Mass spectrometry-based metabolite profiling of wild and in vitro propagated Sabah jewel orchid Macodes limii j.j. wood & a.l. lamb

Jewel orchids are commonly known for their beautiful foliage venations and some of them are appreciated for their remarkable medicinal properties. Macodes limii is a terrestrial jewel orchid endemic to Sabah, which can only be found in specific areas with ultramafic soil. The plant is vulnerable to...

Full description

Saved in:

Bibliographic Details
Main Author:	Devina David
Format:	Thesis
Language:	English English
Published:	2023
Subjects:	QK495 Angiosperms
Online Access:	https://eprints.ums.edu.my/id/eprint/35826/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/35826/2/FULLTEXT.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-ums-ep.35826
record_format	uketd_dc
spelling	my-ums-ep.358262023-07-12T08:13:08Z Mass spectrometry-based metabolite profiling of wild and in vitro propagated Sabah jewel orchid Macodes limii j.j. wood & a.l. lamb 2023 Devina David QK495 Angiosperms Jewel orchids are commonly known for their beautiful foliage venations and some of them are appreciated for their remarkable medicinal properties. Macodes limii is a terrestrial jewel orchid endemic to Sabah, which can only be found in specific areas with ultramafic soil. The plant is vulnerable to extinction due to its specific growth requirements, besides unsustainable collection by orchid collectors for trading purposes. Despite its high ornamental value, less effort was given to protect this native species as well as to explore its chemical composition. Therefore, the current study was conducted to establish an in vitro regeneration protocol for M. limii, as well as to decipher the chemical composition of this endemic plant. The results revealed that M. limii can be regenerated via shoot tip and nodal cultures. However, to promote shoot multiplication, nodal explant was superior to shoot tip when cultured on half-MS basal media containing 0.5 mg/L of kinetin or 1.0 mg/L TDZ, which has promoted up to three shoots after 90 days of culture. Meanwhile, the total phenolic and flavonoid contents in the wild and in vitro cultivated M. limii plants revealed a strong positive correlation to the antioxidant potential by DDPH and FRAP assays. The integration of gas chromatography (GC) and liquid chromatography (LC) coupled with mass spectrometry (MS) has profiled the metabolites in the wild and in vitro cultivated M. limii plants that mostly consist of carbohydrates and their derivatives, organic acids, amino acids, fatty acids as well as flavonoids and phenolics compounds. Interestingly, this approach also has detected one potential compound, putatively annotated as 3-hydroxy-4-butanolide, a synonym to kinsenoside, which was previously reported to be the main bioactive compound in other medicinal jewel orchids. In combination with the multivariate statistical analysis, it was revealed that growing environments (wild and in vitro cultivation), as well as the age of plants (9, 18, and 27 months of culture), influenced the chemical composition of M. limii. The obtained results were the first ever reported on the micropropagation protocol as well as the metabolite profiling in M. limii and for the Macodes genus. The establishment of this in vitro propagation protocol is significant in reducing the risk of exploitation of this plant from the wild, and information on the plant metabolite profiles might serve as fundamental in understanding the metabolite variations in jewel orchids. 2023 Thesis https://eprints.ums.edu.my/id/eprint/35826/ https://eprints.ums.edu.my/id/eprint/35826/1/24%20PAGES.pdf text en public https://eprints.ums.edu.my/id/eprint/35826/2/FULLTEXT.pdf text en validuser dphil doctoral Universiti Malaysia Sabah Biotechnology Research Institute
institution	Universiti Malaysia Sabah
collection	UMS Institutional Repository
language	English English
topic	QK495 Angiosperms
spellingShingle	QK495 Angiosperms Devina David Mass spectrometry-based metabolite profiling of wild and in vitro propagated Sabah jewel orchid Macodes limii j.j. wood & a.l. lamb
description	Jewel orchids are commonly known for their beautiful foliage venations and some of them are appreciated for their remarkable medicinal properties. Macodes limii is a terrestrial jewel orchid endemic to Sabah, which can only be found in specific areas with ultramafic soil. The plant is vulnerable to extinction due to its specific growth requirements, besides unsustainable collection by orchid collectors for trading purposes. Despite its high ornamental value, less effort was given to protect this native species as well as to explore its chemical composition. Therefore, the current study was conducted to establish an in vitro regeneration protocol for M. limii, as well as to decipher the chemical composition of this endemic plant. The results revealed that M. limii can be regenerated via shoot tip and nodal cultures. However, to promote shoot multiplication, nodal explant was superior to shoot tip when cultured on half-MS basal media containing 0.5 mg/L of kinetin or 1.0 mg/L TDZ, which has promoted up to three shoots after 90 days of culture. Meanwhile, the total phenolic and flavonoid contents in the wild and in vitro cultivated M. limii plants revealed a strong positive correlation to the antioxidant potential by DDPH and FRAP assays. The integration of gas chromatography (GC) and liquid chromatography (LC) coupled with mass spectrometry (MS) has profiled the metabolites in the wild and in vitro cultivated M. limii plants that mostly consist of carbohydrates and their derivatives, organic acids, amino acids, fatty acids as well as flavonoids and phenolics compounds. Interestingly, this approach also has detected one potential compound, putatively annotated as 3-hydroxy-4-butanolide, a synonym to kinsenoside, which was previously reported to be the main bioactive compound in other medicinal jewel orchids. In combination with the multivariate statistical analysis, it was revealed that growing environments (wild and in vitro cultivation), as well as the age of plants (9, 18, and 27 months of culture), influenced the chemical composition of M. limii. The obtained results were the first ever reported on the micropropagation protocol as well as the metabolite profiling in M. limii and for the Macodes genus. The establishment of this in vitro propagation protocol is significant in reducing the risk of exploitation of this plant from the wild, and information on the plant metabolite profiles might serve as fundamental in understanding the metabolite variations in jewel orchids.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Devina David
author_facet	Devina David
author_sort	Devina David
title	Mass spectrometry-based metabolite profiling of wild and in vitro propagated Sabah jewel orchid Macodes limii j.j. wood & a.l. lamb
title_short	Mass spectrometry-based metabolite profiling of wild and in vitro propagated Sabah jewel orchid Macodes limii j.j. wood & a.l. lamb
title_full	Mass spectrometry-based metabolite profiling of wild and in vitro propagated Sabah jewel orchid Macodes limii j.j. wood & a.l. lamb
title_fullStr	Mass spectrometry-based metabolite profiling of wild and in vitro propagated Sabah jewel orchid Macodes limii j.j. wood & a.l. lamb
title_full_unstemmed	Mass spectrometry-based metabolite profiling of wild and in vitro propagated Sabah jewel orchid Macodes limii j.j. wood & a.l. lamb
title_sort	mass spectrometry-based metabolite profiling of wild and in vitro propagated sabah jewel orchid macodes limii j.j. wood & a.l. lamb
granting_institution	Universiti Malaysia Sabah
granting_department	Biotechnology Research Institute
publishDate	2023
url	https://eprints.ums.edu.my/id/eprint/35826/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/35826/2/FULLTEXT.pdf
_version_	1776102895464218624

Mass spectrometry-based metabolite profiling of wild and in vitro propagated Sabah jewel orchid Macodes limii j.j. wood & a.l. lamb

Similar Items