Antioxidative, antikinase or antiphosphatase and antimicrobial activities of selected vegetables in Sabah, Malaysia

Vegetables offer various health benefits to humans. However, there is a lack of investigation on the biological activities of these vegetables especially vegetables originating from Sabah. Thus, fifty two species of vegetable samples were collected from local markets in Sabah and were screened for t...

Full description

Saved in:

Bibliographic Details
Main Author:	Adznila Eberahim
Format:	Thesis
Language:	English English
Published:	2014
Subjects:	QK710-899 Plant physiology
Online Access:	https://eprints.ums.edu.my/id/eprint/38899/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/38899/2/FULLTEXT.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-ums-ep.38899
record_format	uketd_dc
spelling	my-ums-ep.388992024-06-21T07:07:04Z Antioxidative, antikinase or antiphosphatase and antimicrobial activities of selected vegetables in Sabah, Malaysia 2014 Adznila Eberahim QK710-899 Plant physiology Vegetables offer various health benefits to humans. However, there is a lack of investigation on the biological activities of these vegetables especially vegetables originating from Sabah. Thus, fifty two species of vegetable samples were collected from local markets in Sabah and were screened for their antioxidant activity using DPPH• assay. Five samples with high DPPH• scavenging activity ranging from 84.32% to 94.72% were selected for further study which were Cosmos caudatus, Eryngium foetidum, Ipomoea batatas, Ipomoea batatas var. green star shaped leaf and Manihot esculenta Crantz. These five samples were subjected to different solvent extractions (air-dried sample) and different cooking methods (raw sample). All of these extracts exhibits antioxidant activities ranging from 3.52% to 92.22% for all of the three antioxidant assays (DPPH•, O2• and NO• scavenging assays). Boiled water extracts of I. batatas var. green star shaped leaf and M. esculenta Crantz exhibits high antioxidant activity compared to the other solvent extractions whereas steaming and microwave cooking extracts of I. batatas var. green star shaped leaf and M. esculenta Crantz, respectively exhibits high antioxidant activity compared to the other cooking methods. Neither of the single solvent extractions or cooking methods extracts of C. caudatus and I. batatas that exhibits high antioxidant activity. In addition to that, steaming cooking extracts of E. foetidum was the only extract with high antioxidant activity. None of these extracts were exhibiting inhibition activity against GSK-3β screening test whereas there were two extracts that exhibits toxic activity against MKK1 and MSG5 screening test. These extracts were raw sample of I. batatas and I. batatas var. green star shaped leaf. Antikinase or antiphosphatase activities of these extracts were 9.33 mm (glucose media) for MKK1 whereas ranging from 7.67 mm to 8 mm (glucose media) and 8 mm to 8.33 mm (galactose media) for MSG5 screening tests. Among nine microbial strains that were tested, only two bacterial strains were inhibited by almost all of these extract which were Staphylococcus aureus and Pseudomonas aeruginosa. The antibacterial activities of these extracts were ranging from 6.67 mm to 16 mm. Raw samples of I. batatas and I. batatas var. green star shaped leaf exhibits the largest inhibition activity against S. aureus (10 mm) and P. aeruginosa (16 mm), respectively. Proximate analysis test results showed that E. foetidum has the highest moisture (87.57%) and protein contents (17.29 mg/mL) whereas M. esculenta Crantz has the highest ash content (4.11%) compared to the other vegetable species. Furthermore, I. batatas have the highest fat content (1.08%). 2014 Thesis https://eprints.ums.edu.my/id/eprint/38899/ https://eprints.ums.edu.my/id/eprint/38899/1/24%20PAGES.pdf text en public https://eprints.ums.edu.my/id/eprint/38899/2/FULLTEXT.pdf text en validuser masters Universiti Malaysia Sabah Fakulti Sains dan Sumber Alam
institution	Universiti Malaysia Sabah
collection	UMS Institutional Repository
language	English English
topic	QK710-899 Plant physiology
spellingShingle	QK710-899 Plant physiology Adznila Eberahim Antioxidative, antikinase or antiphosphatase and antimicrobial activities of selected vegetables in Sabah, Malaysia
description	Vegetables offer various health benefits to humans. However, there is a lack of investigation on the biological activities of these vegetables especially vegetables originating from Sabah. Thus, fifty two species of vegetable samples were collected from local markets in Sabah and were screened for their antioxidant activity using DPPH• assay. Five samples with high DPPH• scavenging activity ranging from 84.32% to 94.72% were selected for further study which were Cosmos caudatus, Eryngium foetidum, Ipomoea batatas, Ipomoea batatas var. green star shaped leaf and Manihot esculenta Crantz. These five samples were subjected to different solvent extractions (air-dried sample) and different cooking methods (raw sample). All of these extracts exhibits antioxidant activities ranging from 3.52% to 92.22% for all of the three antioxidant assays (DPPH•, O2• and NO• scavenging assays). Boiled water extracts of I. batatas var. green star shaped leaf and M. esculenta Crantz exhibits high antioxidant activity compared to the other solvent extractions whereas steaming and microwave cooking extracts of I. batatas var. green star shaped leaf and M. esculenta Crantz, respectively exhibits high antioxidant activity compared to the other cooking methods. Neither of the single solvent extractions or cooking methods extracts of C. caudatus and I. batatas that exhibits high antioxidant activity. In addition to that, steaming cooking extracts of E. foetidum was the only extract with high antioxidant activity. None of these extracts were exhibiting inhibition activity against GSK-3β screening test whereas there were two extracts that exhibits toxic activity against MKK1 and MSG5 screening test. These extracts were raw sample of I. batatas and I. batatas var. green star shaped leaf. Antikinase or antiphosphatase activities of these extracts were 9.33 mm (glucose media) for MKK1 whereas ranging from 7.67 mm to 8 mm (glucose media) and 8 mm to 8.33 mm (galactose media) for MSG5 screening tests. Among nine microbial strains that were tested, only two bacterial strains were inhibited by almost all of these extract which were Staphylococcus aureus and Pseudomonas aeruginosa. The antibacterial activities of these extracts were ranging from 6.67 mm to 16 mm. Raw samples of I. batatas and I. batatas var. green star shaped leaf exhibits the largest inhibition activity against S. aureus (10 mm) and P. aeruginosa (16 mm), respectively. Proximate analysis test results showed that E. foetidum has the highest moisture (87.57%) and protein contents (17.29 mg/mL) whereas M. esculenta Crantz has the highest ash content (4.11%) compared to the other vegetable species. Furthermore, I. batatas have the highest fat content (1.08%).
format	Thesis
qualification_level	Master's degree
author	Adznila Eberahim
author_facet	Adznila Eberahim
author_sort	Adznila Eberahim
title	Antioxidative, antikinase or antiphosphatase and antimicrobial activities of selected vegetables in Sabah, Malaysia
title_short	Antioxidative, antikinase or antiphosphatase and antimicrobial activities of selected vegetables in Sabah, Malaysia
title_full	Antioxidative, antikinase or antiphosphatase and antimicrobial activities of selected vegetables in Sabah, Malaysia
title_fullStr	Antioxidative, antikinase or antiphosphatase and antimicrobial activities of selected vegetables in Sabah, Malaysia
title_full_unstemmed	Antioxidative, antikinase or antiphosphatase and antimicrobial activities of selected vegetables in Sabah, Malaysia
title_sort	antioxidative, antikinase or antiphosphatase and antimicrobial activities of selected vegetables in sabah, malaysia
granting_institution	Universiti Malaysia Sabah
granting_department	Fakulti Sains dan Sumber Alam
publishDate	2014
url	https://eprints.ums.edu.my/id/eprint/38899/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/38899/2/FULLTEXT.pdf
_version_	1804890348495831040

Antioxidative, antikinase or antiphosphatase and antimicrobial activities of selected vegetables in Sabah, Malaysia

Similar Items