OPTIMIZATION OF PROTEASE FROM ASPERGILLUS NIGER IN SOLID STATE FERMENTATION FOR ENZYMATIC TREATMENT OF AQUACULTURE SLUDGE

Proteases or proteolytic enzymes, has high potential to be applied in wastewater treatment and improve sludge dewatering. Hence, the ultimate aim of this study is to produce and characterize protease from Aspergillus niger under solid-state fermentation (SSF) by utilizing shrimp shell powder as s...

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Main Author:	OOI CHEE KUAN
Format:	Thesis
Language:	English
Online Access:	http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/16179/1/Abstract.pdf http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/16179/2/Full%20Thesis%20-%20OOI%20CHEE%20KUAN.pdf
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id	my-umt-ir.-16179
record_format	uketd_dc
spelling	my-umt-ir.-161792022-02-07T03:39:20Z OPTIMIZATION OF PROTEASE FROM ASPERGILLUS NIGER IN SOLID STATE FERMENTATION FOR ENZYMATIC TREATMENT OF AQUACULTURE SLUDGE OOI CHEE KUAN Proteases or proteolytic enzymes, has high potential to be applied in wastewater treatment and improve sludge dewatering. Hence, the ultimate aim of this study is to produce and characterize protease from Aspergillus niger under solid-state fermentation (SSF) by utilizing shrimp shell powder as solid substrate. The produced protease was applied for enzymatic treatment to reduce organic matter and micropollutants in aquaculture sludge based on selected parameters. It was found that the produced protease from SSF was slightly alkaline. The correlation between factors operating parameters (incubation temperature, inoculum size, moisture content) for the production of enzyme are analysed using statistical software, Minitab 16. A 23 full factorial experimental design was employed and the enzyme produced was optimized by the method of desirability function. The optimal conditions for protease production of 3.7 U/mg were 35 °C of incubation temperature, 60% of initial moisture content and 1.0 of inoculum size. It was found that aquaculture sludge collected contained high total suspended solids (TSS) and chemical oxygen demand (COD), which were 8280 mg/L and 5866.67 mg/L respectively. SSF protease and commercial protease was applied for enzymatic treatment of this aquaculture sludge by batch test for seven days. UNIVERSITI MALAYSIA TERENGGANU 2021-02 Thesis en http://umt-ir.umt.edu.my:8080/handle/123456789/16179 http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/16179/1/Abstract.pdf df92035b31dccaa725c85d3564ffbe34 http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/16179/2/Full%20Thesis%20-%20OOI%20CHEE%20KUAN.pdf 253f876ee4a03454b24cbf94be57beae http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/16179/3/license.txt 8a4605be74aa9ea9d79846c1fba20a33
institution	Universiti Malaysia Terengganu
collection	UMT Repository System
language	English
description	Proteases or proteolytic enzymes, has high potential to be applied in wastewater treatment and improve sludge dewatering. Hence, the ultimate aim of this study is to produce and characterize protease from Aspergillus niger under solid-state fermentation (SSF) by utilizing shrimp shell powder as solid substrate. The produced protease was applied for enzymatic treatment to reduce organic matter and micropollutants in aquaculture sludge based on selected parameters. It was found that the produced protease from SSF was slightly alkaline. The correlation between factors operating parameters (incubation temperature, inoculum size, moisture content) for the production of enzyme are analysed using statistical software, Minitab 16. A 23 full factorial experimental design was employed and the enzyme produced was optimized by the method of desirability function. The optimal conditions for protease production of 3.7 U/mg were 35 °C of incubation temperature, 60% of initial moisture content and 1.0 of inoculum size. It was found that aquaculture sludge collected contained high total suspended solids (TSS) and chemical oxygen demand (COD), which were 8280 mg/L and 5866.67 mg/L respectively. SSF protease and commercial protease was applied for enzymatic treatment of this aquaculture sludge by batch test for seven days.
format	Thesis
author	OOI CHEE KUAN
spellingShingle	OOI CHEE KUAN OPTIMIZATION OF PROTEASE FROM ASPERGILLUS NIGER IN SOLID STATE FERMENTATION FOR ENZYMATIC TREATMENT OF AQUACULTURE SLUDGE
author_facet	OOI CHEE KUAN
author_sort	OOI CHEE KUAN
title	OPTIMIZATION OF PROTEASE FROM ASPERGILLUS NIGER IN SOLID STATE FERMENTATION FOR ENZYMATIC TREATMENT OF AQUACULTURE SLUDGE
title_short	OPTIMIZATION OF PROTEASE FROM ASPERGILLUS NIGER IN SOLID STATE FERMENTATION FOR ENZYMATIC TREATMENT OF AQUACULTURE SLUDGE
title_full	OPTIMIZATION OF PROTEASE FROM ASPERGILLUS NIGER IN SOLID STATE FERMENTATION FOR ENZYMATIC TREATMENT OF AQUACULTURE SLUDGE
title_fullStr	OPTIMIZATION OF PROTEASE FROM ASPERGILLUS NIGER IN SOLID STATE FERMENTATION FOR ENZYMATIC TREATMENT OF AQUACULTURE SLUDGE
title_full_unstemmed	OPTIMIZATION OF PROTEASE FROM ASPERGILLUS NIGER IN SOLID STATE FERMENTATION FOR ENZYMATIC TREATMENT OF AQUACULTURE SLUDGE
title_sort	optimization of protease from aspergillus niger in solid state fermentation for enzymatic treatment of aquaculture sludge
granting_institution	UNIVERSITI MALAYSIA TERENGGANU
url	http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/16179/1/Abstract.pdf http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/16179/2/Full%20Thesis%20-%20OOI%20CHEE%20KUAN.pdf
_version_	1747835892367622144

OPTIMIZATION OF PROTEASE FROM ASPERGILLUS NIGER IN SOLID STATE FERMENTATION FOR ENZYMATIC TREATMENT OF AQUACULTURE SLUDGE

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