Chemical characteristics, antioxidant and antifungal activities of Cassia fistula Linn. at different tree ages and portions

Cassia fistula Linn. (Golden Shower Tree; Local name: Rajah Kayu) is a mediumsized tree belongs to the family Leguminosae. Its different plant portions are used in traditional treatment of various diseases and common ailments in different parts of the world since time immemorial, however still lesse...

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Main Author:	Lai, Tze Kiat
Format:	Thesis
Language:	English English
Published:	2014
Subjects:	QK710-899 Plant physiology
Online Access:	https://eprints.ums.edu.my/id/eprint/38950/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/38950/2/FULLTEXT.pdf
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spelling	my-ums-ep.389502024-06-27T07:34:00Z Chemical characteristics, antioxidant and antifungal activities of Cassia fistula Linn. at different tree ages and portions 2014 Lai, Tze Kiat QK710-899 Plant physiology Cassia fistula Linn. (Golden Shower Tree; Local name: Rajah Kayu) is a mediumsized tree belongs to the family Leguminosae. Its different plant portions are used in traditional treatment of various diseases and common ailments in different parts of the world since time immemorial, however still lesser known in Malaysia. In this project, methanolic extracts from different portions (bark, stem, leaf and root) of different age classes, namely class 1 (2-3 years), class 2 (5-10 years) and class 3 (10-15 years) of C. fistula were studied for their total extractives yield (TE) and Stiasny Number (SN) were analyzed. The antioxidant activity (AA), total phenolic content (TPC), and total tannin content (TTC) of plant extracts were analysed using 1,1-Diphenyl-2-Picryl-Hydrazyl (DPPH) Free Radical Scavenging Method and Folin- Ciocalteau Method respectively. Meanwhile, the antifungal activity (AF) of C. fistula extracts against 5 types of wood-rotting fungi, namely Pycnoporus sanguineus (PS), Microporus xanthopus (MX), Coniophora puteana (CP), Trametes spp. (T) and Stereum ostrea (SO) was determined using Poisoned Culture Medium technique. The bark portion of C. fistula produced the highest TE (3.24± 0.56%) and SN (77.77± 0.95%) compared to other portions. Meanwhile, TPC and TTC for C. fistula extract ranged from 4.58± 0.00% to 21.21± 0.05% and 1.18± 0.00% to 3.81± 0.00% respectively. The bark portion of C. fistula showed the highest AA with lowest IC50 (4.04± 0.05 mg/ml – 4.16± 0.03 mg/ml) compared to other portions. The AF showed by C. fistula extracts against wood rotting fungi was considered low and less effective. The highest inhibition activity obtained in this study was 37.75% (<50% inhibition). C. fistula extract was inactive against fungi MX. The overall study showed that, different tree portions displayed greater influences on the chemical characteristic, antioxidant and antifungal activities of C. fistula compared with different tree age classes. Bark extracts of C. fistula demonstrated a potential as natural tannin-adhesives application. The study reported that, C. fistula extracts possessed potential pharmacological properties such as AA and AF against wood rotting fungi. However, further investigation and research on AF of C. fistula against wood rotting fungi is recommended in order to identify potential specific compounds and their exact mechanism. 2014 Thesis https://eprints.ums.edu.my/id/eprint/38950/ https://eprints.ums.edu.my/id/eprint/38950/1/24%20PAGES.pdf text en public https://eprints.ums.edu.my/id/eprint/38950/2/FULLTEXT.pdf text en validuser masters Universiti Malaysia Sabah Fakulti Sains dan Sumber Alam
institution	Universiti Malaysia Sabah
collection	UMS Institutional Repository
language	English English
topic	QK710-899 Plant physiology
spellingShingle	QK710-899 Plant physiology Lai, Tze Kiat Chemical characteristics, antioxidant and antifungal activities of Cassia fistula Linn. at different tree ages and portions
description	Cassia fistula Linn. (Golden Shower Tree; Local name: Rajah Kayu) is a mediumsized tree belongs to the family Leguminosae. Its different plant portions are used in traditional treatment of various diseases and common ailments in different parts of the world since time immemorial, however still lesser known in Malaysia. In this project, methanolic extracts from different portions (bark, stem, leaf and root) of different age classes, namely class 1 (2-3 years), class 2 (5-10 years) and class 3 (10-15 years) of C. fistula were studied for their total extractives yield (TE) and Stiasny Number (SN) were analyzed. The antioxidant activity (AA), total phenolic content (TPC), and total tannin content (TTC) of plant extracts were analysed using 1,1-Diphenyl-2-Picryl-Hydrazyl (DPPH) Free Radical Scavenging Method and Folin- Ciocalteau Method respectively. Meanwhile, the antifungal activity (AF) of C. fistula extracts against 5 types of wood-rotting fungi, namely Pycnoporus sanguineus (PS), Microporus xanthopus (MX), Coniophora puteana (CP), Trametes spp. (T) and Stereum ostrea (SO) was determined using Poisoned Culture Medium technique. The bark portion of C. fistula produced the highest TE (3.24± 0.56%) and SN (77.77± 0.95%) compared to other portions. Meanwhile, TPC and TTC for C. fistula extract ranged from 4.58± 0.00% to 21.21± 0.05% and 1.18± 0.00% to 3.81± 0.00% respectively. The bark portion of C. fistula showed the highest AA with lowest IC50 (4.04± 0.05 mg/ml – 4.16± 0.03 mg/ml) compared to other portions. The AF showed by C. fistula extracts against wood rotting fungi was considered low and less effective. The highest inhibition activity obtained in this study was 37.75% (<50% inhibition). C. fistula extract was inactive against fungi MX. The overall study showed that, different tree portions displayed greater influences on the chemical characteristic, antioxidant and antifungal activities of C. fistula compared with different tree age classes. Bark extracts of C. fistula demonstrated a potential as natural tannin-adhesives application. The study reported that, C. fistula extracts possessed potential pharmacological properties such as AA and AF against wood rotting fungi. However, further investigation and research on AF of C. fistula against wood rotting fungi is recommended in order to identify potential specific compounds and their exact mechanism.
format	Thesis
qualification_level	Master's degree
author	Lai, Tze Kiat
author_facet	Lai, Tze Kiat
author_sort	Lai, Tze Kiat
title	Chemical characteristics, antioxidant and antifungal activities of Cassia fistula Linn. at different tree ages and portions
title_short	Chemical characteristics, antioxidant and antifungal activities of Cassia fistula Linn. at different tree ages and portions
title_full	Chemical characteristics, antioxidant and antifungal activities of Cassia fistula Linn. at different tree ages and portions
title_fullStr	Chemical characteristics, antioxidant and antifungal activities of Cassia fistula Linn. at different tree ages and portions
title_full_unstemmed	Chemical characteristics, antioxidant and antifungal activities of Cassia fistula Linn. at different tree ages and portions
title_sort	chemical characteristics, antioxidant and antifungal activities of cassia fistula linn. at different tree ages and portions
granting_institution	Universiti Malaysia Sabah
granting_department	Fakulti Sains dan Sumber Alam
publishDate	2014
url	https://eprints.ums.edu.my/id/eprint/38950/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/38950/2/FULLTEXT.pdf
_version_	1804890353258463232

Chemical characteristics, antioxidant and antifungal activities of Cassia fistula Linn. at different tree ages and portions

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