Bovine adipose tissue derived stem cells bioengineering: isolation, characterization and differentiation studies

There is a significant potential for stem cells to be exploited for regenerative medicine. Stem cells act as a repair system for the body by differentiating into specialised cells and replenishing cells in regenerative organs such as skin or intestinal tissues. Conventionally in the current cell the...

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Main Author:	Fhataheya, Buang
Format:	Thesis
Language:	English
Subjects:	Stem cells Regenerative medicine Bioengineering Stem cell isolation Bovine Human Stem cells > Research
Online Access:	http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/31295/1/Page%201-24.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/31295/2/Full%20text.pdf
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spelling	my-unimap-312952014-01-19T06:28:22Z Bovine adipose tissue derived stem cells bioengineering: isolation, characterization and differentiation studies Fhataheya, Buang There is a significant potential for stem cells to be exploited for regenerative medicine. Stem cells act as a repair system for the body by differentiating into specialised cells and replenishing cells in regenerative organs such as skin or intestinal tissues. Conventionally in the current cell therapy, the renewed, differentiated cells were mostly sourced form bone marrow mesenchymal stem cell (MSCs) yielding low number of cells and involved a painful procedure under general anaesthesia. In tissue engineering, bioprocessing stem cells are crucial thus, stem cells from adipose tissue were revealed as a new promising source of MSCs. Abundant source of stromal vascular fraction (SVF) needs a good bioprocessing tools for an optimize culture. Isolation of bovine adipose tissue derived stem cells (ASCs) are found to better perform in 0.075% collagenase Type 1 and agitated for 2 hours from the experiment carried out. The static cultures were best inoculated at 1.0 x 104 cells/ml in 6 well plate. The suspension cultures were cultured best in 200rpm, 5ml DMEM: F12 / MesenPRO RS™ at 1.0 x 104 cells/ml in 50ml tube. Suspension (3D) culture of sphere aggregates yield cells density of 1.07 x 106 ASCs upon culture compared to static (2D) culture of 5 x 105 ASCs on average. However the viability of static culture are found to be more superior to suspension culture at high volume, agitation and inoculation sizes suggesting that the parameter are an unfavourable environment to culture in 50ml tubes. Successful differentiation of osteoblast cells were evaluated via Fourier Transform Infra-Red (FTIR) and conventional Van Kossa staining. Simulation of suspension (3D) culture via Computational Fluid Dynamic (CFD) using Volume of Fluid (VOF) model assist in developing an optimised ASCs culture protocol vis-à-vis conventional static (2D) culturing in flask. Universiti Malaysia Perlis (UniMAP) 2013 Thesis en http://dspace.unimap.edu.my:80/dspace/handle/123456789/31295 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/31295/1/Page%201-24.pdf 474b0a3a19e2b0e0e5af79fef56c00e5 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/31295/2/Full%20text.pdf c3b9bcee4d2903ada174edbc15924b26 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/31295/3/license.txt 8a4605be74aa9ea9d79846c1fba20a33 Stem cells Regenerative medicine Bioengineering Stem cell isolation Bovine Human Stem cells --Research School of Bioprocess Engineering
institution	Universiti Malaysia Perlis
collection	UniMAP Institutional Repository
language	English
topic	Stem cells Regenerative medicine Bioengineering Stem cell isolation Bovine Human Stem cells --Research
spellingShingle	Stem cells Regenerative medicine Bioengineering Stem cell isolation Bovine Human Stem cells --Research Fhataheya, Buang Bovine adipose tissue derived stem cells bioengineering: isolation, characterization and differentiation studies
description	There is a significant potential for stem cells to be exploited for regenerative medicine. Stem cells act as a repair system for the body by differentiating into specialised cells and replenishing cells in regenerative organs such as skin or intestinal tissues. Conventionally in the current cell therapy, the renewed, differentiated cells were mostly sourced form bone marrow mesenchymal stem cell (MSCs) yielding low number of cells and involved a painful procedure under general anaesthesia. In tissue engineering, bioprocessing stem cells are crucial thus, stem cells from adipose tissue were revealed as a new promising source of MSCs. Abundant source of stromal vascular fraction (SVF) needs a good bioprocessing tools for an optimize culture. Isolation of bovine adipose tissue derived stem cells (ASCs) are found to better perform in 0.075% collagenase Type 1 and agitated for 2 hours from the experiment carried out. The static cultures were best inoculated at 1.0 x 104 cells/ml in 6 well plate. The suspension cultures were cultured best in 200rpm, 5ml DMEM: F12 / MesenPRO RS™ at 1.0 x 104 cells/ml in 50ml tube. Suspension (3D) culture of sphere aggregates yield cells density of 1.07 x 106 ASCs upon culture compared to static (2D) culture of 5 x 105 ASCs on average. However the viability of static culture are found to be more superior to suspension culture at high volume, agitation and inoculation sizes suggesting that the parameter are an unfavourable environment to culture in 50ml tubes. Successful differentiation of osteoblast cells were evaluated via Fourier Transform Infra-Red (FTIR) and conventional Van Kossa staining. Simulation of suspension (3D) culture via Computational Fluid Dynamic (CFD) using Volume of Fluid (VOF) model assist in developing an optimised ASCs culture protocol vis-à-vis conventional static (2D) culturing in flask.
format	Thesis
author	Fhataheya, Buang
author_facet	Fhataheya, Buang
author_sort	Fhataheya, Buang
title	Bovine adipose tissue derived stem cells bioengineering: isolation, characterization and differentiation studies
title_short	Bovine adipose tissue derived stem cells bioengineering: isolation, characterization and differentiation studies
title_full	Bovine adipose tissue derived stem cells bioengineering: isolation, characterization and differentiation studies
title_fullStr	Bovine adipose tissue derived stem cells bioengineering: isolation, characterization and differentiation studies
title_full_unstemmed	Bovine adipose tissue derived stem cells bioengineering: isolation, characterization and differentiation studies
title_sort	bovine adipose tissue derived stem cells bioengineering: isolation, characterization and differentiation studies
granting_institution	Universiti Malaysia Perlis (UniMAP)
granting_department	School of Bioprocess Engineering
url	http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/31295/1/Page%201-24.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/31295/2/Full%20text.pdf
_version_	1747836789692825600

Bovine adipose tissue derived stem cells bioengineering: isolation, characterization and differentiation studies

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