Study on genetic variability of oyster (Crassostrea iredalei, Faustino)in Peninsular Malaysia using RAPD-PCR Technique

The random amplified polymorphic DNA (RAPD) technique was used to examine the genetic variability and relationship among individuals within and between populations of oysters (Crassostrea iredalei) from Terengganu, Kelantan, Perak and Kedah. The result of optimization of polymerase chain reaction (...

Full description

Saved in:

Bibliographic Details
Main Author:	Wan Bayani, Wan Omar
Format:	Thesis
Language:	English
Subjects:	SH 371 .W3 2003 Wan Bayani Wan Omar Oyster culture
Online Access:	http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/599/1/SH371%20.W3%202003%20Abstract.pdf http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/599/2/SH371%20.W3%202003.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-umt-ir.-599
record_format	uketd_dc
spelling	my-umt-ir.-5992016-03-10T07:42:43Z Study on genetic variability of oyster (Crassostrea iredalei, Faustino)in Peninsular Malaysia using RAPD-PCR Technique Wan Bayani, Wan Omar The random amplified polymorphic DNA (RAPD) technique was used to examine the genetic variability and relationship among individuals within and between populations of oysters (Crassostrea iredalei) from Terengganu, Kelantan, Perak and Kedah. The result of optimization of polymerase chain reaction (PCR) machine (Hybaid PCR Express, UK) showed that the best concentration of genomic DNA, magnesium chloride, dNTP- mixture, Fermentas Taq DNA Polymerase and primer for C. iredalei RAPD study were 50 ng, 3.0 mM, 0.4 mM, 2 U/\|il and 0.4 \|iM (10 picomoles) respectively. The machine produced clear RAPD banding patterns with an optimal annealing temperature of 36°C for 45 cycles. The genomic DNA was extracted from the oysters tissues by using phenol chloroform method. Twenty oligonucleotide primers (Kit A, Operon Technologies, Inc. Alameda, California) were screened and four primers were selected to amplify DNA from 80 samples of C. iredalei from the four populations. A total of 159 RAPD fragments (RAPDs) with 122 polymorphic fragments (76.73%) with the size ranging from 250 to 2000 base pairs (bp) were scored from the population. Fakulti Agroteknologi dan Sains Makanan 2003-09 Thesis en http://hdl.handle.net/123456789/599 http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/599/1/SH371%20.W3%202003%20Abstract.pdf 58fcf406a72f8e029154e9be93632d5e http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/599/2/SH371%20.W3%202003.pdf f16991a01dc5d4840d0ab058a2b7b9fe http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/599/3/license.txt 8a4605be74aa9ea9d79846c1fba20a33 http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/599/4/SH371%20.W3%202003%20Abstract.pdf.txt 2941d31a083ed0018de10839d793f8ff http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/599/5/SH371%20.W3%202003.pdf.txt 38cbea4a98475e643ea97a1c11e9fb39 SH 371 .W3 2003 Wan Bayani Wan Omar Study on genetic variability of oyster (Crassostrea iredalei, Faustino)in Peninsular Malaysia using RAPD-PCR Technique Oyster culture
institution	Universiti Malaysia Terengganu
collection	UMT Repository System
language	English
topic	SH 371 .W3 2003 Wan Bayani Wan Omar SH 371 .W3 2003 Oyster culture
spellingShingle	SH 371 .W3 2003 Wan Bayani Wan Omar SH 371 .W3 2003 Oyster culture Wan Bayani, Wan Omar Study on genetic variability of oyster (Crassostrea iredalei, Faustino)in Peninsular Malaysia using RAPD-PCR Technique
description	The random amplified polymorphic DNA (RAPD) technique was used to examine the genetic variability and relationship among individuals within and between populations of oysters (Crassostrea iredalei) from Terengganu, Kelantan, Perak and Kedah. The result of optimization of polymerase chain reaction (PCR) machine (Hybaid PCR Express, UK) showed that the best concentration of genomic DNA, magnesium chloride, dNTP- mixture, Fermentas Taq DNA Polymerase and primer for C. iredalei RAPD study were 50 ng, 3.0 mM, 0.4 mM, 2 U/\|il and 0.4 \|iM (10 picomoles) respectively. The machine produced clear RAPD banding patterns with an optimal annealing temperature of 36°C for 45 cycles. The genomic DNA was extracted from the oysters tissues by using phenol chloroform method. Twenty oligonucleotide primers (Kit A, Operon Technologies, Inc. Alameda, California) were screened and four primers were selected to amplify DNA from 80 samples of C. iredalei from the four populations. A total of 159 RAPD fragments (RAPDs) with 122 polymorphic fragments (76.73%) with the size ranging from 250 to 2000 base pairs (bp) were scored from the population.
format	Thesis
author	Wan Bayani, Wan Omar
author_facet	Wan Bayani, Wan Omar
author_sort	Wan Bayani, Wan Omar
title	Study on genetic variability of oyster (Crassostrea iredalei, Faustino)in Peninsular Malaysia using RAPD-PCR Technique
title_short	Study on genetic variability of oyster (Crassostrea iredalei, Faustino)in Peninsular Malaysia using RAPD-PCR Technique
title_full	Study on genetic variability of oyster (Crassostrea iredalei, Faustino)in Peninsular Malaysia using RAPD-PCR Technique
title_fullStr	Study on genetic variability of oyster (Crassostrea iredalei, Faustino)in Peninsular Malaysia using RAPD-PCR Technique
title_full_unstemmed	Study on genetic variability of oyster (Crassostrea iredalei, Faustino)in Peninsular Malaysia using RAPD-PCR Technique
title_sort	study on genetic variability of oyster (crassostrea iredalei, faustino)in peninsular malaysia using rapd-pcr technique
granting_institution	Fakulti Agroteknologi dan Sains Makanan
url	http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/599/1/SH371%20.W3%202003%20Abstract.pdf http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/599/2/SH371%20.W3%202003.pdf
_version_	1747835800489295872

Study on genetic variability of oyster (Crassostrea iredalei, Faustino)in Peninsular Malaysia using RAPD-PCR Technique

Similar Items