Studies on genetic variability of groupers (Epinephelus spp.) from Indo-Malaysian waters using PCR-RAPD analysis
The serranid subfamily {Epinephelus genus), popularly known as grouper, is one of the most economically important fishes in Southeast Asian countries. In this study, RAPD in association with PCR was employed to determine the genetic variability and to establish the RAPD fingerprinting of groupers...
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my-umt-ir.-6482016-03-10T07:44:16Z Studies on genetic variability of groupers (Epinephelus spp.) from Indo-Malaysian waters using PCR-RAPD analysis Parenrengi, Andi The serranid subfamily {Epinephelus genus), popularly known as grouper, is one of the most economically important fishes in Southeast Asian countries. In this study, RAPD in association with PCR was employed to determine the genetic variability and to establish the RAPD fingerprinting of groupers {Epinephelus spp.). A total of 100 individuals of E. tauvina from six populations taken from Indo-Malaysian waters and 36 individuals of E. merra and E. areolatus taken from Makassar Strait, South Sulawesi were studied. Three tissue preservatives and four DNA extraction techniques were employed to find the best preservative and DNA extraction technique for grouper. A series of optimization experiments of PCR-RAPD protocol were conducted. Out of the 34 screened random primers, ten primers were selected for further analysis. Fakulti Sains dan Teknologi 2001-05 Thesis en http://hdl.handle.net/123456789/648 http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/648/3/QL638%20.S48%20P3%202001%20Abstract.pdf a272ac7d8e380ad2199357a2c016f2de http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/648/4/QL638%20.S48%20P3%202001.pdf 0919d9695526186a08daa5f23d9a981a http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/648/2/license.txt 8a4605be74aa9ea9d79846c1fba20a33 http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/648/5/QL638%20.S48%20P3%202001%20Abstract.pdf.txt 0ff8137e35832b4c6bdbd98228a4f86c http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/648/6/QL638%20.S48%20P3%202001.pdf.txt 04674a3e7234bf1dd66ed5741ff584d9 QL 638 .S48 P3 2001 Parenrengi, Andi Studies on genetic variability of groupers (Epinephelus spp.) from Indo-Malaysian waters using PCR-RAPD analysis Groupers -- Malaysia Serranidae |
| institution |
Universiti Malaysia Terengganu |
| collection |
UMT Repository System |
| language |
English |
| topic |
QL 638 .S48 P3 2001 QL 638 .S48 P3 2001 Studies on genetic variability of groupers (Epinephelus spp.) from Indo-Malaysian waters using PCR-RAPD analysis Groupers -- Malaysia Serranidae |
| spellingShingle |
QL 638 .S48 P3 2001 QL 638 .S48 P3 2001 Studies on genetic variability of groupers (Epinephelus spp.) from Indo-Malaysian waters using PCR-RAPD analysis Groupers -- Malaysia Serranidae Parenrengi, Andi Studies on genetic variability of groupers (Epinephelus spp.) from Indo-Malaysian waters using PCR-RAPD analysis |
| description |
The serranid subfamily {Epinephelus genus), popularly known as grouper, is one of
the most economically important fishes in Southeast Asian countries. In this study,
RAPD in association with PCR was employed to determine the genetic variability
and to establish the RAPD fingerprinting of groupers {Epinephelus spp.). A total of
100 individuals of E. tauvina from six populations taken from Indo-Malaysian waters
and 36 individuals of E. merra and E. areolatus taken from Makassar Strait, South
Sulawesi were studied. Three tissue preservatives and four DNA extraction
techniques were employed to find the best preservative and DNA extraction
technique for grouper. A series of optimization experiments of PCR-RAPD protocol
were conducted. Out of the 34 screened random primers, ten primers were selected
for further analysis. |
| format |
Thesis |
| author |
Parenrengi, Andi |
| author_facet |
Parenrengi, Andi |
| author_sort |
Parenrengi, Andi |
| title |
Studies on genetic variability of groupers (Epinephelus spp.) from Indo-Malaysian waters using PCR-RAPD analysis |
| title_short |
Studies on genetic variability of groupers (Epinephelus spp.) from Indo-Malaysian waters using PCR-RAPD analysis |
| title_full |
Studies on genetic variability of groupers (Epinephelus spp.) from Indo-Malaysian waters using PCR-RAPD analysis |
| title_fullStr |
Studies on genetic variability of groupers (Epinephelus spp.) from Indo-Malaysian waters using PCR-RAPD analysis |
| title_full_unstemmed |
Studies on genetic variability of groupers (Epinephelus spp.) from Indo-Malaysian waters using PCR-RAPD analysis |
| title_sort |
studies on genetic variability of groupers (epinephelus spp.) from indo-malaysian waters using pcr-rapd analysis |
| granting_institution |
Fakulti Sains dan Teknologi |
| url |
http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/648/3/QL638%20.S48%20P3%202001%20Abstract.pdf http://umt-ir.umt.edu.my:8080/jspui/bitstream/123456789/648/4/QL638%20.S48%20P3%202001.pdf |
| _version_ |
1747835802692354048 |