Studies on the third generation of Ionic liquids - Halophillic Cellulase system for Lignocellulose hydrolysis
The conversion of lignocellulosic biomass into value-added products requires pretreatment, hydrolysis (saccharification) and the conversion of simple sugar into end products. The limiting factor of these three processes lies in the pre-treatment steps. Conventional pre-treatment methods normally u...
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my-unimap-598252023-04-04T02:15:36Z Studies on the third generation of Ionic liquids - Halophillic Cellulase system for Lignocellulose hydrolysis Ahmad Anas, Nagoor Gunny Assoc. Prof. Dr. Dachyar Arbain The conversion of lignocellulosic biomass into value-added products requires pretreatment, hydrolysis (saccharification) and the conversion of simple sugar into end products. The limiting factor of these three processes lies in the pre-treatment steps. Conventional pre-treatment methods normally use strong acids or alkali which pose environmental problems. Recently pre-treatment using the third generation of Ionic Liquids (ILs), also known as Deep Eutectic Solvents (DESs) has been considered green and cost-effective. However, the main drawback of DESs pre-treatment is that it is not fully compatible with the enzyme required in saccharification. This compatibility issue was addressed in the present study by producing a salt tolerant enzyme (halophilic enzyme) and formulating low viscosity, thermal stable DES. The halophilic enzyme was produced from a newly isolated halophilic fungus, identified as Aspergillus terreus UniMAP AA-6; DESs were synthesized and screened using choline chloride as a hydrogen bond acceptor and selected carboxylic acids and polyol alcohols as hydrogen bond donors. The compatibility between halophilic cellulase and DESs was evaluated by monitoring the stability of halophilic cellulase in the presence of various concentrations of commercial ILs and DESs. The applicability of the DES-cellulase system for lignocelluloses hydrolysis was evaluated based on glucose production, energy consumption and kinetic performance. It was found that halophilic cellulase showed higher stability in the presence of 10% (v/v) ILs and also was stable and retained 90 % of its original activity in the presence of 10% (v/v) DESs. The DESs- Cellulase system exhibited higher glucose percentage enhancement and lower energy consumption as compared to diluted alkali system, while in terms of kinetic performance, DES exhibited good kinetic performance, which reflects the ability of DESs to serve as good saccharification media for the DESs-cellulase system. Finally, with regard to glucose production, rice husk treated with DESs-halophilic cellulase system were as good as the typical IL pre-treatment method but with extra benefits in terms of cost and environmental aspects. These findings have demonstrated a better approach for the in situ saccharification of DESs pre-treated lignocelluloses. Universiti Malaysia Perlis (UniMAP) 2015 Thesis en http://dspace.unimap.edu.my:80/xmlui/handle/123456789/59825 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/59825/1/Page%201-24.pdf 95bc629d243ca474fcee207abea56ddf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/59825/2/Full%20text.pdf f430f6fb576408eabcf68a9a984ee75b http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/59825/3/license.txt 8a4605be74aa9ea9d79846c1fba20a33 Lignocelluloses Lignocellulosic biomass Biomass Ionic Liquids (ILs) Lignocellulosic biomass -- Product School of Bioprocess Engineering |
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Universiti Malaysia Perlis |
collection |
UniMAP Institutional Repository |
language |
English |
advisor |
Assoc. Prof. Dr. Dachyar Arbain |
topic |
Lignocelluloses Lignocellulosic biomass Biomass Ionic Liquids (ILs) Lignocellulosic biomass -- Product |
spellingShingle |
Lignocelluloses Lignocellulosic biomass Biomass Ionic Liquids (ILs) Lignocellulosic biomass -- Product Ahmad Anas, Nagoor Gunny Studies on the third generation of Ionic liquids - Halophillic Cellulase system for Lignocellulose hydrolysis |
description |
The conversion of lignocellulosic biomass into value-added products requires pretreatment, hydrolysis (saccharification) and the conversion of simple sugar into end
products. The limiting factor of these three processes lies in the pre-treatment steps.
Conventional pre-treatment methods normally use strong acids or alkali which pose
environmental problems. Recently pre-treatment using the third generation of Ionic
Liquids (ILs), also known as Deep Eutectic Solvents (DESs) has been considered green
and cost-effective. However, the main drawback of DESs pre-treatment is that it is not fully compatible with the enzyme required in saccharification. This compatibility issue
was addressed in the present study by producing a salt tolerant enzyme (halophilic
enzyme) and formulating low viscosity, thermal stable DES. The halophilic enzyme was
produced from a newly isolated halophilic fungus, identified as Aspergillus terreus
UniMAP AA-6; DESs were synthesized and screened using choline chloride as a
hydrogen bond acceptor and selected carboxylic acids and polyol alcohols as hydrogen
bond donors. The compatibility between halophilic cellulase and DESs was evaluated
by monitoring the stability of halophilic cellulase in the presence of various
concentrations of commercial ILs and DESs. The applicability of the DES-cellulase
system for lignocelluloses hydrolysis was evaluated based on glucose production,
energy consumption and kinetic performance. It was found that halophilic cellulase
showed higher stability in the presence of 10% (v/v) ILs and also was stable and
retained 90 % of its original activity in the presence of 10% (v/v) DESs. The DESs-
Cellulase system exhibited higher glucose percentage enhancement and lower energy
consumption as compared to diluted alkali system, while in terms of kinetic
performance, DES exhibited good kinetic performance, which reflects the ability of
DESs to serve as good saccharification media for the DESs-cellulase system. Finally,
with regard to glucose production, rice husk treated with DESs-halophilic cellulase
system were as good as the typical IL pre-treatment method but with extra benefits in
terms of cost and environmental aspects. These findings have demonstrated a better
approach for the in situ saccharification of DESs pre-treated lignocelluloses. |
format |
Thesis |
author |
Ahmad Anas, Nagoor Gunny |
author_facet |
Ahmad Anas, Nagoor Gunny |
author_sort |
Ahmad Anas, Nagoor Gunny |
title |
Studies on the third generation of Ionic liquids - Halophillic Cellulase system for Lignocellulose hydrolysis |
title_short |
Studies on the third generation of Ionic liquids - Halophillic Cellulase system for Lignocellulose hydrolysis |
title_full |
Studies on the third generation of Ionic liquids - Halophillic Cellulase system for Lignocellulose hydrolysis |
title_fullStr |
Studies on the third generation of Ionic liquids - Halophillic Cellulase system for Lignocellulose hydrolysis |
title_full_unstemmed |
Studies on the third generation of Ionic liquids - Halophillic Cellulase system for Lignocellulose hydrolysis |
title_sort |
studies on the third generation of ionic liquids - halophillic cellulase system for lignocellulose hydrolysis |
granting_institution |
Universiti Malaysia Perlis (UniMAP) |
granting_department |
School of Bioprocess Engineering |
url |
http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/59825/1/Page%201-24.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/59825/2/Full%20text.pdf |
_version_ |
1776104219880718336 |