Characterization of molecularly imprinted polymer (MIP) for an extraction Of curcumin from Curcuma longa (Turmeric)
A molecularly imprinted polymer (MIP) for selective extraction of curcumin has been synthesized via non-covalent approach by using curcumin as a template. Polymerization was prepared using 2 (two) functional monomers namely methacrylic acid (MAA) and acrylamide (AM) together with 3 (three) differ...
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| Format: | Thesis |
| Language: | English |
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| Online Access: | http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/21606/1/Full%20text.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/21606/2/p.%201-24.pdf |
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| Summary: | A molecularly imprinted polymer (MIP) for selective extraction of curcumin has been
synthesized via non-covalent approach by using curcumin as a template. Polymerization
was prepared using 2 (two) functional monomers namely methacrylic acid (MAA) and
acrylamide (AM) together with 3 (three) different porogens namely chloroform (CHCl3),
tetrahydrofuran (THF) and acetonitrile (MeCN). Porosity and surface area analysis
revealed that the polymer prepared using THF as porogen has the highest average pore
diameter size i.e. 618.43 nm. Batch binding analysis revealed that the largest imprinting
factor was attained by the polymer prepared using MAA and THF as functional monomer
and porogen respectively. Further characterization was carried out using 3 (three)
isotherm models namely Langmuir (LI), Freundlich (FI) and Langmuir-Freundlich (LF-I)
isotherm. The unknown parameters in each isotherm were calculated by using Solver
function in Microsoft Excel and were optimized for R2 value. The calculated R2 values
were found to be (0.91), (0.69) and (0.96) for LI , FI and LFI respectively.. Hence, LFI
was further used to calculate the binding sites (N) and homogeneity (m) of both the MIP
and NIP (non-imprinted polymer). The result showed that MIP1 has more Nt (1250.62
µg/g) as compared to NIP ( 998.35 πg/g) suggesting that MIP has more binding sites and
selective towards curcumin. A 150 mg of polymer mass was packed into SPE (solid
phase extraction) cartridge and subsequently used to extract curcumin from raw turmeric
extract. The recoveries were 43.10% for MIP as compared to 13.46% for NIP. This
suggested that the MIP cartridge exhibited significant selectivity toward curcumin, with
recoveries 67.76% and 39.86% for NIP, indicating that the synthesized MIP has the
potential for curcumin purification through SPE. |
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