, temperature, light intensity and nutrient supply. Thus, the aim of the current study is to evaluate the concentration of gluconasturtiin (and other GS) and antioxidant properties in watercress cultivated in vitro under different growth conditions. The samples were collected from watercress growing wild in a spring in Kundasang area, Ranau Sabah and subjected to sterilisation to establish N. officinale in vitro culture in the lab. The sterilization was done by using Chlorox® solution (5% v/v) containing Tween 20 to obtain 90% survival rate of the plants. It was grown inside glass jar containing hormone-free Murashige and Skoog (1962) and 0.25 % (v/v) of Plant Preservative Mixture (PPM) with and without elicitor/ natural additives. It was placed inside growth chamber at 25°C with 16 hours photoperiod and was harvested at 10 days interval after transplant until 60 days. Phenyl ethyl glucosinolate (PEGSL) and Benzyl glucosinolate (BGSL) increased over five-fold and three-fold, respectively, in non-treated plant culture compared to wild plant. Shoot culture of watercress had the ability to synthesize higher amounts of PEGSL (1.81 ± 0.04 µmol g-1FW) and BGSL (1.19 ± 0.03 µmol g-1 FW) when it is grown under non-elicited and without treatment of additives after thirty days. Amongst those plants samples treated with elicitors/additives, in vitro plant culture treated with casein hydrolysate shows the highest accumulation of PEGSL (1.60 ± 0.08 µmol/g FW) and BGSL (0.82 µmol/g). Elicited plant in this study has low glucosinolates content as compared to non-elicited plant. Nevertheless, all in vitro grown plant in this study showed higher concentration of PEGSL and BGSL compared to the matured wild plant. Plant grown without treatment (elicitors/ additives) shows highest FRAP value (77.05 ± 6.43 mg trolox/g DW), lowest IC50 (509.16 ± 7.03 µg/ml DW), highest total phenolic content (131 ± 1.49 GAE µg/ml) and highest flavonoid content (82.4 ± 7.04 µg/ml QU) as compared to wild plant and other in vitro watercress sample. Thus, in vitro culture watercress without elicitors (chitosan) and additives (casein hydrolysate, coconut water) is the richest source of glucosinolates and antioxidant in this study.
format	Thesis
qualification_name	Master of Philosophy (M.Phil.)
qualification_level	Master's degree
author	Eramartica Rubin
author_facet	Eramartica Rubin
author_sort	Eramartica Rubin
title	Determination of Gluconasturtiin and Glucotropaeolin contents and antioxidant properties of in vitro Grown Watercress (Nasturtium Officinale)
title_short	Determination of Gluconasturtiin and Glucotropaeolin contents and antioxidant properties of in vitro Grown Watercress (Nasturtium Officinale)
title_full	Determination of Gluconasturtiin and Glucotropaeolin contents and antioxidant properties of in vitro Grown Watercress (Nasturtium Officinale)
title_fullStr	Determination of Gluconasturtiin and Glucotropaeolin contents and antioxidant properties of in vitro Grown Watercress (Nasturtium Officinale)
title_full_unstemmed	Determination of Gluconasturtiin and Glucotropaeolin contents and antioxidant properties of in vitro Grown Watercress (Nasturtium Officinale)
title_sort	determination of gluconasturtiin and glucotropaeolin contents and antioxidant properties of in vitro grown watercress (nasturtium officinale)
granting_institution	Universiti Malaysia Sabah
granting_department	Faculty Of Science And Natural Resources
publishDate	2018
url	https://eprints.ums.edu.my/id/eprint/26925/1/Determination%20of%20Gluconasturtiin%20and%20Glucotropaeolin%20contents%20and%20antioxidant%20properties%20of%20in%20vitro%20%20Grown%20Watercress.pdf
_version_	1747836562204262400

Determination of Gluconasturtiin and Glucotropaeolin contents and antioxidant properties of in vitro Grown Watercress (Nasturtium Officinale)

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