Molecular characterization of Citrobacter freundii, Edwardsiella tarda and Aeromanas hydrophila isolated from red tilapia (Oreochromis sp.) fingerlings cultured at Terengganu and Selangor

Red tilapia has been an important food fish that ranked on top of all freshwater aquaculture species in Malaysia. Nevertheless, productivity and sustainability of the red tilapia can be affected once diseases struck. In present study, phenotypic and genotypic characterization of fish disease causati...

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Main Author:	Wee, Wendy
Format:	Thesis
Language:	English
Subjects:	QH 506 .W4 2008 Molecular biology
Online Access:	http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/592/1/QH506%20.W4%202008%20Abstract.pdf http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/592/2/QH506%20.W4%202008.pdf
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spelling	my-umt-ir.-5922016-03-10T05:46:25Z Molecular characterization of Citrobacter freundii, Edwardsiella tarda and Aeromanas hydrophila isolated from red tilapia (Oreochromis sp.) fingerlings cultured at Terengganu and Selangor Wee, Wendy Red tilapia has been an important food fish that ranked on top of all freshwater aquaculture species in Malaysia. Nevertheless, productivity and sustainability of the red tilapia can be affected once diseases struck. In present study, phenotypic and genotypic characterization of fish disease causative agents has been focus on Citrobacter freundii, Edwardsiella tarda and Aeromonas hydrophila. A total number of 45 isolates were obtained from red tilapia fingerlings farmed at Terengganu and Selangor. Presumptive isolation of C. freundii, E. tarda and A. hydrophila has been made from moderate dark pink colony on Cefsulodin-Irgasan-Novobiocin (CIN) agar, tiny clear transparent colony with black dot on Xylose-Lysine-Deoxycholate (XLD) agar, and moderate yellow colony on Glutamate Starch Phenol red (GSP) agar, respectively. Meanwhile, bacterial identification was accomplished by 52 biochemical and physiological tests by conventional method and BBL Crystal™ Enteric/ Nonfermenter Identification Systems (E/NF ID). The production of hemolysins which lysed on blood agar has been regarded as strong evidence of pathogenic potential in A. hydrophila isolated from Terengganu farm despite that majority of the isolates were lack of hemolytic activity Fakulti Sains dan Teknologi 2008-06 Thesis en http://hdl.handle.net/123456789/592 http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/592/1/QH506%20.W4%202008%20Abstract.pdf 9a62187d4ce3a9fa1cdf33e8569ca737 http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/592/2/QH506%20.W4%202008.pdf 659dd6a72e05050728f100369717e77c http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/592/3/license.txt 8a4605be74aa9ea9d79846c1fba20a33 http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/592/4/QH506%20.W4%202008%20Abstract.pdf.txt da91484b348d5eabf7c00489baa7fdec http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/592/5/QH506%20.W4%202008.pdf.txt cd948cf198a6b6ec0c459ca6e5f0a784 QH 506 .W4 2008 Wee, Wendy Molecular biology Molecular characterization of Citrobacter freundii, Edwardsiella tarda and Aeromanas hydrophila isolated from red tilapia (Oreochromis sp.) fingerlings cultured at Terengganu and Selangor
institution	Universiti Malaysia Terengganu
collection	UMT Repository System
language	English
topic	QH 506 .W4 2008 QH 506 .W4 2008 Molecular biology QH 506 .W4 2008
spellingShingle	QH 506 .W4 2008 QH 506 .W4 2008 Molecular biology QH 506 .W4 2008 Wee, Wendy Molecular characterization of Citrobacter freundii, Edwardsiella tarda and Aeromanas hydrophila isolated from red tilapia (Oreochromis sp.) fingerlings cultured at Terengganu and Selangor
description	Red tilapia has been an important food fish that ranked on top of all freshwater aquaculture species in Malaysia. Nevertheless, productivity and sustainability of the red tilapia can be affected once diseases struck. In present study, phenotypic and genotypic characterization of fish disease causative agents has been focus on Citrobacter freundii, Edwardsiella tarda and Aeromonas hydrophila. A total number of 45 isolates were obtained from red tilapia fingerlings farmed at Terengganu and Selangor. Presumptive isolation of C. freundii, E. tarda and A. hydrophila has been made from moderate dark pink colony on Cefsulodin-Irgasan-Novobiocin (CIN) agar, tiny clear transparent colony with black dot on Xylose-Lysine-Deoxycholate (XLD) agar, and moderate yellow colony on Glutamate Starch Phenol red (GSP) agar, respectively. Meanwhile, bacterial identification was accomplished by 52 biochemical and physiological tests by conventional method and BBL Crystal™ Enteric/ Nonfermenter Identification Systems (E/NF ID). The production of hemolysins which lysed on blood agar has been regarded as strong evidence of pathogenic potential in A. hydrophila isolated from Terengganu farm despite that majority of the isolates were lack of hemolytic activity
format	Thesis
author	Wee, Wendy
author_facet	Wee, Wendy
author_sort	Wee, Wendy
title	Molecular characterization of Citrobacter freundii, Edwardsiella tarda and Aeromanas hydrophila isolated from red tilapia (Oreochromis sp.) fingerlings cultured at Terengganu and Selangor
title_short	Molecular characterization of Citrobacter freundii, Edwardsiella tarda and Aeromanas hydrophila isolated from red tilapia (Oreochromis sp.) fingerlings cultured at Terengganu and Selangor
title_full	Molecular characterization of Citrobacter freundii, Edwardsiella tarda and Aeromanas hydrophila isolated from red tilapia (Oreochromis sp.) fingerlings cultured at Terengganu and Selangor
title_fullStr	Molecular characterization of Citrobacter freundii, Edwardsiella tarda and Aeromanas hydrophila isolated from red tilapia (Oreochromis sp.) fingerlings cultured at Terengganu and Selangor
title_full_unstemmed	Molecular characterization of Citrobacter freundii, Edwardsiella tarda and Aeromanas hydrophila isolated from red tilapia (Oreochromis sp.) fingerlings cultured at Terengganu and Selangor
title_sort	molecular characterization of citrobacter freundii, edwardsiella tarda and aeromanas hydrophila isolated from red tilapia (oreochromis sp.) fingerlings cultured at terengganu and selangor
granting_institution	Fakulti Sains dan Teknologi
url	http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/592/1/QH506%20.W4%202008%20Abstract.pdf http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/592/2/QH506%20.W4%202008.pdf
_version_	1747835800001708032

Molecular characterization of Citrobacter freundii, Edwardsiella tarda and Aeromanas hydrophila isolated from red tilapia (Oreochromis sp.) fingerlings cultured at Terengganu and Selangor

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