Study on genetic variability of oyster (Crassostrea iredalei, Faustino)in Peninsular Malaysia using RAPD-PCR Technique
The random amplified polymorphic DNA (RAPD) technique was used to examine the genetic variability and relationship among individuals within and between populations of oysters (Crassostrea iredalei) from Terengganu, Kelantan, Perak and Kedah. The result of optimization of polymerase chain reaction (...
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my-umt-ir.-5992016-03-10T07:42:43Z Study on genetic variability of oyster (Crassostrea iredalei, Faustino)in Peninsular Malaysia using RAPD-PCR Technique Wan Bayani, Wan Omar The random amplified polymorphic DNA (RAPD) technique was used to examine the genetic variability and relationship among individuals within and between populations of oysters (Crassostrea iredalei) from Terengganu, Kelantan, Perak and Kedah. The result of optimization of polymerase chain reaction (PCR) machine (Hybaid PCR Express, UK) showed that the best concentration of genomic DNA, magnesium chloride, dNTP- mixture, Fermentas Taq DNA Polymerase and primer for C. iredalei RAPD study were 50 ng, 3.0 mM, 0.4 mM, 2 U/|il and 0.4 |iM (10 picomoles) respectively. The machine produced clear RAPD banding patterns with an optimal annealing temperature of 36°C for 45 cycles. The genomic DNA was extracted from the oysters tissues by using phenol chloroform method. Twenty oligonucleotide primers (Kit A, Operon Technologies, Inc. Alameda, California) were screened and four primers were selected to amplify DNA from 80 samples of C. iredalei from the four populations. A total of 159 RAPD fragments (RAPDs) with 122 polymorphic fragments (76.73%) with the size ranging from 250 to 2000 base pairs (bp) were scored from the population. Fakulti Agroteknologi dan Sains Makanan 2003-09 Thesis en http://hdl.handle.net/123456789/599 http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/599/1/SH371%20.W3%202003%20Abstract.pdf 58fcf406a72f8e029154e9be93632d5e http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/599/2/SH371%20.W3%202003.pdf f16991a01dc5d4840d0ab058a2b7b9fe http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/599/3/license.txt 8a4605be74aa9ea9d79846c1fba20a33 http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/599/4/SH371%20.W3%202003%20Abstract.pdf.txt 2941d31a083ed0018de10839d793f8ff http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/599/5/SH371%20.W3%202003.pdf.txt 38cbea4a98475e643ea97a1c11e9fb39 SH 371 .W3 2003 Wan Bayani Wan Omar Study on genetic variability of oyster (Crassostrea iredalei, Faustino)in Peninsular Malaysia using RAPD-PCR Technique Oyster culture |
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Universiti Malaysia Terengganu |
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| language |
English |
| topic |
SH 371 .W3 2003 Wan Bayani Wan Omar SH 371 .W3 2003 Oyster culture |
| spellingShingle |
SH 371 .W3 2003 Wan Bayani Wan Omar SH 371 .W3 2003 Oyster culture Wan Bayani, Wan Omar Study on genetic variability of oyster (Crassostrea iredalei, Faustino)in Peninsular Malaysia using RAPD-PCR Technique |
| description |
The random amplified polymorphic DNA (RAPD) technique was used to examine the genetic variability and relationship among individuals within and between populations of
oysters (Crassostrea iredalei) from Terengganu, Kelantan, Perak and Kedah. The result of optimization of polymerase chain reaction (PCR) machine (Hybaid PCR Express, UK) showed that the best concentration of genomic DNA, magnesium chloride, dNTP- mixture, Fermentas Taq DNA Polymerase and primer for C. iredalei RAPD study were 50 ng, 3.0 mM, 0.4 mM, 2 U/|il and 0.4 |iM (10 picomoles) respectively. The machine
produced clear RAPD banding patterns with an optimal annealing temperature of 36°C for 45 cycles. The genomic DNA was extracted from the oysters tissues by using phenol chloroform method. Twenty oligonucleotide primers (Kit A, Operon Technologies, Inc.
Alameda, California) were screened and four primers were selected to amplify DNA from
80 samples of C. iredalei from the four populations. A total of 159 RAPD fragments
(RAPDs) with 122 polymorphic fragments (76.73%) with the size ranging from 250 to
2000 base pairs (bp) were scored from the population. |
| format |
Thesis |
| author |
Wan Bayani, Wan Omar |
| author_facet |
Wan Bayani, Wan Omar |
| author_sort |
Wan Bayani, Wan Omar |
| title |
Study on genetic variability of oyster (Crassostrea iredalei, Faustino)in Peninsular Malaysia using RAPD-PCR Technique |
| title_short |
Study on genetic variability of oyster (Crassostrea iredalei, Faustino)in Peninsular Malaysia using RAPD-PCR Technique |
| title_full |
Study on genetic variability of oyster (Crassostrea iredalei, Faustino)in Peninsular Malaysia using RAPD-PCR Technique |
| title_fullStr |
Study on genetic variability of oyster (Crassostrea iredalei, Faustino)in Peninsular Malaysia using RAPD-PCR Technique |
| title_full_unstemmed |
Study on genetic variability of oyster (Crassostrea iredalei, Faustino)in Peninsular Malaysia using RAPD-PCR Technique |
| title_sort |
study on genetic variability of oyster (crassostrea iredalei, faustino)in peninsular malaysia using rapd-pcr technique |
| granting_institution |
Fakulti Agroteknologi dan Sains Makanan |
| url |
http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/599/1/SH371%20.W3%202003%20Abstract.pdf http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/599/2/SH371%20.W3%202003.pdf |
| _version_ |
1747835800489295872 |