Characterization of Trichoderma isolated and their lignocellulolytic activities

Trichoderma is a cosmopolitan fungus that prevalent in the soil and other diverse habitats. It has gained vast economical importance's because industrial enzymes production, antifungal, antibiotics, biocontrol agents and plant growth promoter. Trichoderma species produced the lignocellulolytic...

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Main Author:	Azriah Asis
Format:	Thesis
Language:	English English
Published:	2017
Subjects:	QK504-(638) Cryptogams
Online Access:	https://eprints.ums.edu.my/id/eprint/38583/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/38583/2/FULLTEXT.pdf
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spelling	my-ums-ep.385832024-05-02T08:41:45Z Characterization of Trichoderma isolated and their lignocellulolytic activities 2017 Azriah Asis QK504-(638) Cryptogams Trichoderma is a cosmopolitan fungus that prevalent in the soil and other diverse habitats. It has gained vast economical importance's because industrial enzymes production, antifungal, antibiotics, biocontrol agents and plant growth promoter. Trichoderma species produced the lignocellulolytic enzymes activities that assisted in the degradation of woody lignocellulose materials for industrial applications. Therefore, an accurately identification of Trichoderma isolates at the species level is highly desirable. In this study, the main aim to accurately identify of Trichoderma isolates at the species level based on the morphological characteristics, lignocellulolytic enzyme activities and multilocus gene sequencing based on the Internal Transcribed Spacers 1 and 2 (ITS1 and ITS2) regions of the rDNA, a partial sequence of the Translation Elongation Factor 1-alpha ( tefl) and calmodulin (ea/). A total of 53 isolates of Trichoderma were isolated from wet paddy field in Tuaran, 5abah, Malaysia. On the combination of morphological characteristics and multilocus gene sequencing analysis were positively identified three Trichoderma species, namely T. asperellum, T. harzianum and T. reesei. The phylogenetic relationships were constructed based on the Internal Transcribed Spacers 1 and 2 (ITS1 and ITS2) regions of the rDNA gene, a partial sequence of the Translation Elongation Factor 1-alpha (tefl) and calmodulin gene (ea/) by using UPGMA method and found three sections such as T. asperellum in the "Trichoderma'' section, T. harzianum in the "Pachybasium'' section, and T. reesei in the "Long1brachiatuni' section. Mycelial growth and biomass yield among three species isolates were examined on Potato Dextrose Agar and Potato Dextrose Broth, respectively, with different temperatures; 20, 25, 30, 35 and 40 °C. T. asperellum isolates were grown very well of mycelia growth and produced the highest biomass yield at 30 °c, followed by T. harzianum and T. reesei. The lignocellulolytic activities were assayed based on their ability to develop dark brown pigments, yellow halo zone, and clear white zone on tannic acid media (TAM) for lignin, Jensen Media (JM) for cellulose and modified Melin-Nokrans media (MMNM) for starch. The diameters of halo zones were measured for the analysis of their ability in degrading lignin, cellulose, and starch. The best seven Trichoderma isolates [51(9)10-1(3), E3(6)10-1(2), W2(2)10-1(2), 53(1)10-1(1 ), N2( 4)10-2(3), N2(2)10-1(2) dan 53(6)10-1(2)] were found the potential lignocellulolytic agents based on the diameter of dark brown pigments and halo zones formed. Trichoderma species are found to synthesize polyphenol oxidase, endoglucanases, and are able to hydrolyze starch to glucose in the three different media. Thus, the potential Trichoderma isolates can be further applied as biocontrol agents in controlling disease and increasing yield of agricultural crops. 2017 Thesis https://eprints.ums.edu.my/id/eprint/38583/ https://eprints.ums.edu.my/id/eprint/38583/1/24%20PAGES.pdf text en public https://eprints.ums.edu.my/id/eprint/38583/2/FULLTEXT.pdf text en validuser masters Universiti Malaysia Sabah Institut Penyelidikan Bioteknologi
institution	Universiti Malaysia Sabah
collection	UMS Institutional Repository
language	English English
topic	QK504-(638) Cryptogams
spellingShingle	QK504-(638) Cryptogams Azriah Asis Characterization of Trichoderma isolated and their lignocellulolytic activities
description	Trichoderma is a cosmopolitan fungus that prevalent in the soil and other diverse habitats. It has gained vast economical importance's because industrial enzymes production, antifungal, antibiotics, biocontrol agents and plant growth promoter. Trichoderma species produced the lignocellulolytic enzymes activities that assisted in the degradation of woody lignocellulose materials for industrial applications. Therefore, an accurately identification of Trichoderma isolates at the species level is highly desirable. In this study, the main aim to accurately identify of Trichoderma isolates at the species level based on the morphological characteristics, lignocellulolytic enzyme activities and multilocus gene sequencing based on the Internal Transcribed Spacers 1 and 2 (ITS1 and ITS2) regions of the rDNA, a partial sequence of the Translation Elongation Factor 1-alpha ( tefl) and calmodulin (ea/). A total of 53 isolates of Trichoderma were isolated from wet paddy field in Tuaran, 5abah, Malaysia. On the combination of morphological characteristics and multilocus gene sequencing analysis were positively identified three Trichoderma species, namely T. asperellum, T. harzianum and T. reesei. The phylogenetic relationships were constructed based on the Internal Transcribed Spacers 1 and 2 (ITS1 and ITS2) regions of the rDNA gene, a partial sequence of the Translation Elongation Factor 1-alpha (tefl) and calmodulin gene (ea/) by using UPGMA method and found three sections such as T. asperellum in the "Trichoderma'' section, T. harzianum in the "Pachybasium'' section, and T. reesei in the "Long1brachiatuni' section. Mycelial growth and biomass yield among three species isolates were examined on Potato Dextrose Agar and Potato Dextrose Broth, respectively, with different temperatures; 20, 25, 30, 35 and 40 °C. T. asperellum isolates were grown very well of mycelia growth and produced the highest biomass yield at 30 °c, followed by T. harzianum and T. reesei. The lignocellulolytic activities were assayed based on their ability to develop dark brown pigments, yellow halo zone, and clear white zone on tannic acid media (TAM) for lignin, Jensen Media (JM) for cellulose and modified Melin-Nokrans media (MMNM) for starch. The diameters of halo zones were measured for the analysis of their ability in degrading lignin, cellulose, and starch. The best seven Trichoderma isolates [51(9)10-1(3), E3(6)10-1(2), W2(2)10-1(2), 53(1)10-1(1 ), N2( 4)10-2(3), N2(2)10-1(2) dan 53(6)10-1(2)] were found the potential lignocellulolytic agents based on the diameter of dark brown pigments and halo zones formed. Trichoderma species are found to synthesize polyphenol oxidase, endoglucanases, and are able to hydrolyze starch to glucose in the three different media. Thus, the potential Trichoderma isolates can be further applied as biocontrol agents in controlling disease and increasing yield of agricultural crops.
format	Thesis
qualification_level	Master's degree
author	Azriah Asis
author_facet	Azriah Asis
author_sort	Azriah Asis
title	Characterization of Trichoderma isolated and their lignocellulolytic activities
title_short	Characterization of Trichoderma isolated and their lignocellulolytic activities
title_full	Characterization of Trichoderma isolated and their lignocellulolytic activities
title_fullStr	Characterization of Trichoderma isolated and their lignocellulolytic activities
title_full_unstemmed	Characterization of Trichoderma isolated and their lignocellulolytic activities
title_sort	characterization of trichoderma isolated and their lignocellulolytic activities
granting_institution	Universiti Malaysia Sabah
granting_department	Institut Penyelidikan Bioteknologi
publishDate	2017
url	https://eprints.ums.edu.my/id/eprint/38583/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/38583/2/FULLTEXT.pdf
_version_	1804890329802866688

Characterization of Trichoderma isolated and their lignocellulolytic activities

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