Study of gut microbiome and metabolomics profiling in colorectal cancer patients

The human gastrointestinal tract harbors millions of gut microbiota. The gut microbiota has been linked to various cancers, including gastric, liver and colorectal cancers (CRC). Despite the profound knowledge and understanding of CRC development, CRC incidence rate is increasing every year and the...

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Main Author:	Lucky Goh Poh Wah
Format:	Thesis
Language:	English English
Published:	2019
Subjects:	RC254-282 Neoplasms Tumors Oncology Including cancer and carcinogens
Online Access:	https://eprints.ums.edu.my/id/eprint/32883/1/Study%20of%20gut%20microbiome%20and%20metabolomics%20profiling%20in%20colorectal%20cancer%20patients.24pages.pdf https://eprints.ums.edu.my/id/eprint/32883/2/Study%20of%20gut%20microbiome%20and%20metabolomics%20profiling%20in%20colorectal%20cancer%20patients.fulltext.pdf
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id	my-ums-ep.32883
record_format	uketd_dc
spelling	my-ums-ep.328832022-06-07T08:41:17Z Study of gut microbiome and metabolomics profiling in colorectal cancer patients 2019 Lucky Goh Poh Wah RC254-282 Neoplasms. Tumors. Oncology Including cancer and carcinogens The human gastrointestinal tract harbors millions of gut microbiota. The gut microbiota has been linked to various cancers, including gastric, liver and colorectal cancers (CRC). Despite the profound knowledge and understanding of CRC development, CRC incidence rate is increasing every year and the trend is shifting to younger age every year. This signals the need to understand the role of gut microbiota and host interactions in CRC. Therefore, this study is aimed to investigate the gut microbiota and its association to annotated host metabolites that play a potential role in the CRC with the support from gene expression analysis. A total of 35 paired case-control intestinal mucosal tissue sample were collected from CRC patients and subjected to multiple analyses. Thirty case-control tissues were subjected to gut microbiota analysis using 16S rRNA next generation sequencing. Twenty-three case-control tissues were used in metabolomics analysis using mass spectrometry. Twenty-eight case-control tissues were subjected to gene expression analysis using real-time PCR. The composition of gut microbiota, alpha diversity metrics (chaol, goods coverage, observed OTUs, Shannon index, Simpson's index, and phylogenetic distance) and beta diversity metrics (ANOSIM, PERMANOVA, PERMDISP and MRPP) shows that the gut microbiota were stable in CRC patients despite the difference in tissue pathology (cancer vs normal). Genus level analysis identified Campy/obacter from the Proteobacteria phylum was significantly (p-value < 0.05) more abundant in cases. Functional content profiling of the gut microbiota shows that glycosyltransferases, lipopolysaccharide biosynthesis proteins and its contents were also significantly (p-value < 0.05) higher in cases. Moreover, primary bile acid biosynthesis was most significantly lower (p-value < 0.05) in cases followed by bacterial toxins as well as pentose and glucuronate interconversions. 2019 Thesis https://eprints.ums.edu.my/id/eprint/32883/ https://eprints.ums.edu.my/id/eprint/32883/1/Study%20of%20gut%20microbiome%20and%20metabolomics%20profiling%20in%20colorectal%20cancer%20patients.24pages.pdf text en public https://eprints.ums.edu.my/id/eprint/32883/2/Study%20of%20gut%20microbiome%20and%20metabolomics%20profiling%20in%20colorectal%20cancer%20patients.fulltext.pdf text en validuser dphil doctoral Universiti Malaysia Sabah Faculty Of Science And Natural Resources
institution	Universiti Malaysia Sabah
collection	UMS Institutional Repository
language	English English
topic	RC254-282 Neoplasms Tumors Oncology Including cancer and carcinogens
spellingShingle	RC254-282 Neoplasms Tumors Oncology Including cancer and carcinogens Lucky Goh Poh Wah Study of gut microbiome and metabolomics profiling in colorectal cancer patients
description	The human gastrointestinal tract harbors millions of gut microbiota. The gut microbiota has been linked to various cancers, including gastric, liver and colorectal cancers (CRC). Despite the profound knowledge and understanding of CRC development, CRC incidence rate is increasing every year and the trend is shifting to younger age every year. This signals the need to understand the role of gut microbiota and host interactions in CRC. Therefore, this study is aimed to investigate the gut microbiota and its association to annotated host metabolites that play a potential role in the CRC with the support from gene expression analysis. A total of 35 paired case-control intestinal mucosal tissue sample were collected from CRC patients and subjected to multiple analyses. Thirty case-control tissues were subjected to gut microbiota analysis using 16S rRNA next generation sequencing. Twenty-three case-control tissues were used in metabolomics analysis using mass spectrometry. Twenty-eight case-control tissues were subjected to gene expression analysis using real-time PCR. The composition of gut microbiota, alpha diversity metrics (chaol, goods coverage, observed OTUs, Shannon index, Simpson's index, and phylogenetic distance) and beta diversity metrics (ANOSIM, PERMANOVA, PERMDISP and MRPP) shows that the gut microbiota were stable in CRC patients despite the difference in tissue pathology (cancer vs normal). Genus level analysis identified Campy/obacter from the Proteobacteria phylum was significantly (p-value < 0.05) more abundant in cases. Functional content profiling of the gut microbiota shows that glycosyltransferases, lipopolysaccharide biosynthesis proteins and its contents were also significantly (p-value < 0.05) higher in cases. Moreover, primary bile acid biosynthesis was most significantly lower (p-value < 0.05) in cases followed by bacterial toxins as well as pentose and glucuronate interconversions.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Lucky Goh Poh Wah
author_facet	Lucky Goh Poh Wah
author_sort	Lucky Goh Poh Wah
title	Study of gut microbiome and metabolomics profiling in colorectal cancer patients
title_short	Study of gut microbiome and metabolomics profiling in colorectal cancer patients
title_full	Study of gut microbiome and metabolomics profiling in colorectal cancer patients
title_fullStr	Study of gut microbiome and metabolomics profiling in colorectal cancer patients
title_full_unstemmed	Study of gut microbiome and metabolomics profiling in colorectal cancer patients
title_sort	study of gut microbiome and metabolomics profiling in colorectal cancer patients
granting_institution	Universiti Malaysia Sabah
granting_department	Faculty Of Science And Natural Resources
publishDate	2019
url	https://eprints.ums.edu.my/id/eprint/32883/1/Study%20of%20gut%20microbiome%20and%20metabolomics%20profiling%20in%20colorectal%20cancer%20patients.24pages.pdf https://eprints.ums.edu.my/id/eprint/32883/2/Study%20of%20gut%20microbiome%20and%20metabolomics%20profiling%20in%20colorectal%20cancer%20patients.fulltext.pdf
_version_	1747836569436291072

Study of gut microbiome and metabolomics profiling in colorectal cancer patients

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