Studies on genetic variability of groupers (Epinephelus spp.) from Indo-Malaysian waters using PCR-RAPD analysis

The serranid subfamily {Epinephelus genus), popularly known as grouper, is one of the most economically important fishes in Southeast Asian countries. In this study, RAPD in association with PCR was employed to determine the genetic variability and to establish the RAPD fingerprinting of groupers...

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Main Author:	Parenrengi, Andi
Format:	Thesis
Language:	English
Subjects:	QL 638 .S48 P3 2001 Studies on genetic variability of groupers (Epinephelus spp.) from Indo-Malaysian waters using PCR-RAPD analysis Groupers > Malaysia Serranidae
Online Access:	http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/648/3/QL638%20.S48%20P3%202001%20Abstract.pdf http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/648/4/QL638%20.S48%20P3%202001.pdf
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id	my-umt-ir.-648
record_format	uketd_dc
spelling	my-umt-ir.-6482016-03-10T07:44:16Z Studies on genetic variability of groupers (Epinephelus spp.) from Indo-Malaysian waters using PCR-RAPD analysis Parenrengi, Andi The serranid subfamily {Epinephelus genus), popularly known as grouper, is one of the most economically important fishes in Southeast Asian countries. In this study, RAPD in association with PCR was employed to determine the genetic variability and to establish the RAPD fingerprinting of groupers {Epinephelus spp.). A total of 100 individuals of E. tauvina from six populations taken from Indo-Malaysian waters and 36 individuals of E. merra and E. areolatus taken from Makassar Strait, South Sulawesi were studied. Three tissue preservatives and four DNA extraction techniques were employed to find the best preservative and DNA extraction technique for grouper. A series of optimization experiments of PCR-RAPD protocol were conducted. Out of the 34 screened random primers, ten primers were selected for further analysis. Fakulti Sains dan Teknologi 2001-05 Thesis en http://hdl.handle.net/123456789/648 http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/648/3/QL638%20.S48%20P3%202001%20Abstract.pdf a272ac7d8e380ad2199357a2c016f2de http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/648/4/QL638%20.S48%20P3%202001.pdf 0919d9695526186a08daa5f23d9a981a http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/648/2/license.txt 8a4605be74aa9ea9d79846c1fba20a33 http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/648/5/QL638%20.S48%20P3%202001%20Abstract.pdf.txt 0ff8137e35832b4c6bdbd98228a4f86c http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/648/6/QL638%20.S48%20P3%202001.pdf.txt 04674a3e7234bf1dd66ed5741ff584d9 QL 638 .S48 P3 2001 Parenrengi, Andi Studies on genetic variability of groupers (Epinephelus spp.) from Indo-Malaysian waters using PCR-RAPD analysis Groupers -- Malaysia Serranidae
institution	Universiti Malaysia Terengganu
collection	UMT Repository System
language	English
topic	QL 638 .S48 P3 2001 QL 638 .S48 P3 2001 Studies on genetic variability of groupers (Epinephelus spp.) from Indo-Malaysian waters using PCR-RAPD analysis Groupers -- Malaysia Serranidae
spellingShingle	QL 638 .S48 P3 2001 QL 638 .S48 P3 2001 Studies on genetic variability of groupers (Epinephelus spp.) from Indo-Malaysian waters using PCR-RAPD analysis Groupers -- Malaysia Serranidae Parenrengi, Andi Studies on genetic variability of groupers (Epinephelus spp.) from Indo-Malaysian waters using PCR-RAPD analysis
description	The serranid subfamily {Epinephelus genus), popularly known as grouper, is one of the most economically important fishes in Southeast Asian countries. In this study, RAPD in association with PCR was employed to determine the genetic variability and to establish the RAPD fingerprinting of groupers {Epinephelus spp.). A total of 100 individuals of E. tauvina from six populations taken from Indo-Malaysian waters and 36 individuals of E. merra and E. areolatus taken from Makassar Strait, South Sulawesi were studied. Three tissue preservatives and four DNA extraction techniques were employed to find the best preservative and DNA extraction technique for grouper. A series of optimization experiments of PCR-RAPD protocol were conducted. Out of the 34 screened random primers, ten primers were selected for further analysis.
format	Thesis
author	Parenrengi, Andi
author_facet	Parenrengi, Andi
author_sort	Parenrengi, Andi
title	Studies on genetic variability of groupers (Epinephelus spp.) from Indo-Malaysian waters using PCR-RAPD analysis
title_short	Studies on genetic variability of groupers (Epinephelus spp.) from Indo-Malaysian waters using PCR-RAPD analysis
title_full	Studies on genetic variability of groupers (Epinephelus spp.) from Indo-Malaysian waters using PCR-RAPD analysis
title_fullStr	Studies on genetic variability of groupers (Epinephelus spp.) from Indo-Malaysian waters using PCR-RAPD analysis
title_full_unstemmed	Studies on genetic variability of groupers (Epinephelus spp.) from Indo-Malaysian waters using PCR-RAPD analysis
title_sort	studies on genetic variability of groupers (epinephelus spp.) from indo-malaysian waters using pcr-rapd analysis
granting_institution	Fakulti Sains dan Teknologi
url	http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/648/3/QL638%20.S48%20P3%202001%20Abstract.pdf http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/648/4/QL638%20.S48%20P3%202001.pdf
_version_	1747835802692354048

Studies on genetic variability of groupers (Epinephelus spp.) from Indo-Malaysian waters using PCR-RAPD analysis

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