Phylogenetic relationships of chingia, sphaerostephanos and christella groups (Thelypteridaceae) from Sabah using rbcL gene sequences

This study determined the phylogenetic relationship of Chingia, Sphaerostephanos and Christella groups and examined the grouping of the genera based on the resultant rbcl analyses in comparison to the morphological analyses, which were based on morphological descriptions provided by Holttum. The stu...

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Main Author:	Jacqueline Joseph
Format:	Thesis
Language:	English English
Published:	2010
Subjects:	QK504-(638) Cryptogams
Online Access:	https://eprints.ums.edu.my/id/eprint/38496/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/38496/2/FULLTEXT.pdf
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spelling	my-ums-ep.384962024-04-15T07:25:57Z Phylogenetic relationships of chingia, sphaerostephanos and christella groups (Thelypteridaceae) from Sabah using rbcL gene sequences 2010 Jacqueline Joseph QK504-(638) Cryptogams This study determined the phylogenetic relationship of Chingia, Sphaerostephanos and Christella groups and examined the grouping of the genera based on the resultant rbcl analyses in comparison to the morphological analyses, which were based on morphological descriptions provided by Holttum. The study sites consisted of Crocker Range Park and few of the West Coast Sabah area. In this phylogenetic study, rbcl gene marker was used to infer phylogenetic relationships of the study groups whereas morphological analyses was used to check the relationship based on the morphological characters gathered from Holttum. In the phylogenetic study of Chingia, Sphaerostephanos and Christella groups, 977 base pairs of DNA sequences were obtained from 32 samples representing the 3 groups of genera. The phylogenetic relationships and morphological analyses revealed that Sphaerostephanos is a taxonomically-problematic group especially for Pronephrium and Sphaerostephanos. The phylogenetic analyses found that there were two clades derived from the primitive Sphaerostephanos group, consisted of a monophyly advanced Sphaerostephanos clade and a monophyly clade of Chingia group and Christella group. Morphological analyses showed that Amphineuron and Christel/a were well-clustered into the Christella group. However, it was found that some of the Christel/a spp. ( C. subpubescens and C. papi/io) were established a lineage to Chingia group in the rbcl analyses. The grouping of Chingia and P/esioneuron into the Chingia group gained support from the morphological analyses but not completely supported by the rbd.. analyses since Chingia spp. ( C. c/avipi/osa and C. perrigida) were clustered into Christella group. Chingia and Christel/a were found to form a monophyletic clade together in both analyses. However, rbcl analyses does not support this grouping very well with <70% of bootstrap probability, and showed that Chingia group and Christella group were almost separated into two independent monophyly clade. The separation was in agreement with the morphological analyses that Christel/a clade and Chingia clade were split from each other to form their own monophyletic clade. It can be seen that Chingia group and Christella group were derived from a monophyletic group together and gradually evolved to form two independent groups. Pneumatopteris was verified being the genera under the Sphaerostephanos group since Pneumatopteriswas found appear in the monophyletic advanced Sphaerostephanos clade in the rbd.. analyses. Despite being that, the grouping of genera within Sphaerostephanos group cannot be displayed clearly. This could be due to the insufficiency of morphological characters used in morphological analyses were not strong enough to separate or distinguish certain genera into the Sphaerostephanos group. Genetic factors were also believed to influence the outcomes of rbcl analyses on the Sphaerostephanos group. Undoubtedly, more details studies should be carried out to support the proposed conclusions since this is the first time phylogenetic study conducted on Chingia, Sphaerostephanos and Christella groups of genera. 2010 Thesis https://eprints.ums.edu.my/id/eprint/38496/ https://eprints.ums.edu.my/id/eprint/38496/1/24%20PAGES.pdf text en public https://eprints.ums.edu.my/id/eprint/38496/2/FULLTEXT.pdf text en validuser masters Universiti Malaysia Sabah Institut Biologi Tropika dan Pemuliharaan
institution	Universiti Malaysia Sabah
collection	UMS Institutional Repository
language	English English
topic	QK504-(638) Cryptogams
spellingShingle	QK504-(638) Cryptogams Jacqueline Joseph Phylogenetic relationships of chingia, sphaerostephanos and christella groups (Thelypteridaceae) from Sabah using rbcL gene sequences
description	This study determined the phylogenetic relationship of Chingia, Sphaerostephanos and Christella groups and examined the grouping of the genera based on the resultant rbcl analyses in comparison to the morphological analyses, which were based on morphological descriptions provided by Holttum. The study sites consisted of Crocker Range Park and few of the West Coast Sabah area. In this phylogenetic study, rbcl gene marker was used to infer phylogenetic relationships of the study groups whereas morphological analyses was used to check the relationship based on the morphological characters gathered from Holttum. In the phylogenetic study of Chingia, Sphaerostephanos and Christella groups, 977 base pairs of DNA sequences were obtained from 32 samples representing the 3 groups of genera. The phylogenetic relationships and morphological analyses revealed that Sphaerostephanos is a taxonomically-problematic group especially for Pronephrium and Sphaerostephanos. The phylogenetic analyses found that there were two clades derived from the primitive Sphaerostephanos group, consisted of a monophyly advanced Sphaerostephanos clade and a monophyly clade of Chingia group and Christella group. Morphological analyses showed that Amphineuron and Christel/a were well-clustered into the Christella group. However, it was found that some of the Christel/a spp. ( C. subpubescens and C. papi/io) were established a lineage to Chingia group in the rbcl analyses. The grouping of Chingia and P/esioneuron into the Chingia group gained support from the morphological analyses but not completely supported by the rbd.. analyses since Chingia spp. ( C. c/avipi/osa and C. perrigida) were clustered into Christella group. Chingia and Christel/a were found to form a monophyletic clade together in both analyses. However, rbcl analyses does not support this grouping very well with <70% of bootstrap probability, and showed that Chingia group and Christella group were almost separated into two independent monophyly clade. The separation was in agreement with the morphological analyses that Christel/a clade and Chingia clade were split from each other to form their own monophyletic clade. It can be seen that Chingia group and Christella group were derived from a monophyletic group together and gradually evolved to form two independent groups. Pneumatopteris was verified being the genera under the Sphaerostephanos group since Pneumatopteriswas found appear in the monophyletic advanced Sphaerostephanos clade in the rbd.. analyses. Despite being that, the grouping of genera within Sphaerostephanos group cannot be displayed clearly. This could be due to the insufficiency of morphological characters used in morphological analyses were not strong enough to separate or distinguish certain genera into the Sphaerostephanos group. Genetic factors were also believed to influence the outcomes of rbcl analyses on the Sphaerostephanos group. Undoubtedly, more details studies should be carried out to support the proposed conclusions since this is the first time phylogenetic study conducted on Chingia, Sphaerostephanos and Christella groups of genera.
format	Thesis
qualification_level	Master's degree
author	Jacqueline Joseph
author_facet	Jacqueline Joseph
author_sort	Jacqueline Joseph
title	Phylogenetic relationships of chingia, sphaerostephanos and christella groups (Thelypteridaceae) from Sabah using rbcL gene sequences
title_short	Phylogenetic relationships of chingia, sphaerostephanos and christella groups (Thelypteridaceae) from Sabah using rbcL gene sequences
title_full	Phylogenetic relationships of chingia, sphaerostephanos and christella groups (Thelypteridaceae) from Sabah using rbcL gene sequences
title_fullStr	Phylogenetic relationships of chingia, sphaerostephanos and christella groups (Thelypteridaceae) from Sabah using rbcL gene sequences
title_full_unstemmed	Phylogenetic relationships of chingia, sphaerostephanos and christella groups (Thelypteridaceae) from Sabah using rbcL gene sequences
title_sort	phylogenetic relationships of chingia, sphaerostephanos and christella groups (thelypteridaceae) from sabah using rbcl gene sequences
granting_institution	Universiti Malaysia Sabah
granting_department	Institut Biologi Tropika dan Pemuliharaan
publishDate	2010
url	https://eprints.ums.edu.my/id/eprint/38496/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/38496/2/FULLTEXT.pdf
_version_	1804890316574031872

Phylogenetic relationships of chingia, sphaerostephanos and christella groups (Thelypteridaceae) from Sabah using rbcL gene sequences

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