The prevalence of nasal colonization of Staphylococcus aureus and it's antibiotic susceptibility patterns among pre-clinical and clinical students at Universiti Malaysia Sabah

This study was a cross-sectional descriptive study to determine the prevalence of nasal colonization of Staphylococcus aureus (S. aureus) among pre-clinical and clinical medical students and nursing students attending Faculty of Medicine and Health Sciences at the Universiti Malaysia Sabah (UMS). Fr...

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Main Author:	Norfarid Irfan Mohd Subri
Format:	Thesis
Language:	English English
Published:	2017
Subjects:	RC109-216 Infectious and parasitic diseases
Online Access:	https://eprints.ums.edu.my/id/eprint/38987/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/38987/2/FULLTEXT.pdf
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spelling	my-ums-ep.389872024-06-28T07:01:33Z The prevalence of nasal colonization of Staphylococcus aureus and it's antibiotic susceptibility patterns among pre-clinical and clinical students at Universiti Malaysia Sabah 2017 Norfarid Irfan Mohd Subri RC109-216 Infectious and parasitic diseases This study was a cross-sectional descriptive study to determine the prevalence of nasal colonization of Staphylococcus aureus (S. aureus) among pre-clinical and clinical medical students and nursing students attending Faculty of Medicine and Health Sciences at the Universiti Malaysia Sabah (UMS). From April 2013 to November 2013, nasal swabs were collected from anterior nares of 449 students. Nasal swabs were inoculated on Mannitol Salt Agar (MSA) for isolation of Staphyococcus followed by Gram stain and identification of S. aureus by coagulase test using Staphylase test kit and tube coagulase test. Antibiotic susceptibility test was done on all S. aureus isolates using Kirby-Bauer method and six antibiotics discs penicillin, oxacillin, erythromycin, clindamycin, tetracycline and trimethroprim-sulfamethoxazole were included. Cefoxitin susceptibility was also performed to detect Methicillin Resistant Staphylococcus aureus (MRSA). S. aureus isolates were also tested with Slidex ® MRSA Detection kit (bioMérieux® SA) to detect Penicillin-binding protein 2a product of MRSA. The results showed that the prevalence of nasal colonization of S. aureus among 449 subjects was 31.0%, all were MSSA and none of the isolates were MRSA. Antibiotic susceptibility testing revealed that all 139 S. aureus isolates were sensitive to oxacillin, trimethroprim-sulfamethoxazole and cefoxitin, whereas 116 (83.5%), 1 (0.7%), 3 (2.2%) and 24 (17.3%) were resistant to penicillin, erythromycin, clindamycin and tetracycline respectively. This study also found out that the potential association of S. aureus colonization was not significant to all factors except the university program of students (p<0.046). In conclusion, the prevalence of S. aureus nasal colonization among pre-clinical and clinical students at UMS was 31.0%. No MRSA was detected and the S. aureus isolates were highly resistant to penicillin. 2017 Thesis https://eprints.ums.edu.my/id/eprint/38987/ https://eprints.ums.edu.my/id/eprint/38987/1/24%20PAGES.pdf text en public https://eprints.ums.edu.my/id/eprint/38987/2/FULLTEXT.pdf text en validuser masters Universiti Malaysia Sabah Fakulti Perubatan dan Sains Kesihatan
institution	Universiti Malaysia Sabah
collection	UMS Institutional Repository
language	English English
topic	RC109-216 Infectious and parasitic diseases
spellingShingle	RC109-216 Infectious and parasitic diseases Norfarid Irfan Mohd Subri The prevalence of nasal colonization of Staphylococcus aureus and it's antibiotic susceptibility patterns among pre-clinical and clinical students at Universiti Malaysia Sabah
description	This study was a cross-sectional descriptive study to determine the prevalence of nasal colonization of Staphylococcus aureus (S. aureus) among pre-clinical and clinical medical students and nursing students attending Faculty of Medicine and Health Sciences at the Universiti Malaysia Sabah (UMS). From April 2013 to November 2013, nasal swabs were collected from anterior nares of 449 students. Nasal swabs were inoculated on Mannitol Salt Agar (MSA) for isolation of Staphyococcus followed by Gram stain and identification of S. aureus by coagulase test using Staphylase test kit and tube coagulase test. Antibiotic susceptibility test was done on all S. aureus isolates using Kirby-Bauer method and six antibiotics discs penicillin, oxacillin, erythromycin, clindamycin, tetracycline and trimethroprim-sulfamethoxazole were included. Cefoxitin susceptibility was also performed to detect Methicillin Resistant Staphylococcus aureus (MRSA). S. aureus isolates were also tested with Slidex ® MRSA Detection kit (bioMérieux® SA) to detect Penicillin-binding protein 2a product of MRSA. The results showed that the prevalence of nasal colonization of S. aureus among 449 subjects was 31.0%, all were MSSA and none of the isolates were MRSA. Antibiotic susceptibility testing revealed that all 139 S. aureus isolates were sensitive to oxacillin, trimethroprim-sulfamethoxazole and cefoxitin, whereas 116 (83.5%), 1 (0.7%), 3 (2.2%) and 24 (17.3%) were resistant to penicillin, erythromycin, clindamycin and tetracycline respectively. This study also found out that the potential association of S. aureus colonization was not significant to all factors except the university program of students (p<0.046). In conclusion, the prevalence of S. aureus nasal colonization among pre-clinical and clinical students at UMS was 31.0%. No MRSA was detected and the S. aureus isolates were highly resistant to penicillin.
format	Thesis
qualification_level	Master's degree
author	Norfarid Irfan Mohd Subri
author_facet	Norfarid Irfan Mohd Subri
author_sort	Norfarid Irfan Mohd Subri
title	The prevalence of nasal colonization of Staphylococcus aureus and it's antibiotic susceptibility patterns among pre-clinical and clinical students at Universiti Malaysia Sabah
title_short	The prevalence of nasal colonization of Staphylococcus aureus and it's antibiotic susceptibility patterns among pre-clinical and clinical students at Universiti Malaysia Sabah
title_full	The prevalence of nasal colonization of Staphylococcus aureus and it's antibiotic susceptibility patterns among pre-clinical and clinical students at Universiti Malaysia Sabah
title_fullStr	The prevalence of nasal colonization of Staphylococcus aureus and it's antibiotic susceptibility patterns among pre-clinical and clinical students at Universiti Malaysia Sabah
title_full_unstemmed	The prevalence of nasal colonization of Staphylococcus aureus and it's antibiotic susceptibility patterns among pre-clinical and clinical students at Universiti Malaysia Sabah
title_sort	prevalence of nasal colonization of staphylococcus aureus and it's antibiotic susceptibility patterns among pre-clinical and clinical students at universiti malaysia sabah
granting_institution	Universiti Malaysia Sabah
granting_department	Fakulti Perubatan dan Sains Kesihatan
publishDate	2017
url	https://eprints.ums.edu.my/id/eprint/38987/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/38987/2/FULLTEXT.pdf
_version_	1804890359790043136

The prevalence of nasal colonization of Staphylococcus aureus and it's antibiotic susceptibility patterns among pre-clinical and clinical students at Universiti Malaysia Sabah

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