Antioxidative potential of selected edible wild mushrooms from Sabah

The objectives of the present study were to evaluate the in vitro antioxidant properties of four species of edible wild mushrooms namely, Pleurotus porrigens, Schizophyllum commune, Hygrocybe conica, and Lentinus ciliatus, optimization of the extraction conditions for antioxidant activity of the mus...

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Main Author:	Yim, Hip Seng
Format:	Thesis
Language:	English English
Published:	2013
Subjects:	TX341-641 Nutrition Foods and food supply
Online Access:	https://eprints.ums.edu.my/id/eprint/41821/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/41821/2/FULLTEXT.pdf
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spelling	my-ums-ep.418212024-12-04T07:23:37Z Antioxidative potential of selected edible wild mushrooms from Sabah 2013 Yim, Hip Seng TX341-641 Nutrition. Foods and food supply The objectives of the present study were to evaluate the in vitro antioxidant properties of four species of edible wild mushrooms namely, Pleurotus porrigens, Schizophyllum commune, Hygrocybe conica, and Lentinus ciliatus, optimization of the extraction conditions for antioxidant activity of the mushrooms; identification of the potent antioxidative components from the selected mushrooms; and the evaluation of the oxidative stability of cooking oil supplemented with selected mushroom extracts. The antioxidant properties of edible wild mushrooms were evaluated using 2,2-diphenyl-1-picrylhydrazyl (OPPH) radical scavenging ability, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation inhibition, ferric reducing antioxidant power (FRAP), and ß-carotene-linoleate bleaching (ß-CB). Total phenolic content (TPC) was determined using Folin-Ciocalteu's method. Response surface methodology (RSM) was employed to optimize the extraction time and temperature of P. porrigens and S. commune for the maximal yields of antioxidant activity. Fractionation by means of column chromatography was carried out and the identification of major anti oxidative components was performed by liquid chromatography-mass spectrometry analysis. The oxidative stability of sunflower oil was determined by peroxide value, iodine value, p-anisidine value, and thiobarbituric acid-reactive substances. Results showed that P. porrigens had significantly higher (p < 0.05) DPPH radical scavenging ability and FRAP than that of butylated hydroxyanisole (BHA), while S. commune showed comparable DPPH radical scavenging ability and ABTS radical cation inhibition with BHA. Total phenolic content was found in a descending order of P. porrigens > L. Ciliatus = P. ostreatus > H. conica = S. commune. Positive correlations were found between TPC and DPPH; ABTS; FRAP; and ß-CB, respectively, indicating that the presence of phenolic compounds contribute to the antioxidant activities of the edible wild mushrooms. The optimized extraction time and temperature of P. porrigens and S. commune were 315.5 min and 37.4°C; and 213.2 min and 41.5°C, respectively. The values obtained experimentally agreed well with the predicted values, indicating the suitability of respective RSM models for maximal yields of antioxidant activity. Both sub-fraction (SF)-III of P. porrigens and S. commune showed consistently higher DPPH radical scavenging ability, FRAP, and TPC with two flavones glucosides, namely luteolin 7-O-ß-glucoside and apigenin 7-O-ß-glucoside were identified in both SF-III. The sunflower oils supplemented with P. porrigens and S. commune extracts were found to be able to prolong the shelf-life between 1 and 2 years by retarding the formations of primary and secondary oxidation products, and reducing losses of polyunsaturated fatty acids. In conclusion, the selected edible wild mushrooms showed promising antioxidant activity, and luteolin 7-O-ß-glucoside and apigenin 7-O-ß-glucoside were identified in SF-III of P. porrigens and S. commune. Thus, edible wild mushrooms from Sabah can be promoted as antioxidant-rich foods as well as potential sources of natural antioxidants for food industry application. 2013 Thesis https://eprints.ums.edu.my/id/eprint/41821/ https://eprints.ums.edu.my/id/eprint/41821/1/24%20PAGES.pdf text en public https://eprints.ums.edu.my/id/eprint/41821/2/FULLTEXT.pdf text en validuser dphil doctoral Universiti Malaysia Sabah Sekolah Sains Makanan dan Pemakanan
institution	Universiti Malaysia Sabah
collection	UMS Institutional Repository
language	English English
topic	TX341-641 Nutrition Foods and food supply
spellingShingle	TX341-641 Nutrition Foods and food supply Yim, Hip Seng Antioxidative potential of selected edible wild mushrooms from Sabah
description	The objectives of the present study were to evaluate the in vitro antioxidant properties of four species of edible wild mushrooms namely, Pleurotus porrigens, Schizophyllum commune, Hygrocybe conica, and Lentinus ciliatus, optimization of the extraction conditions for antioxidant activity of the mushrooms; identification of the potent antioxidative components from the selected mushrooms; and the evaluation of the oxidative stability of cooking oil supplemented with selected mushroom extracts. The antioxidant properties of edible wild mushrooms were evaluated using 2,2-diphenyl-1-picrylhydrazyl (OPPH) radical scavenging ability, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation inhibition, ferric reducing antioxidant power (FRAP), and ß-carotene-linoleate bleaching (ß-CB). Total phenolic content (TPC) was determined using Folin-Ciocalteu's method. Response surface methodology (RSM) was employed to optimize the extraction time and temperature of P. porrigens and S. commune for the maximal yields of antioxidant activity. Fractionation by means of column chromatography was carried out and the identification of major anti oxidative components was performed by liquid chromatography-mass spectrometry analysis. The oxidative stability of sunflower oil was determined by peroxide value, iodine value, p-anisidine value, and thiobarbituric acid-reactive substances. Results showed that P. porrigens had significantly higher (p < 0.05) DPPH radical scavenging ability and FRAP than that of butylated hydroxyanisole (BHA), while S. commune showed comparable DPPH radical scavenging ability and ABTS radical cation inhibition with BHA. Total phenolic content was found in a descending order of P. porrigens > L. Ciliatus = P. ostreatus > H. conica = S. commune. Positive correlations were found between TPC and DPPH; ABTS; FRAP; and ß-CB, respectively, indicating that the presence of phenolic compounds contribute to the antioxidant activities of the edible wild mushrooms. The optimized extraction time and temperature of P. porrigens and S. commune were 315.5 min and 37.4°C; and 213.2 min and 41.5°C, respectively. The values obtained experimentally agreed well with the predicted values, indicating the suitability of respective RSM models for maximal yields of antioxidant activity. Both sub-fraction (SF)-III of P. porrigens and S. commune showed consistently higher DPPH radical scavenging ability, FRAP, and TPC with two flavones glucosides, namely luteolin 7-O-ß-glucoside and apigenin 7-O-ß-glucoside were identified in both SF-III. The sunflower oils supplemented with P. porrigens and S. commune extracts were found to be able to prolong the shelf-life between 1 and 2 years by retarding the formations of primary and secondary oxidation products, and reducing losses of polyunsaturated fatty acids. In conclusion, the selected edible wild mushrooms showed promising antioxidant activity, and luteolin 7-O-ß-glucoside and apigenin 7-O-ß-glucoside were identified in SF-III of P. porrigens and S. commune. Thus, edible wild mushrooms from Sabah can be promoted as antioxidant-rich foods as well as potential sources of natural antioxidants for food industry application.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Yim, Hip Seng
author_facet	Yim, Hip Seng
author_sort	Yim, Hip Seng
title	Antioxidative potential of selected edible wild mushrooms from Sabah
title_short	Antioxidative potential of selected edible wild mushrooms from Sabah
title_full	Antioxidative potential of selected edible wild mushrooms from Sabah
title_fullStr	Antioxidative potential of selected edible wild mushrooms from Sabah
title_full_unstemmed	Antioxidative potential of selected edible wild mushrooms from Sabah
title_sort	antioxidative potential of selected edible wild mushrooms from sabah
granting_institution	Universiti Malaysia Sabah
granting_department	Sekolah Sains Makanan dan Pemakanan
publishDate	2013
url	https://eprints.ums.edu.my/id/eprint/41821/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/41821/2/FULLTEXT.pdf
_version_	1818611432620556288

Antioxidative potential of selected edible wild mushrooms from Sabah

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