Antioxidative properties of Diplazium esculentum extract by using pressurized hot water extractor: Optimization study

Pressurized hot water extraction (PHWE) was implemented in attempt to reduce the use of toxic organic solvent in extracting bioactive compounds from Diplazium esculentum. Comparison extraction methods were done and the results shows that the PHWE had higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) free...

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Main Author:	Nur Azirah, Jamial
Format:	Thesis
Language:	English
Subjects:	Pressurized hot water extraction (PHWE) Diplazium esculentum Herbal medicines Antioxidant activity
Online Access:	http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/20814/1/p.1-24.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/20814/2/Full%20Text.pdf
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spelling	my-unimap-208142012-09-04T03:23:01Z Antioxidative properties of Diplazium esculentum extract by using pressurized hot water extractor: Optimization study Nur Azirah, Jamial Pressurized hot water extraction (PHWE) was implemented in attempt to reduce the use of toxic organic solvent in extracting bioactive compounds from Diplazium esculentum. Comparison extraction methods were done and the results shows that the PHWE had higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity (70%) and total phenolic contents (118.83f.lg catechin equivalentlmg dry samples) c,.ompared to autoclaving, boiling, soaking, sonicating and Soxhlet extraction. Soaking extraction had the highest total flavonoids contents (28.03f.lg rutin equivalentlmg dry extract). By applying Box-Behnken design of response surface methodology, the optimized condition for the best antioxidant activity of PH WE was at 17SoC, 21 minutes extraction time and SOmL water volume added to 2g of dried ground D. esculentum. The DPPH antiradical efficiency was analyzed where the D. esculentum extracts had ECso value of 1241.14f.lg/mL and time to reach steady state (TEC50) was 79.83 minutes. Thus, the extract was categorized as slow in scavenging the DPPH free radicals. Through HPLC, the identified flavonoids in the crude extracts were quercetin and myricetin. Universiti Malaysia Perlis (UniMAP) 2010 Thesis en http://dspace.unimap.edu.my/123456789/20814 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/20814/1/p.1-24.pdf 8f2caf82e5266a480c59d6904ce06c21 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/20814/2/Full%20Text.pdf 249b2d60cc6ac957398b1ea259965569 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/20814/3/license.txt 71f755f651db4f040d312f80be7b00a5 Pressurized hot water extraction (PHWE) Diplazium esculentum Herbal medicines Antioxidant activity School of Bioprocess Engineering
institution	Universiti Malaysia Perlis
collection	UniMAP Institutional Repository
language	English
topic	Pressurized hot water extraction (PHWE) Diplazium esculentum Herbal medicines Antioxidant activity
spellingShingle	Pressurized hot water extraction (PHWE) Diplazium esculentum Herbal medicines Antioxidant activity Nur Azirah, Jamial Antioxidative properties of Diplazium esculentum extract by using pressurized hot water extractor: Optimization study
description	Pressurized hot water extraction (PHWE) was implemented in attempt to reduce the use of toxic organic solvent in extracting bioactive compounds from Diplazium esculentum. Comparison extraction methods were done and the results shows that the PHWE had higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity (70%) and total phenolic contents (118.83f.lg catechin equivalentlmg dry samples) c,.ompared to autoclaving, boiling, soaking, sonicating and Soxhlet extraction. Soaking extraction had the highest total flavonoids contents (28.03f.lg rutin equivalentlmg dry extract). By applying Box-Behnken design of response surface methodology, the optimized condition for the best antioxidant activity of PH WE was at 17SoC, 21 minutes extraction time and SOmL water volume added to 2g of dried ground D. esculentum. The DPPH antiradical efficiency was analyzed where the D. esculentum extracts had ECso value of 1241.14f.lg/mL and time to reach steady state (TEC50) was 79.83 minutes. Thus, the extract was categorized as slow in scavenging the DPPH free radicals. Through HPLC, the identified flavonoids in the crude extracts were quercetin and myricetin.
format	Thesis
author	Nur Azirah, Jamial
author_facet	Nur Azirah, Jamial
author_sort	Nur Azirah, Jamial
title	Antioxidative properties of Diplazium esculentum extract by using pressurized hot water extractor: Optimization study
title_short	Antioxidative properties of Diplazium esculentum extract by using pressurized hot water extractor: Optimization study
title_full	Antioxidative properties of Diplazium esculentum extract by using pressurized hot water extractor: Optimization study
title_fullStr	Antioxidative properties of Diplazium esculentum extract by using pressurized hot water extractor: Optimization study
title_full_unstemmed	Antioxidative properties of Diplazium esculentum extract by using pressurized hot water extractor: Optimization study
title_sort	antioxidative properties of diplazium esculentum extract by using pressurized hot water extractor: optimization study
granting_institution	Universiti Malaysia Perlis (UniMAP)
granting_department	School of Bioprocess Engineering
url	http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/20814/1/p.1-24.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/20814/2/Full%20Text.pdf
_version_	1747836770579382272

Antioxidative properties of Diplazium esculentum extract by using pressurized hot water extractor: Optimization study

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