Studies on the population genetics of green (Chelonia mydas) and hawksbill (Eretmochelys imbricata) turtles in Malaysia using DNA microsatellites
Genetic diversity of six populations of green turtle (Chelonia mydas Linnaeus, 1958) from Terengganu, Pahang, Perak, Sarawak, Sabah Turtle Islands Park and Pulau Sipadan and four populations of hawksbill turtle (Eretmochelys imbricata Linnaeus, 1766) from Melaka, Johor, Terengganu and Sabah were ana...
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my-umt-ir.-6332016-03-10T05:51:07Z Studies on the population genetics of green (Chelonia mydas) and hawksbill (Eretmochelys imbricata) turtles in Malaysia using DNA microsatellites Juanita, Joseph Genetic diversity of six populations of green turtle (Chelonia mydas Linnaeus, 1958) from Terengganu, Pahang, Perak, Sarawak, Sabah Turtle Islands Park and Pulau Sipadan and four populations of hawksbill turtle (Eretmochelys imbricata Linnaeus, 1766) from Melaka, Johor, Terengganu and Sabah were analysed using 14 microsatellite markers. The amplified DNA product was electrophoresed using Nusieve Agarose gel 3:1 and the banding patterns were visualized by using UV light. High levels of polymorphism were observed over all populations of green turtle, with up to 12 alleles per locus and expected heterozygosities ranging from 0.5 to 0.7. In hawksbill turtle populations, these loci were also polymorphic, with up to 8 alleles per locus and expected heterozygosities from 0.2 to 0.6. The difference in the level of genetic variability in the two species of marine turtles needs explanation and may have profound implications for the management of these endangered species. Fakulti Sains dan Teknologi 2000-11 Thesis en http://hdl.handle.net/123456789/633 http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/633/1/SH399%20.T9%20J8%202000%20Abstract.pdf e53d94445726bc4750e939459b084b7c http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/633/2/SH399%20.T9%20J8%202000.pdf 3adfb7888f84445d15699c97e278ddad http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/633/3/license.txt 8a4605be74aa9ea9d79846c1fba20a33 http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/633/4/SH399%20.T9%20J8%202000%20Abstract.pdf.txt e050ce1989f2c7168c909725d42d2b7a http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/633/5/SH399%20.T9%20J8%202000.pdf.txt 1c82bd0b8a9c6cb19e6a4689dd5da008 SH 399 .T9 J8 2000 Juanita Joseph Studies on the population genetics of green (Chelonia mydas) and hawksbill (Eretmochelys imbricata) turtles in Malaysia using DNA microsatellites Genetics Genetics -- Research Genetics, Experimental Turtle culture Turtles |
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SH 399 .T9 J8 2000 Juanita Joseph Studies on the population genetics of green (Chelonia mydas) and hawksbill (Eretmochelys imbricata) turtles in Malaysia using DNA microsatellites Genetics Genetics -- Research SH 399 .T9 J8 2000 Turtle culture Turtles |
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SH 399 .T9 J8 2000 Juanita Joseph Studies on the population genetics of green (Chelonia mydas) and hawksbill (Eretmochelys imbricata) turtles in Malaysia using DNA microsatellites Genetics Genetics -- Research SH 399 .T9 J8 2000 Turtle culture Turtles Juanita, Joseph Studies on the population genetics of green (Chelonia mydas) and hawksbill (Eretmochelys imbricata) turtles in Malaysia using DNA microsatellites |
description |
Genetic diversity of six populations of green turtle (Chelonia mydas Linnaeus, 1958) from Terengganu, Pahang, Perak, Sarawak, Sabah Turtle Islands Park and Pulau Sipadan and four populations of hawksbill turtle (Eretmochelys imbricata Linnaeus, 1766) from Melaka, Johor, Terengganu and Sabah were analysed using 14 microsatellite markers. The amplified DNA product was electrophoresed using Nusieve Agarose gel 3:1 and the banding patterns were visualized by using UV light. High levels of polymorphism were observed over all populations of green turtle, with up to 12 alleles per locus and expected heterozygosities ranging from 0.5 to 0.7. In hawksbill turtle populations, these loci were also polymorphic, with up to 8 alleles per locus and expected heterozygosities from 0.2 to 0.6. The difference in the level of genetic variability in the two species of marine turtles needs explanation and may have profound implications for the management of these endangered species. |
format |
Thesis |
author |
Juanita, Joseph |
author_facet |
Juanita, Joseph |
author_sort |
Juanita, Joseph |
title |
Studies on the population genetics of green (Chelonia mydas) and hawksbill (Eretmochelys imbricata) turtles in Malaysia using DNA microsatellites |
title_short |
Studies on the population genetics of green (Chelonia mydas) and hawksbill (Eretmochelys imbricata) turtles in Malaysia using DNA microsatellites |
title_full |
Studies on the population genetics of green (Chelonia mydas) and hawksbill (Eretmochelys imbricata) turtles in Malaysia using DNA microsatellites |
title_fullStr |
Studies on the population genetics of green (Chelonia mydas) and hawksbill (Eretmochelys imbricata) turtles in Malaysia using DNA microsatellites |
title_full_unstemmed |
Studies on the population genetics of green (Chelonia mydas) and hawksbill (Eretmochelys imbricata) turtles in Malaysia using DNA microsatellites |
title_sort |
studies on the population genetics of green (chelonia mydas) and hawksbill (eretmochelys imbricata) turtles in malaysia using dna microsatellites |
granting_institution |
Fakulti Sains dan Teknologi |
url |
http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/633/1/SH399%20.T9%20J8%202000%20Abstract.pdf http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/633/2/SH399%20.T9%20J8%202000.pdf |
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