Fabrication of Graphene Oxide and Graphene-Oxide/Peptide Biofilms: characterization, cell viability and differentiation potential of Wharton’s Jelly Mesenchymal stem cells

Human Wharton’s jelly mesenchymal stem cells (WJ-MSCs) treatments are being tested clinically for a range of disorders. Surface modification techniques have been instrumental in the development of biomaterials that promote cell-surface interactions. In this study, the surface of graphene oxide (GO)...

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Main Author:	Puah, Perng Yang
Format:	Thesis
Language:	English
Published:	2018
Subjects:	QC Physics
Online Access:	https://eprints.ums.edu.my/id/eprint/26953/1/Fabrication%20of%20Graphene%20Oxide%20and%20Graphene-OxidePeptide%20Biofilms%20characterization%2C%20cell%20viability%20and%20differentiation%20potential%20of%20Wharton%E2%80%99s%20Jelly%20Mesenchymal%20stem%20cells.pdf
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spelling	my-ums-ep.269532021-06-02T03:17:40Z Fabrication of Graphene Oxide and Graphene-Oxide/Peptide Biofilms: characterization, cell viability and differentiation potential of Wharton’s Jelly Mesenchymal stem cells 2018 Puah, Perng Yang QC Physics Human Wharton’s jelly mesenchymal stem cells (WJ-MSCs) treatments are being tested clinically for a range of disorders. Surface modification techniques have been instrumental in the development of biomaterials that promote cell-surface interactions. In this study, the surface of graphene oxide (GO) was modified to promote the proliferation and differentiation of Wharton’s jelly mesenchymal stem cells (WJ-MSCs). Synthesized GO was prepared through modified Hummers method, fabrication of GO film using drop-casting method and attachment of peptide to GO film through non-covalent approach, π-π and electrostatic interactions. Synthesized GO were confirmed by UV-vis, XRD and FTIR. SEM and AFM images showed that synthesized GO has curled transparent thin film with thickness of 1.10 nm. Four peptide sequences, namely Pep1 (“N”-YIGSRWYQNMIRIKVAV-“C”), Pep2 (“N”-QHREDGSYIGSRIKVAV-“C”), Pep3 (“N”-WQPPRARIYIGSRIKVAV-“C”) and Pep4 (“N”-DGEARGDSPKRSR-“C”) were designed based short peptide sequences derived from extracellular matrix (ECM) adhesion peptides. AFM results revealed the thickness of GO biofilm (0.25 mg/mL) was 82.6 nm ± 10.4 nm, corresponding to 65 – 85 layers of single layer GO. The GO biofilm (0.25 mg/mL) treated with Pep1 shows decrease in thickness as compared to non-treated GO film and the present of peptide bond in GO/Pep biofilm was confirmed by modified Lowry method. Furthermore, GO biofilms with concentration lower than 0.25 mg/mL were able to maintain the cell viability at day 5 as compared to glass coverslip. The WJ-MSCs were able to attach and growth on GO film. Increased of cell viability at day 6 was observed for all the GO/Pep biofilms as compared to GO biofilm. GO and GO/Pep biofilm allowed WJ-MSCs attachment, proliferation and increased in osteogenic differentiation capacity. Besides, the cell cultured on GO and GO/Pep biofilm able to maintain its undifferentiated stem cell characteristic. The data obtained here collectively demonstrates that the GO/Peptide biofilm assemble via non-covalent approach is a potential substrate for the adhesion, proliferation and enhance osteogenic differentiation of human WJ-MSCs. In conclusion, GO/Pep biofilms can be utilized for designing and manipulating biomaterials for stem cell, biological and tissue engineering applications. 2018 Thesis https://eprints.ums.edu.my/id/eprint/26953/ https://eprints.ums.edu.my/id/eprint/26953/1/Fabrication%20of%20Graphene%20Oxide%20and%20Graphene-OxidePeptide%20Biofilms%20characterization%2C%20cell%20viability%20and%20differentiation%20potential%20of%20Wharton%E2%80%99s%20Jelly%20Mesenchymal%20stem%20cells.pdf text en validuser mphil masters Universiti Malaysia Sabah Faculty Of Science And Natural Resources
institution	Universiti Malaysia Sabah
collection	UMS Institutional Repository
language	English
topic	QC Physics
spellingShingle	QC Physics Puah, Perng Yang Fabrication of Graphene Oxide and Graphene-Oxide/Peptide Biofilms: characterization, cell viability and differentiation potential of Wharton’s Jelly Mesenchymal stem cells
description	Human Wharton’s jelly mesenchymal stem cells (WJ-MSCs) treatments are being tested clinically for a range of disorders. Surface modification techniques have been instrumental in the development of biomaterials that promote cell-surface interactions. In this study, the surface of graphene oxide (GO) was modified to promote the proliferation and differentiation of Wharton’s jelly mesenchymal stem cells (WJ-MSCs). Synthesized GO was prepared through modified Hummers method, fabrication of GO film using drop-casting method and attachment of peptide to GO film through non-covalent approach, π-π and electrostatic interactions. Synthesized GO were confirmed by UV-vis, XRD and FTIR. SEM and AFM images showed that synthesized GO has curled transparent thin film with thickness of 1.10 nm. Four peptide sequences, namely Pep1 (“N”-YIGSRWYQNMIRIKVAV-“C”), Pep2 (“N”-QHREDGSYIGSRIKVAV-“C”), Pep3 (“N”-WQPPRARIYIGSRIKVAV-“C”) and Pep4 (“N”-DGEARGDSPKRSR-“C”) were designed based short peptide sequences derived from extracellular matrix (ECM) adhesion peptides. AFM results revealed the thickness of GO biofilm (0.25 mg/mL) was 82.6 nm ± 10.4 nm, corresponding to 65 – 85 layers of single layer GO. The GO biofilm (0.25 mg/mL) treated with Pep1 shows decrease in thickness as compared to non-treated GO film and the present of peptide bond in GO/Pep biofilm was confirmed by modified Lowry method. Furthermore, GO biofilms with concentration lower than 0.25 mg/mL were able to maintain the cell viability at day 5 as compared to glass coverslip. The WJ-MSCs were able to attach and growth on GO film. Increased of cell viability at day 6 was observed for all the GO/Pep biofilms as compared to GO biofilm. GO and GO/Pep biofilm allowed WJ-MSCs attachment, proliferation and increased in osteogenic differentiation capacity. Besides, the cell cultured on GO and GO/Pep biofilm able to maintain its undifferentiated stem cell characteristic. The data obtained here collectively demonstrates that the GO/Peptide biofilm assemble via non-covalent approach is a potential substrate for the adhesion, proliferation and enhance osteogenic differentiation of human WJ-MSCs. In conclusion, GO/Pep biofilms can be utilized for designing and manipulating biomaterials for stem cell, biological and tissue engineering applications.
format	Thesis
qualification_name	Master of Philosophy (M.Phil.)
qualification_level	Master's degree
author	Puah, Perng Yang
author_facet	Puah, Perng Yang
author_sort	Puah, Perng Yang
title	Fabrication of Graphene Oxide and Graphene-Oxide/Peptide Biofilms: characterization, cell viability and differentiation potential of Wharton’s Jelly Mesenchymal stem cells
title_short	Fabrication of Graphene Oxide and Graphene-Oxide/Peptide Biofilms: characterization, cell viability and differentiation potential of Wharton’s Jelly Mesenchymal stem cells
title_full	Fabrication of Graphene Oxide and Graphene-Oxide/Peptide Biofilms: characterization, cell viability and differentiation potential of Wharton’s Jelly Mesenchymal stem cells
title_fullStr	Fabrication of Graphene Oxide and Graphene-Oxide/Peptide Biofilms: characterization, cell viability and differentiation potential of Wharton’s Jelly Mesenchymal stem cells
title_full_unstemmed	Fabrication of Graphene Oxide and Graphene-Oxide/Peptide Biofilms: characterization, cell viability and differentiation potential of Wharton’s Jelly Mesenchymal stem cells
title_sort	fabrication of graphene oxide and graphene-oxide/peptide biofilms: characterization, cell viability and differentiation potential of wharton’s jelly mesenchymal stem cells
granting_institution	Universiti Malaysia Sabah
granting_department	Faculty Of Science And Natural Resources
publishDate	2018
url	https://eprints.ums.edu.my/id/eprint/26953/1/Fabrication%20of%20Graphene%20Oxide%20and%20Graphene-OxidePeptide%20Biofilms%20characterization%2C%20cell%20viability%20and%20differentiation%20potential%20of%20Wharton%E2%80%99s%20Jelly%20Mesenchymal%20stem%20cells.pdf
_version_	1747836562955042816

Fabrication of Graphene Oxide and Graphene-Oxide/Peptide Biofilms: characterization, cell viability and differentiation potential of Wharton’s Jelly Mesenchymal stem cells

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