Green synthesis of silver nanoparticles using Piper Betle leaf extracts and their antimicrobial properties against isolated Phyto-pathogen model

Silver nanoparticles are well known as one of the promising agent especially for antibacterial activity. This project was aimed to evaluate the synthesized silver nanoparticles from Piper betle and their antimicrobial properties against phyto-pathogen model. The formation of silver nanoparticles i...

Full description

Saved in:

Bibliographic Details
Main Author:	Nooramalina, Azhar
Format:	Thesis
Language:	English
Subjects:	Silver nanoparticles Piper betle Antimicrobial Antimicrobial properties Phyto-pathogen model
Online Access:	http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/59928/1/p.1-24..pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/59928/2/Full%20Text.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-unimap-59928
record_format	uketd_dc
spelling	my-unimap-599282023-04-04T02:13:17Z Green synthesis of silver nanoparticles using Piper Betle leaf extracts and their antimicrobial properties against isolated Phyto-pathogen model Nooramalina, Azhar Dr. Khairul Farihan Kasim Silver nanoparticles are well known as one of the promising agent especially for antibacterial activity. This project was aimed to evaluate the synthesized silver nanoparticles from Piper betle and their antimicrobial properties against phyto-pathogen model. The formation of silver nanoparticles in aqueous extract were confirmed by colour changes from yellowish to brown. To further confirm the presence of silver nanoparticles, UV-Vis spectrum was used and the absorbance of the silver nanoparticles were analysed at 452 nm. Antimicrobial activity of the synthesized silver nanoparticles of P. bettle was evaluated by using agar well plate on Escherichia coli (gram-negative bacteria), Pseudomonas aeruginasa (gram-positive bacteria) and the Aspergillus niger (fungi). The results show that synthesized silver nanoparticles on 1 mM concentration exhibits admirable zone of inhibition against E. coli, P. aeruginasa and A. niger (14.1±0.13, 14.5±0.17 and 14.7±0.40 mm, respectively). All the synthesized silver nanoparticles show significant antimicrobial activity compared to the aqueous extract of P. bettle. Minimum inhibition concentration (MIC) of the best synthesized silver nanoparticles of P. bettle (1 Mm) was further evaluated and shows that P. aeruginasa, E. coli and A. niger was perceived at three-fold, two-fold and one-fold dilution from the original samples respectively. In order to characterize the size and shape of silver nanoparticles, field emission scanning electron microscope (FESEM) was used and revealed that silver nanoparticles were in spherical shape that range from 15 to 19 nm in size. The presence of elemental silver was obtained by using energy-dispersive spectroscopy (EDX), which Universiti Malaysia Perlis (UniMAP) 2017-06 Thesis en http://dspace.unimap.edu.my:80/xmlui/handle/123456789/59928 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/59928/1/p.1-24..pdf b73d26c759dccb39bb8f907eb170e3bd http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/59928/2/Full%20Text.pdf a72487301bc2ecf3275485a07531bf9b http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/59928/3/license.txt 8a4605be74aa9ea9d79846c1fba20a33 Silver nanoparticles Piper betle Antimicrobial Antimicrobial properties Phyto-pathogen model School of Bioprocess Engineering
institution	Universiti Malaysia Perlis
collection	UniMAP Institutional Repository
language	English
advisor	Dr. Khairul Farihan Kasim
topic	Silver nanoparticles Piper betle Antimicrobial Antimicrobial properties Phyto-pathogen model
spellingShingle	Silver nanoparticles Piper betle Antimicrobial Antimicrobial properties Phyto-pathogen model Nooramalina, Azhar Green synthesis of silver nanoparticles using Piper Betle leaf extracts and their antimicrobial properties against isolated Phyto-pathogen model
description	Silver nanoparticles are well known as one of the promising agent especially for antibacterial activity. This project was aimed to evaluate the synthesized silver nanoparticles from Piper betle and their antimicrobial properties against phyto-pathogen model. The formation of silver nanoparticles in aqueous extract were confirmed by colour changes from yellowish to brown. To further confirm the presence of silver nanoparticles, UV-Vis spectrum was used and the absorbance of the silver nanoparticles were analysed at 452 nm. Antimicrobial activity of the synthesized silver nanoparticles of P. bettle was evaluated by using agar well plate on Escherichia coli (gram-negative bacteria), Pseudomonas aeruginasa (gram-positive bacteria) and the Aspergillus niger (fungi). The results show that synthesized silver nanoparticles on 1 mM concentration exhibits admirable zone of inhibition against E. coli, P. aeruginasa and A. niger (14.1±0.13, 14.5±0.17 and 14.7±0.40 mm, respectively). All the synthesized silver nanoparticles show significant antimicrobial activity compared to the aqueous extract of P. bettle. Minimum inhibition concentration (MIC) of the best synthesized silver nanoparticles of P. bettle (1 Mm) was further evaluated and shows that P. aeruginasa, E. coli and A. niger was perceived at three-fold, two-fold and one-fold dilution from the original samples respectively. In order to characterize the size and shape of silver nanoparticles, field emission scanning electron microscope (FESEM) was used and revealed that silver nanoparticles were in spherical shape that range from 15 to 19 nm in size. The presence of elemental silver was obtained by using energy-dispersive spectroscopy (EDX), which
format	Thesis
author	Nooramalina, Azhar
author_facet	Nooramalina, Azhar
author_sort	Nooramalina, Azhar
title	Green synthesis of silver nanoparticles using Piper Betle leaf extracts and their antimicrobial properties against isolated Phyto-pathogen model
title_short	Green synthesis of silver nanoparticles using Piper Betle leaf extracts and their antimicrobial properties against isolated Phyto-pathogen model
title_full	Green synthesis of silver nanoparticles using Piper Betle leaf extracts and their antimicrobial properties against isolated Phyto-pathogen model
title_fullStr	Green synthesis of silver nanoparticles using Piper Betle leaf extracts and their antimicrobial properties against isolated Phyto-pathogen model
title_full_unstemmed	Green synthesis of silver nanoparticles using Piper Betle leaf extracts and their antimicrobial properties against isolated Phyto-pathogen model
title_sort	green synthesis of silver nanoparticles using piper betle leaf extracts and their antimicrobial properties against isolated phyto-pathogen model
granting_institution	Universiti Malaysia Perlis (UniMAP)
granting_department	School of Bioprocess Engineering
url	http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/59928/1/p.1-24..pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/59928/2/Full%20Text.pdf
_version_	1776104220123987968

Green synthesis of silver nanoparticles using Piper Betle leaf extracts and their antimicrobial properties against isolated Phyto-pathogen model

Similar Items