Development of acetylcholinesterase (AChE) biosensor for determination of carbaryl pesticide in Brassica oleracea Var.capitata L. (Cabbage) and Brassica oleracea Var. italica L. (Broccoli)

Carbary! pesticide widely applied as insecticide to protect crops against pests in agriculture. Overuses of carbaryl are hazardous as it interrupts human central nervous system by causing signal transmission to be impaired. Because of the hazardous effects resulted by the prolonged exposure to the p...

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Main Author:	Samsidar Anwar
Format:	Thesis
Language:	English English
Published:	2018
Subjects:	QP501-801 Animal biochemistry
Online Access:	https://eprints.ums.edu.my/id/eprint/38540/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/38540/2/FULLTEXT.pdf
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spelling	my-ums-ep.385402024-04-29T02:34:35Z Development of acetylcholinesterase (AChE) biosensor for determination of carbaryl pesticide in Brassica oleracea Var.capitata L. (Cabbage) and Brassica oleracea Var. italica L. (Broccoli) 2018 Samsidar Anwar QP501-801 Animal biochemistry Carbary! pesticide widely applied as insecticide to protect crops against pests in agriculture. Overuses of carbaryl are hazardous as it interrupts human central nervous system by causing signal transmission to be impaired. Because of the hazardous effects resulted by the prolonged exposure to the pesticide, rapid detection of pesticide is highly concerned. Various detection techniques have been developed including chromatographic based method, capillary electrophoresis and spectroscopy. Such techniques are provided excellent sensitivity and accuracy but require expensive instrument, competent handler besides takes longer time of process. In this study, a new electrochemical method was developed based on immobilization of acetylcholinesterase (AChE) enzyme via glutaraldehyde (GA) as a cross linking agent onto glassy carbon electrode (GCE) modified by the homogenously dispersed of multi-walled carbon nanotubes (MWCNTs) and gold nanoparticles (AuNPs) in chitosan (CS) for detection of carbaryl pesticide. The combination of all these nanomaterials are provided great platform for the AChE activity due to their excellent electrochemical properties. The morphological characteristics of hybrid nanomaterials were observed by using SEM, EDX and TE M. The electrochemical behaviours showed the current signals increasing in order: bare GCE < CS/GCE < CS/MWCNTs/GCE < CS/MWCNTs/ AuNPs/GCE. The optimal conditions of the developed biosensor were found of pH 7 for Tris-HCI buffer (0.1 M), 20 s for reaction time, 0.1 V/s for scan rate and 6 min for inhibition time. Based on the inhibition of carbaryl on AChE activity, the DPV was detected in the ranges from 0.1 to 8 mg/L. Low detection limit was found of 4. 95 x 10-3 mg/L with good repeatability, reproducibility and storage stability on the carbaryl detection. The determination of carbaryl in real vegetable samples of Brassica oleracea var. L. capitata (Cabbage) and Brassica oleracea var. L. italica (Broccoli) showed good recovery rates between 73. 7 to 102.8% with relative standard deviation less than 7%. Thus, the developed AChE biosensor is showed a rapid and high sensitive tool for determination of carbaryl in vegetable samples. 2018 Thesis https://eprints.ums.edu.my/id/eprint/38540/ https://eprints.ums.edu.my/id/eprint/38540/1/24%20PAGES.pdf text en public https://eprints.ums.edu.my/id/eprint/38540/2/FULLTEXT.pdf text en validuser masters Universiti Malaysia Sabah Institut Penyelidikan Bioteknologi
institution	Universiti Malaysia Sabah
collection	UMS Institutional Repository
language	English English
topic	QP501-801 Animal biochemistry
spellingShingle	QP501-801 Animal biochemistry Samsidar Anwar Development of acetylcholinesterase (AChE) biosensor for determination of carbaryl pesticide in Brassica oleracea Var.capitata L. (Cabbage) and Brassica oleracea Var. italica L. (Broccoli)
description	Carbary! pesticide widely applied as insecticide to protect crops against pests in agriculture. Overuses of carbaryl are hazardous as it interrupts human central nervous system by causing signal transmission to be impaired. Because of the hazardous effects resulted by the prolonged exposure to the pesticide, rapid detection of pesticide is highly concerned. Various detection techniques have been developed including chromatographic based method, capillary electrophoresis and spectroscopy. Such techniques are provided excellent sensitivity and accuracy but require expensive instrument, competent handler besides takes longer time of process. In this study, a new electrochemical method was developed based on immobilization of acetylcholinesterase (AChE) enzyme via glutaraldehyde (GA) as a cross linking agent onto glassy carbon electrode (GCE) modified by the homogenously dispersed of multi-walled carbon nanotubes (MWCNTs) and gold nanoparticles (AuNPs) in chitosan (CS) for detection of carbaryl pesticide. The combination of all these nanomaterials are provided great platform for the AChE activity due to their excellent electrochemical properties. The morphological characteristics of hybrid nanomaterials were observed by using SEM, EDX and TE M. The electrochemical behaviours showed the current signals increasing in order: bare GCE < CS/GCE < CS/MWCNTs/GCE < CS/MWCNTs/ AuNPs/GCE. The optimal conditions of the developed biosensor were found of pH 7 for Tris-HCI buffer (0.1 M), 20 s for reaction time, 0.1 V/s for scan rate and 6 min for inhibition time. Based on the inhibition of carbaryl on AChE activity, the DPV was detected in the ranges from 0.1 to 8 mg/L. Low detection limit was found of 4. 95 x 10-3 mg/L with good repeatability, reproducibility and storage stability on the carbaryl detection. The determination of carbaryl in real vegetable samples of Brassica oleracea var. L. capitata (Cabbage) and Brassica oleracea var. L. italica (Broccoli) showed good recovery rates between 73. 7 to 102.8% with relative standard deviation less than 7%. Thus, the developed AChE biosensor is showed a rapid and high sensitive tool for determination of carbaryl in vegetable samples.
format	Thesis
qualification_level	Master's degree
author	Samsidar Anwar
author_facet	Samsidar Anwar
author_sort	Samsidar Anwar
title	Development of acetylcholinesterase (AChE) biosensor for determination of carbaryl pesticide in Brassica oleracea Var.capitata L. (Cabbage) and Brassica oleracea Var. italica L. (Broccoli)
title_short	Development of acetylcholinesterase (AChE) biosensor for determination of carbaryl pesticide in Brassica oleracea Var.capitata L. (Cabbage) and Brassica oleracea Var. italica L. (Broccoli)
title_full	Development of acetylcholinesterase (AChE) biosensor for determination of carbaryl pesticide in Brassica oleracea Var.capitata L. (Cabbage) and Brassica oleracea Var. italica L. (Broccoli)
title_fullStr	Development of acetylcholinesterase (AChE) biosensor for determination of carbaryl pesticide in Brassica oleracea Var.capitata L. (Cabbage) and Brassica oleracea Var. italica L. (Broccoli)
title_full_unstemmed	Development of acetylcholinesterase (AChE) biosensor for determination of carbaryl pesticide in Brassica oleracea Var.capitata L. (Cabbage) and Brassica oleracea Var. italica L. (Broccoli)
title_sort	development of acetylcholinesterase (ache) biosensor for determination of carbaryl pesticide in brassica oleracea var.capitata l. (cabbage) and brassica oleracea var. italica l. (broccoli)
granting_institution	Universiti Malaysia Sabah
granting_department	Institut Penyelidikan Bioteknologi
publishDate	2018
url	https://eprints.ums.edu.my/id/eprint/38540/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/38540/2/FULLTEXT.pdf
_version_	1804890325994438656

Development of acetylcholinesterase (AChE) biosensor for determination of carbaryl pesticide in Brassica oleracea Var.capitata L. (Cabbage) and Brassica oleracea Var. italica L. (Broccoli)

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