A comparative study on the effectiveness of adjunct online learning tool between conventional and gamified e-learning in enhancing electrocardiogram competency among medical students
Homeotic genes are known to play important roles in the development of fruits and flowers. Most of these genes belong to a large family of regulatory genes that have a characteristic DNA binding domain known as the MADS-box. As a pioneer effort in the study of homeotic genes from banana, a full leng...
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my-ums-ep.381532024-02-08T07:48:55Z A comparative study on the effectiveness of adjunct online learning tool between conventional and gamified e-learning in enhancing electrocardiogram competency among medical students 2019 May Honey Ohn R735-854 Medical education. Medical schools. Research Homeotic genes are known to play important roles in the development of fruits and flowers. Most of these genes belong to a large family of regulatory genes that have a characteristic DNA binding domain known as the MADS-box. As a pioneer effort in the study of homeotic genes from banana, a full length MADS-box cDNA from an inflorescence of Musa acuminata var. Berangan designated as MADSJ has been successfully isolated and characterized. The approach of this research was by isolating Poly A+ mRNA from the inflorescence of Pisang Berangan using oligo-dT magnetic beads. First strand cDNA was synthesized using a dT18 anchored primer directly onto those beads. A degenerate primer designed by aligning the sequences of the 180bp MADS domain from known MADS-box genes was used as the forward primer to perform a 3' RACE. This generated a complete coding sequence. To obtain the 5' untranslated region on the N terminal side of the MADS-box domain a 5' RACE was carried out. NCBI BLAST analyses were done on these sequences to confirm authenticity to known MADS-box genes. The PHYLIP package was used to further analyze these sequences. The putative 244 amino acid sequence deduced from this research supporting the MADS-box region and the K domain of the corresponding gene suggests that it is a member of the Type II family. MADSJ belongs to the AGL clade and is a member of the SEPALLA TA subfamily of MADS-box transcription factors. 2019 Thesis https://eprints.ums.edu.my/id/eprint/38153/ https://eprints.ums.edu.my/id/eprint/38153/1/24%20PAGES.pdf text en public https://eprints.ums.edu.my/id/eprint/38153/2/FULLTEXT.pdf text en validuser dphil doctoral Universiti Malaysia Sabah Fakulti Perubatan Sains dan Kesihatan |
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R735-854 Medical education Medical schools Research May Honey Ohn A comparative study on the effectiveness of adjunct online learning tool between conventional and gamified e-learning in enhancing electrocardiogram competency among medical students |
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Homeotic genes are known to play important roles in the development of fruits and flowers. Most of these genes belong to a large family of regulatory genes that have a characteristic DNA binding domain known as the MADS-box. As a pioneer effort in the study of homeotic genes from banana, a full length MADS-box cDNA from an inflorescence of Musa acuminata var. Berangan designated as MADSJ has been successfully isolated and characterized. The approach of this research was by isolating Poly A+ mRNA from the inflorescence of Pisang Berangan using oligo-dT magnetic beads. First strand cDNA was synthesized using a dT18 anchored primer directly onto those beads. A degenerate primer designed by aligning the sequences of the 180bp MADS domain from known MADS-box genes was used as the forward primer to perform a 3' RACE. This generated a complete coding sequence. To obtain the 5' untranslated region on the N terminal side of the MADS-box domain a 5' RACE was carried out. NCBI BLAST analyses were done on these sequences to confirm authenticity to known MADS-box genes. The PHYLIP package was used to further analyze these sequences. The putative 244 amino acid sequence deduced from this research supporting the MADS-box region and the K domain of the corresponding gene suggests that it is a member of the Type II family. MADSJ belongs to the AGL clade and is a member of the SEPALLA TA subfamily of MADS-box transcription factors. |
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Thesis |
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Doctor of Philosophy (PhD.) |
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Doctorate |
author |
May Honey Ohn |
author_facet |
May Honey Ohn |
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May Honey Ohn |
title |
A comparative study on the effectiveness of adjunct online learning tool between conventional and gamified e-learning in enhancing electrocardiogram competency among medical students |
title_short |
A comparative study on the effectiveness of adjunct online learning tool between conventional and gamified e-learning in enhancing electrocardiogram competency among medical students |
title_full |
A comparative study on the effectiveness of adjunct online learning tool between conventional and gamified e-learning in enhancing electrocardiogram competency among medical students |
title_fullStr |
A comparative study on the effectiveness of adjunct online learning tool between conventional and gamified e-learning in enhancing electrocardiogram competency among medical students |
title_full_unstemmed |
A comparative study on the effectiveness of adjunct online learning tool between conventional and gamified e-learning in enhancing electrocardiogram competency among medical students |
title_sort |
comparative study on the effectiveness of adjunct online learning tool between conventional and gamified e-learning in enhancing electrocardiogram competency among medical students |
granting_institution |
Universiti Malaysia Sabah |
granting_department |
Fakulti Perubatan Sains dan Kesihatan |
publishDate |
2019 |
url |
https://eprints.ums.edu.my/id/eprint/38153/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/38153/2/FULLTEXT.pdf |
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