Detection of community-acquired methicillin-resistant staphylococcus aureus (CA-MRSA) transmission in the local community using molecular typing techniques

Since the 1990s, Community-acquired Methicillin-Resistant Staphylococcus aureus (CA-MRSA) has caused infections in population without healthcare-associated risk factors. Unlike nosocomial MRSA, CA-MRSA harbors pv/gene and SCCmedV subtype, V, or untypable SCCmec. CA-MRSA is usually associated with su...

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Main Author:	Lia Natasha Amit
Format:	Thesis
Language:	English English
Published:	2018
Subjects:	QR1-502 Microbiology
Online Access:	https://eprints.ums.edu.my/id/eprint/38510/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/38510/2/FULLTEXT.pdf
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spelling	my-ums-ep.385102024-04-23T06:50:50Z Detection of community-acquired methicillin-resistant staphylococcus aureus (CA-MRSA) transmission in the local community using molecular typing techniques 2018 Lia Natasha Amit QR1-502 Microbiology Since the 1990s, Community-acquired Methicillin-Resistant Staphylococcus aureus (CA-MRSA) has caused infections in population without healthcare-associated risk factors. Unlike nosocomial MRSA, CA-MRSA harbors pv/gene and SCCmedV subtype, V, or untypable SCCmec. CA-MRSA is usually associated with suppurative infections among children. Children are more predisposed to be CA-MRSA carriers and transmit CA-MRSA to family members. This study aimed to elucidate CA-MRSA transmission in Sabah local community by molecular characterization of MRSA abscess isolates from pediatric patients in Hospital Likas and MRSA nasal isolates from participating family members within a one-year period. Thirty-seven MRSA abscess isolates of index patients which were identified from the cefoxitin antibiotic susceptibility tests were collected from Pathology Laboratory. Nineteen nasal swab samples were collected from family members and processed by the culture on blood agar, Gram staining, catalase test, coagulase test, and DNAse test for 5. aureus identification. PCR of mecA gene were conducted on 37 MRSA abscess isolates and 14 S. aureus nasal isolates. PCR of pv/gene and PCR for SCCmectyping were conducted on MRSA isolates. PVL positive CA-MRSA isolates of index patients and family members which did not harbor similar SCCmec elements were tested for spa typing. Patients' age, ethnicity, and site of infection were recorded into patient information sheets. Research survey were given to participating family members. This study validated the methicillin resistance status of 37 MRSA abscess isolates and 14 nasal 5. aureus based on the presence of mecA gene. This study supported previous studies where CA-MRSA predominated in abscess infections of pediatric patients. From 37 MRSA abscess isolates, 33 isolates were confirmed as CA-MRSA based on the presence of pv/gene and SCCmedVa (n=12), IVc (n=l), V (n=l), or untypable SCCmec(n=19). From 37 index cases, only CA-MRSA isolates from 2 cases showed similarities of spa genes. CA-MRSA abscess isolates, Al 167 (S2) and AS413 (S7), shared similar spa types with CA-MRSA isolates from family members, F1167 (S2.1) and F5413 (S7.1), respectively. From 37 CA-MRSA index cases, 18 patients had abscesses on scalp suggesting that infant locomotion development, skin-to-skin contact, and contaminated household surfaces leads to CA-MRSA transmission. This study has extended the understanding of the CA-MRSA transmission. The data herein could be important in developing antidotes for CA-MRSA infections in Malaysia. 2018 Thesis https://eprints.ums.edu.my/id/eprint/38510/ https://eprints.ums.edu.my/id/eprint/38510/1/24%20PAGES.pdf text en public https://eprints.ums.edu.my/id/eprint/38510/2/FULLTEXT.pdf text en validuser masters Universiti Malaysia Sabah Institut Penyelidikan Bioteknologi
institution	Universiti Malaysia Sabah
collection	UMS Institutional Repository
language	English English
topic	QR1-502 Microbiology
spellingShingle	QR1-502 Microbiology Lia Natasha Amit Detection of community-acquired methicillin-resistant staphylococcus aureus (CA-MRSA) transmission in the local community using molecular typing techniques
description	Since the 1990s, Community-acquired Methicillin-Resistant Staphylococcus aureus (CA-MRSA) has caused infections in population without healthcare-associated risk factors. Unlike nosocomial MRSA, CA-MRSA harbors pv/gene and SCCmedV subtype, V, or untypable SCCmec. CA-MRSA is usually associated with suppurative infections among children. Children are more predisposed to be CA-MRSA carriers and transmit CA-MRSA to family members. This study aimed to elucidate CA-MRSA transmission in Sabah local community by molecular characterization of MRSA abscess isolates from pediatric patients in Hospital Likas and MRSA nasal isolates from participating family members within a one-year period. Thirty-seven MRSA abscess isolates of index patients which were identified from the cefoxitin antibiotic susceptibility tests were collected from Pathology Laboratory. Nineteen nasal swab samples were collected from family members and processed by the culture on blood agar, Gram staining, catalase test, coagulase test, and DNAse test for 5. aureus identification. PCR of mecA gene were conducted on 37 MRSA abscess isolates and 14 S. aureus nasal isolates. PCR of pv/gene and PCR for SCCmectyping were conducted on MRSA isolates. PVL positive CA-MRSA isolates of index patients and family members which did not harbor similar SCCmec elements were tested for spa typing. Patients' age, ethnicity, and site of infection were recorded into patient information sheets. Research survey were given to participating family members. This study validated the methicillin resistance status of 37 MRSA abscess isolates and 14 nasal 5. aureus based on the presence of mecA gene. This study supported previous studies where CA-MRSA predominated in abscess infections of pediatric patients. From 37 MRSA abscess isolates, 33 isolates were confirmed as CA-MRSA based on the presence of pv/gene and SCCmedVa (n=12), IVc (n=l), V (n=l), or untypable SCCmec(n=19). From 37 index cases, only CA-MRSA isolates from 2 cases showed similarities of spa genes. CA-MRSA abscess isolates, Al 167 (S2) and AS413 (S7), shared similar spa types with CA-MRSA isolates from family members, F1167 (S2.1) and F5413 (S7.1), respectively. From 37 CA-MRSA index cases, 18 patients had abscesses on scalp suggesting that infant locomotion development, skin-to-skin contact, and contaminated household surfaces leads to CA-MRSA transmission. This study has extended the understanding of the CA-MRSA transmission. The data herein could be important in developing antidotes for CA-MRSA infections in Malaysia.
format	Thesis
qualification_level	Master's degree
author	Lia Natasha Amit
author_facet	Lia Natasha Amit
author_sort	Lia Natasha Amit
title	Detection of community-acquired methicillin-resistant staphylococcus aureus (CA-MRSA) transmission in the local community using molecular typing techniques
title_short	Detection of community-acquired methicillin-resistant staphylococcus aureus (CA-MRSA) transmission in the local community using molecular typing techniques
title_full	Detection of community-acquired methicillin-resistant staphylococcus aureus (CA-MRSA) transmission in the local community using molecular typing techniques
title_fullStr	Detection of community-acquired methicillin-resistant staphylococcus aureus (CA-MRSA) transmission in the local community using molecular typing techniques
title_full_unstemmed	Detection of community-acquired methicillin-resistant staphylococcus aureus (CA-MRSA) transmission in the local community using molecular typing techniques
title_sort	detection of community-acquired methicillin-resistant staphylococcus aureus (ca-mrsa) transmission in the local community using molecular typing techniques
granting_institution	Universiti Malaysia Sabah
granting_department	Institut Penyelidikan Bioteknologi
publishDate	2018
url	https://eprints.ums.edu.my/id/eprint/38510/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/38510/2/FULLTEXT.pdf
_version_	1804890319590785024

Detection of community-acquired methicillin-resistant staphylococcus aureus (CA-MRSA) transmission in the local community using molecular typing techniques

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