Isolation and characterisation of fibrolytic and lipolytic enzyme-producing bacteria from oil palm empty fruit bunch compost

Palm kernel pulp (PKC) is one of the important waste products of palm oil. PKC has been used as feed for ruminant animals and poultry and ducks. The use of PKC in general poultry and duck feed is limited due to the anti-nutritional properties such as galactomannan and xylan found in PKC. These anti-...

Full description

Saved in:

Bibliographic Details
Main Author:	Elaine Remi Anak Douglas Telajan
Format:	Thesis
Language:	English English
Published:	2009
Subjects:	SB183-317 Field crops Including cereals forage crops grasses legumes root crops sugar plants textile plants alkaloidal plants medicinal plants
Online Access:	https://eprints.ums.edu.my/id/eprint/38637/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/38637/2/FULLTEXT.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-ums-ep.38637
record_format	uketd_dc
spelling	my-ums-ep.386372024-05-10T02:52:46Z Isolation and characterisation of fibrolytic and lipolytic enzyme-producing bacteria from oil palm empty fruit bunch compost 2009 Elaine Remi Anak Douglas Telajan SB183-317 Field crops Including cereals, forage crops, grasses, legumes, root crops, sugar plants, textile plants, alkaloidal plants, medicinal plants Palm kernel pulp (PKC) is one of the important waste products of palm oil. PKC has been used as feed for ruminant animals and poultry and ducks. The use of PKC in general poultry and duck feed is limited due to the anti-nutritional properties such as galactomannan and xylan found in PKC. These anti-nutritional properties increase the viscosity of the diet due to high water absorption and this will limit the absorption of nutrients by farm animals. . One way to overcome this problem is to treat PKC with enzymes or microorganisms that produce enzymes that can degrade rigid components to nutritional value. Therefore, this project was implemented to isolate mesophilic and thermophilic bacteria and screen them in their ability to produce galactomannanase, cel/ulase, xylanase and lipase enzymes. These enzymes can improve the nutritional quality of PKC as a single cell protein {SCP}. Isolation is done using the 'dilution plate' method on Nutrient agar for bacteria, Starch Casein Nitrate agar {SCA) for mesophilic actinomycetes, Czapexdox Yeast Extract Casamino Acid agar {CYC) for thermophilic actinomycete, yeast extract peptone glucose agar (YEPD) for yeast and potato dextrose agar (PDA) for fungi. Commercial substrate; Azo-carob-galactomannan, Azo-xylan (oat) and AzoCM- cel/ulose were used to screen galactomannanase, xylanase and cellu/ase while sobitan monolaurate {Tween 20) was used as a substrate for lipase activity. A total of 1146 microorganisms were isolated from several sources of "empty fruit bunch" (EFB) compost, effective microbial solutions and raw EFB. Of these, 627 bacteria, 219 actinomycete, 101 yeasts and 199 fungi were isolated and screened. Six isolates with the ability to produce mannanase, ce/lulase, xylanase enzymes were quantitatively calculated mannanase, cellu/ase and xylanase activities. Found three separate Bacillus sp. different ones give mannanase activity {Isolates 7DY7, 7DU3 and 4DB3 respectively with a maximum activity of 1.30 U/μg protein, 0.95 U/μg protein and 0.92 U/μg protein), cellulase {Isolates 7DY7, 7DU3 and 4DB13 respectively with maximum activity of 0.08 U/μg protein, 0.35 U/μg protein and 0.11 U/μg protein) and xylanase (Isolate 7DY7, 7DU3 and 4D88 respectively with maximum activity of 0.15 U/μg protein, 0.08 U/μg protein and 0.21 U/μg protein) which is relatively high when compared to 13 other bacterial isolates. The six bacterial isolates were sought to be identified using their respective 165 rDNA sequences. Eleven Bacillus sp. and a single isolate of Micromonospora sp., Streptomyces sp. and Thermoactinomyces sp. each identified by comparing the partial 16S rDNA sequence of the isolates with the 16S rDNA sequence found in GenBank using the Basic Alignment Search Tool (BLAST). Microorganisms isolated can be used to treat PKC to increase the nutritional value of food in PKC 2009 Thesis https://eprints.ums.edu.my/id/eprint/38637/ https://eprints.ums.edu.my/id/eprint/38637/1/24%20PAGES.pdf text en public https://eprints.ums.edu.my/id/eprint/38637/2/FULLTEXT.pdf text en validuser masters Universiti Malaysia Sabah Institut Penyelidikan Bioteknologi
institution	Universiti Malaysia Sabah
collection	UMS Institutional Repository
language	English English
topic	SB183-317 Field crops Including cereals forage crops grasses legumes root crops sugar plants textile plants alkaloidal plants medicinal plants
spellingShingle	SB183-317 Field crops Including cereals forage crops grasses legumes root crops sugar plants textile plants alkaloidal plants medicinal plants Elaine Remi Anak Douglas Telajan Isolation and characterisation of fibrolytic and lipolytic enzyme-producing bacteria from oil palm empty fruit bunch compost
description	Palm kernel pulp (PKC) is one of the important waste products of palm oil. PKC has been used as feed for ruminant animals and poultry and ducks. The use of PKC in general poultry and duck feed is limited due to the anti-nutritional properties such as galactomannan and xylan found in PKC. These anti-nutritional properties increase the viscosity of the diet due to high water absorption and this will limit the absorption of nutrients by farm animals. . One way to overcome this problem is to treat PKC with enzymes or microorganisms that produce enzymes that can degrade rigid components to nutritional value. Therefore, this project was implemented to isolate mesophilic and thermophilic bacteria and screen them in their ability to produce galactomannanase, cel/ulase, xylanase and lipase enzymes. These enzymes can improve the nutritional quality of PKC as a single cell protein {SCP}. Isolation is done using the 'dilution plate' method on Nutrient agar for bacteria, Starch Casein Nitrate agar {SCA) for mesophilic actinomycetes, Czapexdox Yeast Extract Casamino Acid agar {CYC) for thermophilic actinomycete, yeast extract peptone glucose agar (YEPD) for yeast and potato dextrose agar (PDA) for fungi. Commercial substrate; Azo-carob-galactomannan, Azo-xylan (oat) and AzoCM- cel/ulose were used to screen galactomannanase, xylanase and cellu/ase while sobitan monolaurate {Tween 20) was used as a substrate for lipase activity. A total of 1146 microorganisms were isolated from several sources of "empty fruit bunch" (EFB) compost, effective microbial solutions and raw EFB. Of these, 627 bacteria, 219 actinomycete, 101 yeasts and 199 fungi were isolated and screened. Six isolates with the ability to produce mannanase, ce/lulase, xylanase enzymes were quantitatively calculated mannanase, cellu/ase and xylanase activities. Found three separate Bacillus sp. different ones give mannanase activity {Isolates 7DY7, 7DU3 and 4DB3 respectively with a maximum activity of 1.30 U/μg protein, 0.95 U/μg protein and 0.92 U/μg protein), cellulase {Isolates 7DY7, 7DU3 and 4DB13 respectively with maximum activity of 0.08 U/μg protein, 0.35 U/μg protein and 0.11 U/μg protein) and xylanase (Isolate 7DY7, 7DU3 and 4D88 respectively with maximum activity of 0.15 U/μg protein, 0.08 U/μg protein and 0.21 U/μg protein) which is relatively high when compared to 13 other bacterial isolates. The six bacterial isolates were sought to be identified using their respective 165 rDNA sequences. Eleven Bacillus sp. and a single isolate of Micromonospora sp., Streptomyces sp. and Thermoactinomyces sp. each identified by comparing the partial 16S rDNA sequence of the isolates with the 16S rDNA sequence found in GenBank using the Basic Alignment Search Tool (BLAST). Microorganisms isolated can be used to treat PKC to increase the nutritional value of food in PKC
format	Thesis
qualification_level	Master's degree
author	Elaine Remi Anak Douglas Telajan
author_facet	Elaine Remi Anak Douglas Telajan
author_sort	Elaine Remi Anak Douglas Telajan
title	Isolation and characterisation of fibrolytic and lipolytic enzyme-producing bacteria from oil palm empty fruit bunch compost
title_short	Isolation and characterisation of fibrolytic and lipolytic enzyme-producing bacteria from oil palm empty fruit bunch compost
title_full	Isolation and characterisation of fibrolytic and lipolytic enzyme-producing bacteria from oil palm empty fruit bunch compost
title_fullStr	Isolation and characterisation of fibrolytic and lipolytic enzyme-producing bacteria from oil palm empty fruit bunch compost
title_full_unstemmed	Isolation and characterisation of fibrolytic and lipolytic enzyme-producing bacteria from oil palm empty fruit bunch compost
title_sort	isolation and characterisation of fibrolytic and lipolytic enzyme-producing bacteria from oil palm empty fruit bunch compost
granting_institution	Universiti Malaysia Sabah
granting_department	Institut Penyelidikan Bioteknologi
publishDate	2009
url	https://eprints.ums.edu.my/id/eprint/38637/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/38637/2/FULLTEXT.pdf
_version_	1804890334881120256

Isolation and characterisation of fibrolytic and lipolytic enzyme-producing bacteria from oil palm empty fruit bunch compost

Similar Items