Phenotypic And Genotypic Characteristics Of Foodborne Bacteria Associated With Biofilm Formation In Fresh Ulam

Ulam are important food among the Malays because of their nutritional values, and their consumption is increasing. However, the foodborne outbreak which is associated with eating raw ulam may pose health risks to the consumers by increasing crosscontamination, antibiotic-resistant bacteria and...

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Main Author:	Anis Athirah Binti Bahri
Format:	Thesis
Language:	English
Subjects:	Anis Athirah Binti Bahri QH 438.5 .A5 2020
Online Access:	http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/16525/1/QH%20438.5%20.A5%202020%20Abstract.pdf http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/16525/2/QH%20438.5%20.A5%202020%20Full%20text.pdf
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id	my-umt-ir.-16525
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spelling	my-umt-ir.-165252022-04-17T01:20:08Z Phenotypic And Genotypic Characteristics Of Foodborne Bacteria Associated With Biofilm Formation In Fresh Ulam Anis Athirah Binti Bahri Ulam are important food among the Malays because of their nutritional values, and their consumption is increasing. However, the foodborne outbreak which is associated with eating raw ulam may pose health risks to the consumers by increasing crosscontamination, antibiotic-resistant bacteria and biofilm formation. The objectives of this study were to determine the phenotypic characterisation and genotypic identification of E. coli and Salmonella isolated from ulam and their association with biofilm. A total of 32 samples of ulam were randomly collected from wet markets and supermarkets in Kuala Terengganu. The microbiological study was done to enumerate microbial counts and to isolate E. coli and Salmonella using biochemical tests and API20E kit. They were further assessed for phenotypic characteristics such as antibiotic susceptibility testing, colony morphology and biofilm formation. Polymerase chain reaction (PCR) was performed to determine the genotypic identification associated with tetracycline resistance and biofilm formation in E. coli and Salmonella isolates. The microbiological quality of ulam samples were in the range of 6.36-8.83; 4.14-7.48; 0-8.16; 3.94-6.45 log10 CFU/g for aerobic mesophilic bacteria, coliforms, Salmonella and yeast and moulds, respectively. E. coli and Salmonella were detected in 31.3% and 9.4% of ulam samples, respectively. All of the selected isolates were confirmed as E. coli and Salmonella enterica based on the molecular identification using the 16S rRNA and invA gene, respectively. Overall, it was found that the E. coli isolates were highly resistant to cephalothin (78.3%), followed by ampicillin (52.2%), tetracycline (52.2%) and streptomycin (30.4%). Universiti Malaysia Terengganu 2020 Thesis en http://umt-ir.umt.edu.my:8080/handle/123456789/16525 http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/16525/1/QH%20438.5%20.A5%202020%20Abstract.pdf b10e4feca709087fe047b0b5eee09d86 http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/16525/2/QH%20438.5%20.A5%202020%20Full%20text.pdf c242441bb718db101331667d75fcfbb3 http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/16525/3/license.txt 8a4605be74aa9ea9d79846c1fba20a33 Anis Athirah Binti Bahri QH 438.5 .A5 2020
institution	Universiti Malaysia Terengganu
collection	UMT Repository System
language	English
topic	Anis Athirah Binti Bahri QH 438.5 .A5 2020
spellingShingle	Anis Athirah Binti Bahri QH 438.5 .A5 2020 Anis Athirah Binti Bahri Phenotypic And Genotypic Characteristics Of Foodborne Bacteria Associated With Biofilm Formation In Fresh Ulam
description	Ulam are important food among the Malays because of their nutritional values, and their consumption is increasing. However, the foodborne outbreak which is associated with eating raw ulam may pose health risks to the consumers by increasing crosscontamination, antibiotic-resistant bacteria and biofilm formation. The objectives of this study were to determine the phenotypic characterisation and genotypic identification of E. coli and Salmonella isolated from ulam and their association with biofilm. A total of 32 samples of ulam were randomly collected from wet markets and supermarkets in Kuala Terengganu. The microbiological study was done to enumerate microbial counts and to isolate E. coli and Salmonella using biochemical tests and API20E kit. They were further assessed for phenotypic characteristics such as antibiotic susceptibility testing, colony morphology and biofilm formation. Polymerase chain reaction (PCR) was performed to determine the genotypic identification associated with tetracycline resistance and biofilm formation in E. coli and Salmonella isolates. The microbiological quality of ulam samples were in the range of 6.36-8.83; 4.14-7.48; 0-8.16; 3.94-6.45 log10 CFU/g for aerobic mesophilic bacteria, coliforms, Salmonella and yeast and moulds, respectively. E. coli and Salmonella were detected in 31.3% and 9.4% of ulam samples, respectively. All of the selected isolates were confirmed as E. coli and Salmonella enterica based on the molecular identification using the 16S rRNA and invA gene, respectively. Overall, it was found that the E. coli isolates were highly resistant to cephalothin (78.3%), followed by ampicillin (52.2%), tetracycline (52.2%) and streptomycin (30.4%).
format	Thesis
author	Anis Athirah Binti Bahri
author_facet	Anis Athirah Binti Bahri
author_sort	Anis Athirah Binti Bahri
title	Phenotypic And Genotypic Characteristics Of Foodborne Bacteria Associated With Biofilm Formation In Fresh Ulam
title_short	Phenotypic And Genotypic Characteristics Of Foodborne Bacteria Associated With Biofilm Formation In Fresh Ulam
title_full	Phenotypic And Genotypic Characteristics Of Foodborne Bacteria Associated With Biofilm Formation In Fresh Ulam
title_fullStr	Phenotypic And Genotypic Characteristics Of Foodborne Bacteria Associated With Biofilm Formation In Fresh Ulam
title_full_unstemmed	Phenotypic And Genotypic Characteristics Of Foodborne Bacteria Associated With Biofilm Formation In Fresh Ulam
title_sort	phenotypic and genotypic characteristics of foodborne bacteria associated with biofilm formation in fresh ulam
granting_institution	Universiti Malaysia Terengganu
url	http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/16525/1/QH%20438.5%20.A5%202020%20Abstract.pdf http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/16525/2/QH%20438.5%20.A5%202020%20Full%20text.pdf
_version_	1747835778649554944

Phenotypic And Genotypic Characteristics Of Foodborne Bacteria Associated With Biofilm Formation In Fresh Ulam

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