Characterization of a MADS-box gene from Musa sp. (pisang berangan)

Homeotic genes are known to play important roles in the development of fruits and flowers. Most of these genes belong to a large family of regulatory genes that have a characteristic DNA binding domain known as the MADS-box. As a pioneer effort in the study of homeotic genes from banana, a full leng...

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Main Author:	Jagdish Kaur Chahil Santokh Singh
Format:	Thesis
Language:	English English
Published:	2008
Subjects:	SB354-402 Fruit and fruit culture
Online Access:	https://eprints.ums.edu.my/id/eprint/8990/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/8990/2/FULLTEXT.pdf
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id	my-ums-ep.8990
record_format	uketd_dc
spelling	my-ums-ep.89902024-02-08T01:59:14Z Characterization of a MADS-box gene from Musa sp. (pisang berangan) 2008 Jagdish Kaur Chahil Santokh Singh SB354-402 Fruit and fruit culture Homeotic genes are known to play important roles in the development of fruits and flowers. Most of these genes belong to a large family of regulatory genes that have a characteristic DNA binding domain known as the MADS-box. As a pioneer effort in the study of homeotic genes from banana, a full length MADS-box cDNA from an inflorescence of Musa acuminata var. Berangan designated as MADS3 has been successfully isolated and characterized. The approach of this research was by isolating Poly A+ mRNA from the inflorescence of Pisang Berangan using oligo-dT magnetic beads. First strand cDNA was synthesized using a dT18 anchored primer directly onto those beads. A degenerate primer designed by aligning the sequences of the 180bp MADS domain from known MADS-box genes was used as the forward primer to perform a 3' RACE. This generated a complete coding sequence. To obtain the 5' untranslated region on the N terminal side of the MADS-box domain a 5' RACE was carried out. NCBI BLAST analyses were done on these sequences to confirm authenticity to known MADS-box genes. The PHYLIP package was used to further analyze these sequences. The putative 244 amino acid sequence deduced from this research supporting the MADS-box region and the K domain of the corresponding gene suggests that it is a member of the Type II family. MADS3 belongs to the AGL clade and is a member of the SEPALLATA subfamily of MADS-box transcription factors. 2008 Thesis https://eprints.ums.edu.my/id/eprint/8990/ https://eprints.ums.edu.my/id/eprint/8990/1/24%20PAGES.pdf text en public https://eprints.ums.edu.my/id/eprint/8990/2/FULLTEXT.pdf text en validuser masters University Malaysia Sabah School of Science and Technology
institution	Universiti Malaysia Sabah
collection	UMS Institutional Repository
language	English English
topic	SB354-402 Fruit and fruit culture
spellingShingle	SB354-402 Fruit and fruit culture Jagdish Kaur Chahil Santokh Singh Characterization of a MADS-box gene from Musa sp. (pisang berangan)
description	Homeotic genes are known to play important roles in the development of fruits and flowers. Most of these genes belong to a large family of regulatory genes that have a characteristic DNA binding domain known as the MADS-box. As a pioneer effort in the study of homeotic genes from banana, a full length MADS-box cDNA from an inflorescence of Musa acuminata var. Berangan designated as MADS3 has been successfully isolated and characterized. The approach of this research was by isolating Poly A+ mRNA from the inflorescence of Pisang Berangan using oligo-dT magnetic beads. First strand cDNA was synthesized using a dT18 anchored primer directly onto those beads. A degenerate primer designed by aligning the sequences of the 180bp MADS domain from known MADS-box genes was used as the forward primer to perform a 3' RACE. This generated a complete coding sequence. To obtain the 5' untranslated region on the N terminal side of the MADS-box domain a 5' RACE was carried out. NCBI BLAST analyses were done on these sequences to confirm authenticity to known MADS-box genes. The PHYLIP package was used to further analyze these sequences. The putative 244 amino acid sequence deduced from this research supporting the MADS-box region and the K domain of the corresponding gene suggests that it is a member of the Type II family. MADS3 belongs to the AGL clade and is a member of the SEPALLATA subfamily of MADS-box transcription factors.
format	Thesis
qualification_level	Master's degree
author	Jagdish Kaur Chahil Santokh Singh
author_facet	Jagdish Kaur Chahil Santokh Singh
author_sort	Jagdish Kaur Chahil Santokh Singh
title	Characterization of a MADS-box gene from Musa sp. (pisang berangan)
title_short	Characterization of a MADS-box gene from Musa sp. (pisang berangan)
title_full	Characterization of a MADS-box gene from Musa sp. (pisang berangan)
title_fullStr	Characterization of a MADS-box gene from Musa sp. (pisang berangan)
title_full_unstemmed	Characterization of a MADS-box gene from Musa sp. (pisang berangan)
title_sort	characterization of a mads-box gene from musa sp. (pisang berangan)
granting_institution	University Malaysia Sabah
granting_department	School of Science and Technology
publishDate	2008
url	https://eprints.ums.edu.my/id/eprint/8990/1/24%20PAGES.pdf https://eprints.ums.edu.my/id/eprint/8990/2/FULLTEXT.pdf
_version_	1794022693961465856

Characterization of a MADS-box gene from Musa sp. (pisang berangan)

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