Correlation of oxidative stress with serum essential elements level in beta thalassaemia major patients in Sabah

Beta thalassaemia major is one of the leading public health problems in Malaysia affecting significant number of infants born each year. It is the severe form of beta thalassaemia resulting from homozygosity for the phenotype β0 (β0β0). Although repeated blood transfusions are obligatory for the sur...

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Main Author:	Shazia Qaiser
Format:	Thesis
Language:	English English
Published:	2015
Subjects:	RC633-647.5 Diseases of the blood and blood-forming organs
Online Access:	https://eprints.ums.edu.my/id/eprint/40612/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/40612/2/FULLTEXT.pdf
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spelling	my-ums-ep.406122024-08-21T08:40:18Z Correlation of oxidative stress with serum essential elements level in beta thalassaemia major patients in Sabah 2015 Shazia Qaiser RC633-647.5 Diseases of the blood and blood-forming organs Beta thalassaemia major is one of the leading public health problems in Malaysia affecting significant number of infants born each year. It is the severe form of beta thalassaemia resulting from homozygosity for the phenotype β0 (β0β0). Although repeated blood transfusions are obligatory for the survival of beta thalassaemia major patients, it also accelerates oxidative stress secondary to iron overload. This study aims to determine the correlation between oxidative stress and serum essential elements level in beta thalassemia major patients. One hundred randomly selected transfusion dependent beta thalassaemia major patients on chelation therapy and one hundred non thalassaemic healthy controls were included in this study. Essential elements were estimated by using Flame atomic absorption spectrophotometer (FAAS) while oxidative stress markers were determined using dual beam spectrophotometer. Statistical analysis of the data was performed using independent t-test and Pearson’s correlation analysis. The p-value < 0.05 is considered statistically significant in this research. In the present study, significantly decreased (p< 0.05) serum concentrations of zinc (Zn) and magnesium (Mg) while significantly increased (p< 0.05) serum concentrations of manganese (Mn) and chromium (Cr) were found in beta thalassaemia major patients as compared to the controls. The serum levels of copper (Cu) and potassium (K) remain unchanged. In addition, the serum levels of reduced glutathione (GSH) were significantly lower (p< 0.05) while serum levels of glutathione s transferase (GST), catalase (CAT) and lipid peroxidation products (LPO) were significantly higher (p< 0.01) in beta thalassaemia major patients as compared to the healthy counterparts. Interestingly, the age and gender based alteration in levels of serum Mn and CAT activity was also observed. Furthermore the results from the Pearson’s correlation suggest that a significant negative correlation present between serum LPO levels and serum Zn and Mg levels. Moreover, a significant positive correlation was found between serum Mn and serum GST levels in beta thalassaemia major patients. In conclusion, the presence of a correlation between oxidative stress and essential elements was observed in beta thalassaemia major patients. The findings of this study suggest that increased oxidative stress may alter the level of certain essential elements in beta thalassaemia major patients. 2015 Thesis https://eprints.ums.edu.my/id/eprint/40612/ https://eprints.ums.edu.my/id/eprint/40612/1/24%20PAGES.pdf text en public https://eprints.ums.edu.my/id/eprint/40612/2/FULLTEXT.pdf text en validuser masters Universiti Malaysia Sabah Fakulti Perubatan dan Sains Kesihatan
institution	Universiti Malaysia Sabah
collection	UMS Institutional Repository
language	English English
topic	RC633-647.5 Diseases of the blood and blood-forming organs
spellingShingle	RC633-647.5 Diseases of the blood and blood-forming organs Shazia Qaiser Correlation of oxidative stress with serum essential elements level in beta thalassaemia major patients in Sabah
description	Beta thalassaemia major is one of the leading public health problems in Malaysia affecting significant number of infants born each year. It is the severe form of beta thalassaemia resulting from homozygosity for the phenotype β0 (β0β0). Although repeated blood transfusions are obligatory for the survival of beta thalassaemia major patients, it also accelerates oxidative stress secondary to iron overload. This study aims to determine the correlation between oxidative stress and serum essential elements level in beta thalassemia major patients. One hundred randomly selected transfusion dependent beta thalassaemia major patients on chelation therapy and one hundred non thalassaemic healthy controls were included in this study. Essential elements were estimated by using Flame atomic absorption spectrophotometer (FAAS) while oxidative stress markers were determined using dual beam spectrophotometer. Statistical analysis of the data was performed using independent t-test and Pearson’s correlation analysis. The p-value < 0.05 is considered statistically significant in this research. In the present study, significantly decreased (p< 0.05) serum concentrations of zinc (Zn) and magnesium (Mg) while significantly increased (p< 0.05) serum concentrations of manganese (Mn) and chromium (Cr) were found in beta thalassaemia major patients as compared to the controls. The serum levels of copper (Cu) and potassium (K) remain unchanged. In addition, the serum levels of reduced glutathione (GSH) were significantly lower (p< 0.05) while serum levels of glutathione s transferase (GST), catalase (CAT) and lipid peroxidation products (LPO) were significantly higher (p< 0.01) in beta thalassaemia major patients as compared to the healthy counterparts. Interestingly, the age and gender based alteration in levels of serum Mn and CAT activity was also observed. Furthermore the results from the Pearson’s correlation suggest that a significant negative correlation present between serum LPO levels and serum Zn and Mg levels. Moreover, a significant positive correlation was found between serum Mn and serum GST levels in beta thalassaemia major patients. In conclusion, the presence of a correlation between oxidative stress and essential elements was observed in beta thalassaemia major patients. The findings of this study suggest that increased oxidative stress may alter the level of certain essential elements in beta thalassaemia major patients.
format	Thesis
qualification_level	Master's degree
author	Shazia Qaiser
author_facet	Shazia Qaiser
author_sort	Shazia Qaiser
title	Correlation of oxidative stress with serum essential elements level in beta thalassaemia major patients in Sabah
title_short	Correlation of oxidative stress with serum essential elements level in beta thalassaemia major patients in Sabah
title_full	Correlation of oxidative stress with serum essential elements level in beta thalassaemia major patients in Sabah
title_fullStr	Correlation of oxidative stress with serum essential elements level in beta thalassaemia major patients in Sabah
title_full_unstemmed	Correlation of oxidative stress with serum essential elements level in beta thalassaemia major patients in Sabah
title_sort	correlation of oxidative stress with serum essential elements level in beta thalassaemia major patients in sabah
granting_institution	Universiti Malaysia Sabah
granting_department	Fakulti Perubatan dan Sains Kesihatan
publishDate	2015
url	https://eprints.ums.edu.my/id/eprint/40612/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/40612/2/FULLTEXT.pdf
_version_	1811770538421387264

Correlation of oxidative stress with serum essential elements level in beta thalassaemia major patients in Sabah

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