Development of agrobacterium-mediated transformation method for marine microalgae (Chlorella sp. and Nannochloropsis sp.)

An important step in the development of an efficient Agrobacterium-medialed transformation system is the establishment of optimal conditions for efficient TDNA transfer. Parameters known to influence the efficacy of Agrobacterium tumefaciens T-DNA transfer such as pre-culture of algal cells, inoc...

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Main Author:	Willy, Yee
Format:	Thesis
Language:	English
Subjects:	QR 82 .R45 W5 2011 Tesis FST 2011 Agrobacterium
Online Access:	http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/1967/1/QR%2082%20.R45%20W5%202011%20Abstract.pdf http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/1967/2/QR%2082%20.R45%20W5%202011%20FullText.pdf
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id	my-umt-ir.-1967
record_format	uketd_dc
spelling	my-umt-ir.-19672012-10-08T03:43:16Z Development of agrobacterium-mediated transformation method for marine microalgae (Chlorella sp. and Nannochloropsis sp.) Willy, Yee An important step in the development of an efficient Agrobacterium-medialed transformation system is the establishment of optimal conditions for efficient TDNA transfer. Parameters known to influence the efficacy of Agrobacterium tumefaciens T-DNA transfer such as pre-culture of algal cells, inoculum density, cocultivation temperature, co-cultivation duration, pH of co-cultivation medium and the concentration of acetosyringone were evaluated by monitoring transient GUS expression two days post-infection. Terengganu : Universiti Malaysia Terengganu 2011-02 Thesis en http://hdl.handle.net/123456789/1967 http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/1967/1/QR%2082%20.R45%20W5%202011%20Abstract.pdf 90ab146b31083617f553e36b825257f1 http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/1967/2/QR%2082%20.R45%20W5%202011%20FullText.pdf a977689ce2de306baadc22bd02f3f074 http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/1967/3/license.txt 8a4605be74aa9ea9d79846c1fba20a33 QR 82 .R45 W5 2011 Willy, Yee Tesis FST 2011 Agrobacterium
institution	Universiti Malaysia Terengganu
collection	UMT Repository System
language	English
topic	QR 82 .R45 W5 2011 QR 82 .R45 W5 2011 Tesis FST 2011 Agrobacterium
spellingShingle	QR 82 .R45 W5 2011 QR 82 .R45 W5 2011 Tesis FST 2011 Agrobacterium Willy, Yee Development of agrobacterium-mediated transformation method for marine microalgae (Chlorella sp. and Nannochloropsis sp.)
description	An important step in the development of an efficient Agrobacterium-medialed transformation system is the establishment of optimal conditions for efficient TDNA transfer. Parameters known to influence the efficacy of Agrobacterium tumefaciens T-DNA transfer such as pre-culture of algal cells, inoculum density, cocultivation temperature, co-cultivation duration, pH of co-cultivation medium and the concentration of acetosyringone were evaluated by monitoring transient GUS expression two days post-infection.
format	Thesis
author	Willy, Yee
author_facet	Willy, Yee
author_sort	Willy, Yee
title	Development of agrobacterium-mediated transformation method for marine microalgae (Chlorella sp. and Nannochloropsis sp.)
title_short	Development of agrobacterium-mediated transformation method for marine microalgae (Chlorella sp. and Nannochloropsis sp.)
title_full	Development of agrobacterium-mediated transformation method for marine microalgae (Chlorella sp. and Nannochloropsis sp.)
title_fullStr	Development of agrobacterium-mediated transformation method for marine microalgae (Chlorella sp. and Nannochloropsis sp.)
title_full_unstemmed	Development of agrobacterium-mediated transformation method for marine microalgae (Chlorella sp. and Nannochloropsis sp.)
title_sort	development of agrobacterium-mediated transformation method for marine microalgae (chlorella sp. and nannochloropsis sp.)
granting_institution	Terengganu : Universiti Malaysia Terengganu
url	http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/1967/1/QR%2082%20.R45%20W5%202011%20Abstract.pdf http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/1967/2/QR%2082%20.R45%20W5%202011%20FullText.pdf
_version_	1747835842808774656

Development of agrobacterium-mediated transformation method for marine microalgae (Chlorella sp. and Nannochloropsis sp.)

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