Molecular epidemiology of malaria in the interior division of Sabah, Malaysia

Malaria is one of the important parasite transmitted diseases in Sabah, Malaysian Borneo that is covered by tropical rainforest. The objectives of this six months cross-sectional study were to accurately identify the human malaria parasites, to determine the rate of misidentification and to characte...

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Main Author:	Neoh, Joveen Wan Fen
Format:	Thesis
Language:	English
Published:	2011
Subjects:	RA Public aspects of medicine
Online Access:	https://eprints.ums.edu.my/id/eprint/6667/1/mt0000000210.pdf
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spelling	my-ums-ep.66672017-10-11T07:57:25Z Molecular epidemiology of malaria in the interior division of Sabah, Malaysia 2011 Neoh, Joveen Wan Fen RA Public aspects of medicine Malaria is one of the important parasite transmitted diseases in Sabah, Malaysian Borneo that is covered by tropical rainforest. The objectives of this six months cross-sectional study were to accurately identify the human malaria parasites, to determine the rate of misidentification and to characterize P. knowlesi isolates in the interior division of Sabah based on ssrRNA gene using nested PCR. A total of 243 blood spot samples from patients who had requested for Blood Film for Malaria Parasite (BFMP) test were collected from four study sites namely Keningau, Tenom, Tambunan and Nabawan. There were 16.1% P. malariae, 10.3% P. vivax, 7% P. falciparum, 1.2% mixed infection and 65.4% negative cases based on microscopic examination. However, the result of PCR indicated that P. knowlesi (58.9%), P. falciparum (18.7%), P. vivax (18.7%), mixed infection (3.7%) and no P. malariae infection were detected in these samples. There were only 35% of 243 samples gave consistent PCR and microscopic results. The highest malaria cases found in Keningau were P. falciparum (36.1%) whereas the prevalence of P. knowlesi was higher in Tambunan (85.7%) and Tenom (77.8%). Moreover, six positive samples for P. falciparum and P. knowlesi each were detected in Nabawan. Alignment analysis between P. knowlesi isolates from this study and P. knowlesi isolates from other geographical region showed two single nucleotide polymorphisms unique to P. knowlesi isolates from the interior division of Sabah. Besides, isolate KN048/2010 showing significant genetic variation among P. knowlesi isolates from this region. Phylogenetic analysis showed that P. knowlesi from this study clustered with naturally-acquired P. knowlesiisolates in human. This study provides further evidence of the actual transmission of different Plasmodium species in the interior regions of Sabah. 2011 Thesis https://eprints.ums.edu.my/id/eprint/6667/ https://eprints.ums.edu.my/id/eprint/6667/1/mt0000000210.pdf text en public masters Universiti Malaysia Sabah Biotechnology Research Institute (BRI)
institution	Universiti Malaysia Sabah
collection	UMS Institutional Repository
language	English
topic	RA Public aspects of medicine
spellingShingle	RA Public aspects of medicine Neoh, Joveen Wan Fen Molecular epidemiology of malaria in the interior division of Sabah, Malaysia
description	Malaria is one of the important parasite transmitted diseases in Sabah, Malaysian Borneo that is covered by tropical rainforest. The objectives of this six months cross-sectional study were to accurately identify the human malaria parasites, to determine the rate of misidentification and to characterize P. knowlesi isolates in the interior division of Sabah based on ssrRNA gene using nested PCR. A total of 243 blood spot samples from patients who had requested for Blood Film for Malaria Parasite (BFMP) test were collected from four study sites namely Keningau, Tenom, Tambunan and Nabawan. There were 16.1% P. malariae, 10.3% P. vivax, 7% P. falciparum, 1.2% mixed infection and 65.4% negative cases based on microscopic examination. However, the result of PCR indicated that P. knowlesi (58.9%), P. falciparum (18.7%), P. vivax (18.7%), mixed infection (3.7%) and no P. malariae infection were detected in these samples. There were only 35% of 243 samples gave consistent PCR and microscopic results. The highest malaria cases found in Keningau were P. falciparum (36.1%) whereas the prevalence of P. knowlesi was higher in Tambunan (85.7%) and Tenom (77.8%). Moreover, six positive samples for P. falciparum and P. knowlesi each were detected in Nabawan. Alignment analysis between P. knowlesi isolates from this study and P. knowlesi isolates from other geographical region showed two single nucleotide polymorphisms unique to P. knowlesi isolates from the interior division of Sabah. Besides, isolate KN048/2010 showing significant genetic variation among P. knowlesi isolates from this region. Phylogenetic analysis showed that P. knowlesi from this study clustered with naturally-acquired P. knowlesiisolates in human. This study provides further evidence of the actual transmission of different Plasmodium species in the interior regions of Sabah.
format	Thesis
qualification_level	Master's degree
author	Neoh, Joveen Wan Fen
author_facet	Neoh, Joveen Wan Fen
author_sort	Neoh, Joveen Wan Fen
title	Molecular epidemiology of malaria in the interior division of Sabah, Malaysia
title_short	Molecular epidemiology of malaria in the interior division of Sabah, Malaysia
title_full	Molecular epidemiology of malaria in the interior division of Sabah, Malaysia
title_fullStr	Molecular epidemiology of malaria in the interior division of Sabah, Malaysia
title_full_unstemmed	Molecular epidemiology of malaria in the interior division of Sabah, Malaysia
title_sort	molecular epidemiology of malaria in the interior division of sabah, malaysia
granting_institution	Universiti Malaysia Sabah
granting_department	Biotechnology Research Institute (BRI)
publishDate	2011
url	https://eprints.ums.edu.my/id/eprint/6667/1/mt0000000210.pdf
_version_	1747836328139030528

Molecular epidemiology of malaria in the interior division of Sabah, Malaysia

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