Biomass, Oil and Fatty Acids Profiling Of Mangrove-Isolated Microalgae Under Selected Cultivation Conditions

Microalgae have been recognized for their substantial amount of oil content along with high long-chain marketable polyunsaturated fatty acids (PUFAs) such as omega-3 and -6 fatty acids. However, indigenous microalgae in Malaysia are mostly untapped resources for lipid assessment due to l...

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Main Author:	Nur Syazana Binti Fauzi
Format:	Thesis
Language:	English
Subjects:	QP 752 .E84 N8 2021
Online Access:	http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/16790/1/QP%20752%20.E84%20N8%202021-Abstract.pdf http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/16790/2/QP%20752%20.E84%20N8%202021-Full%20text.pdf
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spelling	my-umt-ir.-167902022-06-21T02:06:10Z Biomass, Oil and Fatty Acids Profiling Of Mangrove-Isolated Microalgae Under Selected Cultivation Conditions Nur Syazana Binti Fauzi Microalgae have been recognized for their substantial amount of oil content along with high long-chain marketable polyunsaturated fatty acids (PUFAs) such as omega-3 and -6 fatty acids. However, indigenous microalgae in Malaysia are mostly untapped resources for lipid assessment due to lack of research conducted, in particular the species from mangrove environment. Therefore, eight new mangrove-isolated microalgae species were successfully described through morphological characterization and molecular identification with 18S rDNA. The identified strains include the species from the genus of Chlorella, Desmodesmus, Chlamydomonas and Scenedesmus. In order to screen these species for potential oil and fatty acid productions, a small-scale cultivation system and an optimized oil extraction protocol were established. This enable cost-saving in term of reduce chemicals usage by 50% and increase efficiency from time-saving. The eight newly characterized species together with two model species (Chlorella vulgaris UMT-MI and Messastrum gracile SE-MC4) were subjected to nitrate-replete and nitrate-deficient cultivation conditions and its growth, biomass, oil content and fatty acid composition were determined. Result revealed that the microalgae could be categorized into high cell growth with high biomass (Group I), moderate cell growth with wider range of biomass (Group 11), and degrading cell growth with low biomass (Group III). Interestingly, five strains (UMT-Ml, SE-MC4, KS-2, UMT-B13 and UMT-B15) were regarded as oleaginous microalgae as they produced more than 20% (in between 23.5• 37.0%) of total oil content under the studied conditions. Furthermore, fatty acid profiling revealed that six strains (UMT-B8, -B13, -B14 and -B15, UMT-MI and KS- 2) were high saturated fatty acid (SFA) producers (or Group H-SFA) with total SFA ranging between 52.4-97.1%. Three strains (SE-MC4, UMT-B10, -B20) were high SFA and monounsaturated fatty acid (MUFA) producers (or Group H-SFA: MUFA) with each constituted more than 30% while one high producer (UMT-B11) for SFA and polyunsaturated fatty acids (or Group H-SFA: PUFA) with each constituted more than 30%. In addition, the influence of different growth phases and culture transition phase on the selected species, M. gracile SE-MC4 were also evaluated. Results showed that the biomass and total oil content increased in parallel to the cultivation day and achieved maximum at late stationary phase (day 8). This species accumulated high amount of Cl 8: 1 (32.4%) at late stationary phase and dropped instantaneously to 13% during the culture transition into Stage II cultivation. This was in contrast to the accumulation of Cl 8:3n3, where its content spiked up from a lowest to a maximum of 23.0%. These demonstrated that the storage Cl8:l pool in the late stationary phase was immediately used as a substrate for the synthesis ofC18:3n3 which could play an important role during the early phase ofmicroalgae growth. The mangrove microalgae can be looked up as a new prospective of lipids and fatty acid sources for various different applications. Understanding the biochemical properties of interest under specific stage of growth is important for future fundamental study to formulate further experiment that could increase the lipid content without compromising its biomass. Universiti Malaysia Terengganu 2021 Thesis en http://umt-ir.umt.edu.my:8080/handle/123456789/16790 http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/16790/3/license.txt 8a4605be74aa9ea9d79846c1fba20a33 http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/16790/1/QP%20752%20.E84%20N8%202021-Abstract.pdf 9274b95d36c9d671377b3687685002c2 http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/16790/2/QP%20752%20.E84%20N8%202021-Full%20text.pdf 928ddb6e1b032f9107579dac4cdbf746 QP 752 .E84 N8 2021
institution	Universiti Malaysia Terengganu
collection	UMT Repository System
language	English
topic	QP 752 .E84 N8 2021
spellingShingle	QP 752 .E84 N8 2021 Nur Syazana Binti Fauzi Biomass, Oil and Fatty Acids Profiling Of Mangrove-Isolated Microalgae Under Selected Cultivation Conditions
description	Microalgae have been recognized for their substantial amount of oil content along with high long-chain marketable polyunsaturated fatty acids (PUFAs) such as omega-3 and -6 fatty acids. However, indigenous microalgae in Malaysia are mostly untapped resources for lipid assessment due to lack of research conducted, in particular the species from mangrove environment. Therefore, eight new mangrove-isolated microalgae species were successfully described through morphological characterization and molecular identification with 18S rDNA. The identified strains include the species from the genus of Chlorella, Desmodesmus, Chlamydomonas and Scenedesmus. In order to screen these species for potential oil and fatty acid productions, a small-scale cultivation system and an optimized oil extraction protocol were established. This enable cost-saving in term of reduce chemicals usage by 50% and increase efficiency from time-saving. The eight newly characterized species together with two model species (Chlorella vulgaris UMT-MI and Messastrum gracile SE-MC4) were subjected to nitrate-replete and nitrate-deficient cultivation conditions and its growth, biomass, oil content and fatty acid composition were determined. Result revealed that the microalgae could be categorized into high cell growth with high biomass (Group I), moderate cell growth with wider range of biomass (Group 11), and degrading cell growth with low biomass (Group III). Interestingly, five strains (UMT-Ml, SE-MC4, KS-2, UMT-B13 and UMT-B15) were regarded as oleaginous microalgae as they produced more than 20% (in between 23.5• 37.0%) of total oil content under the studied conditions. Furthermore, fatty acid profiling revealed that six strains (UMT-B8, -B13, -B14 and -B15, UMT-MI and KS- 2) were high saturated fatty acid (SFA) producers (or Group H-SFA) with total SFA ranging between 52.4-97.1%. Three strains (SE-MC4, UMT-B10, -B20) were high SFA and monounsaturated fatty acid (MUFA) producers (or Group H-SFA: MUFA) with each constituted more than 30% while one high producer (UMT-B11) for SFA and polyunsaturated fatty acids (or Group H-SFA: PUFA) with each constituted more than 30%. In addition, the influence of different growth phases and culture transition phase on the selected species, M. gracile SE-MC4 were also evaluated. Results showed that the biomass and total oil content increased in parallel to the cultivation day and achieved maximum at late stationary phase (day 8). This species accumulated high amount of Cl 8: 1 (32.4%) at late stationary phase and dropped instantaneously to 13% during the culture transition into Stage II cultivation. This was in contrast to the accumulation of Cl 8:3n3, where its content spiked up from a lowest to a maximum of 23.0%. These demonstrated that the storage Cl8:l pool in the late stationary phase was immediately used as a substrate for the synthesis ofC18:3n3 which could play an important role during the early phase ofmicroalgae growth. The mangrove microalgae can be looked up as a new prospective of lipids and fatty acid sources for various different applications. Understanding the biochemical properties of interest under specific stage of growth is important for future fundamental study to formulate further experiment that could increase the lipid content without compromising its biomass.
format	Thesis
author	Nur Syazana Binti Fauzi
author_facet	Nur Syazana Binti Fauzi
author_sort	Nur Syazana Binti Fauzi
title	Biomass, Oil and Fatty Acids Profiling Of Mangrove-Isolated Microalgae Under Selected Cultivation Conditions
title_short	Biomass, Oil and Fatty Acids Profiling Of Mangrove-Isolated Microalgae Under Selected Cultivation Conditions
title_full	Biomass, Oil and Fatty Acids Profiling Of Mangrove-Isolated Microalgae Under Selected Cultivation Conditions
title_fullStr	Biomass, Oil and Fatty Acids Profiling Of Mangrove-Isolated Microalgae Under Selected Cultivation Conditions
title_full_unstemmed	Biomass, Oil and Fatty Acids Profiling Of Mangrove-Isolated Microalgae Under Selected Cultivation Conditions
title_sort	biomass, oil and fatty acids profiling of mangrove-isolated microalgae under selected cultivation conditions
granting_institution	Universiti Malaysia Terengganu
url	http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/16790/1/QP%20752%20.E84%20N8%202021-Abstract.pdf http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/16790/2/QP%20752%20.E84%20N8%202021-Full%20text.pdf
_version_	1747835780101832704

Biomass, Oil and Fatty Acids Profiling Of Mangrove-Isolated Microalgae Under Selected Cultivation Conditions

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