Studies on the third generation of Ionic liquids - Halophillic Cellulase system for Lignocellulose hydrolysis
The conversion of lignocellulosic biomass into value-added products requires pretreatment, hydrolysis (saccharification) and the conversion of simple sugar into end products. The limiting factor of these three processes lies in the pre-treatment steps. Conventional pre-treatment methods normally u...
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| 格式: | Thesis |
| 语言: | English |
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| 在线阅读: | http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/59825/1/Page%201-24.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/59825/2/Full%20text.pdf |
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| 总结: | The conversion of lignocellulosic biomass into value-added products requires pretreatment, hydrolysis (saccharification) and the conversion of simple sugar into end
products. The limiting factor of these three processes lies in the pre-treatment steps.
Conventional pre-treatment methods normally use strong acids or alkali which pose
environmental problems. Recently pre-treatment using the third generation of Ionic
Liquids (ILs), also known as Deep Eutectic Solvents (DESs) has been considered green
and cost-effective. However, the main drawback of DESs pre-treatment is that it is not fully compatible with the enzyme required in saccharification. This compatibility issue
was addressed in the present study by producing a salt tolerant enzyme (halophilic
enzyme) and formulating low viscosity, thermal stable DES. The halophilic enzyme was
produced from a newly isolated halophilic fungus, identified as Aspergillus terreus
UniMAP AA-6; DESs were synthesized and screened using choline chloride as a
hydrogen bond acceptor and selected carboxylic acids and polyol alcohols as hydrogen
bond donors. The compatibility between halophilic cellulase and DESs was evaluated
by monitoring the stability of halophilic cellulase in the presence of various
concentrations of commercial ILs and DESs. The applicability of the DES-cellulase
system for lignocelluloses hydrolysis was evaluated based on glucose production,
energy consumption and kinetic performance. It was found that halophilic cellulase
showed higher stability in the presence of 10% (v/v) ILs and also was stable and
retained 90 % of its original activity in the presence of 10% (v/v) DESs. The DESs-
Cellulase system exhibited higher glucose percentage enhancement and lower energy
consumption as compared to diluted alkali system, while in terms of kinetic
performance, DES exhibited good kinetic performance, which reflects the ability of
DESs to serve as good saccharification media for the DESs-cellulase system. Finally,
with regard to glucose production, rice husk treated with DESs-halophilic cellulase
system were as good as the typical IL pre-treatment method but with extra benefits in
terms of cost and environmental aspects. These findings have demonstrated a better
approach for the in situ saccharification of DESs pre-treated lignocelluloses. |
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