The role of cyclic nucleotides in the model plant arabidopsis

Adenylyl cyclases (ACs) catalyze the formation of adenosine3',5'-cyclic monophosphate (cAMP) from adenosine 5'-triphosphate (ATP). The Arabidopsis genome contains two genes, At3g14460 (AC1) and At3g14470 (AC2) which have an aminoterminal nucleotide binding domain and sequence homolo...

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Main Author:	Malinna Jusoh
Format:	Thesis
Language:	English
Subjects:	QP 625 .N89 M3 2010 Malinna Jusoh The role of cyclic nucleotides in the model plant arabidopsis Tesis University of Oxford 2010 Cyclic nucleotides
Online Access:	http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/1011/4/QP%20625%20.N89%20M3%202010%20Abstract.pdf http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/1011/5/QP%20625%20.N89%20M3%202010%20FullText.pdf
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spelling	my-umt-ir.-10112016-03-11T22:32:58Z The role of cyclic nucleotides in the model plant arabidopsis Malinna Jusoh Adenylyl cyclases (ACs) catalyze the formation of adenosine3',5'-cyclic monophosphate (cAMP) from adenosine 5'-triphosphate (ATP). The Arabidopsis genome contains two genes, At3g14460 (AC1) and At3g14470 (AC2) which have an aminoterminal nucleotide binding domain and sequence homology to maize AC. Expression of AC1 and AC2 complemented the E.coli cAMP mutant, cya suggesting that both proteins produce functional cAMP in vivo. Gene expression studies using RT-PCR and promoter- GUS staining showed that AC1 is expressed in root tips, inflorescence and anthers, while AC2 is expressed in stem, roots, cauline leaves, senescent leaves, and inflorescences. TDNA insertion mutants of ac1 and ac2 showed a delay and a reduction in germination under salt stress. Conversely, overexpression of AC1 and AC2 conferred some protection against salt stress. Taken together, I infer that cAMP may have a role in salinity tolerance. University of Oxford 2010 Thesis en http://hdl.handle.net/123456789/1011 http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/1011/3/license.txt ecca7a0b60eabbf88d99eae3662e2d50 http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/1011/4/QP%20625%20.N89%20M3%202010%20Abstract.pdf 72f3f1f22a384ff055c70caf4a06719b http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/1011/5/QP%20625%20.N89%20M3%202010%20FullText.pdf 9aae8f019656805ba583e7fde3f362b1 http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/1011/6/QP%20625%20.N89%20M3%202010%20Abstract.pdf.txt 94b70a33afcedc26016d13998ab57a67 http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/1011/7/QP%20625%20.N89%20M3%202010%20FullText.pdf.txt 41e032d23569156f72c797c61f55a367 QP 625 .N89 M3 2010 Malinna Jusoh The role of cyclic nucleotides in the model plant arabidopsis Tesis University of Oxford 2010 Cyclic nucleotides
institution	Universiti Malaysia Terengganu
collection	UMT Repository System
language	English
topic	QP 625 .N89 M3 2010 Malinna Jusoh The role of cyclic nucleotides in the model plant arabidopsis Tesis University of Oxford 2010 Cyclic nucleotides
spellingShingle	QP 625 .N89 M3 2010 Malinna Jusoh The role of cyclic nucleotides in the model plant arabidopsis Tesis University of Oxford 2010 Cyclic nucleotides Malinna Jusoh The role of cyclic nucleotides in the model plant arabidopsis
description	Adenylyl cyclases (ACs) catalyze the formation of adenosine3',5'-cyclic monophosphate (cAMP) from adenosine 5'-triphosphate (ATP). The Arabidopsis genome contains two genes, At3g14460 (AC1) and At3g14470 (AC2) which have an aminoterminal nucleotide binding domain and sequence homology to maize AC. Expression of AC1 and AC2 complemented the E.coli cAMP mutant, cya suggesting that both proteins produce functional cAMP in vivo. Gene expression studies using RT-PCR and promoter- GUS staining showed that AC1 is expressed in root tips, inflorescence and anthers, while AC2 is expressed in stem, roots, cauline leaves, senescent leaves, and inflorescences. TDNA insertion mutants of ac1 and ac2 showed a delay and a reduction in germination under salt stress. Conversely, overexpression of AC1 and AC2 conferred some protection against salt stress. Taken together, I infer that cAMP may have a role in salinity tolerance.
format	Thesis
author	Malinna Jusoh
author_facet	Malinna Jusoh
author_sort	Malinna Jusoh
title	The role of cyclic nucleotides in the model plant arabidopsis
title_short	The role of cyclic nucleotides in the model plant arabidopsis
title_full	The role of cyclic nucleotides in the model plant arabidopsis
title_fullStr	The role of cyclic nucleotides in the model plant arabidopsis
title_full_unstemmed	The role of cyclic nucleotides in the model plant arabidopsis
title_sort	role of cyclic nucleotides in the model plant arabidopsis
granting_institution	University of Oxford
url	http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/1011/4/QP%20625%20.N89%20M3%202010%20Abstract.pdf http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/1011/5/QP%20625%20.N89%20M3%202010%20FullText.pdf
_version_	1747835812921212928

The role of cyclic nucleotides in the model plant arabidopsis

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