Utilization of Gracilaria sp. liquid for bioethanol production

Seaweed liquid from filter-pressed drying process of Gracilaria sp. has caused unpleasant odour to the environment. Although this liquid can partially be used as biofertilizer and heavy metals adsorbent, a bigger portion of the liquid remain unutilized thus causing problem to environment. The pres...

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Main Author:	Nur Zatul ‘Iffah, Zakaria
Format:	Thesis
Language:	English
Subjects:	Gracilaria Bioethanol production Marine algae Seaweed
Online Access:	http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/61544/1/Page%201-24.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/61544/2/Full%20text.pdf
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id	my-unimap-61544
record_format	uketd_dc
spelling	my-unimap-615442023-04-04T02:10:38Z Utilization of Gracilaria sp. liquid for bioethanol production Nur Zatul ‘Iffah, Zakaria Assoc. Prof. Dr. Dachyar Arbain Seaweed liquid from filter-pressed drying process of Gracilaria sp. has caused unpleasant odour to the environment. Although this liquid can partially be used as biofertilizer and heavy metals adsorbent, a bigger portion of the liquid remain unutilized thus causing problem to environment. The present study was aimed at utilizing Gracilaria sp. liquid either as ethanol feedstock or supplement in fermentation media. To achieve this aim, Saccharomyces cerevisiae was used as a benchmark. Some locally isolated strains have been screened, isolated and tested their efficacy in fermenting Gracilaria sp. liquid to produce ethanol. Prior to fermentation, the liquid was pretreated using thermal dilute sulphuric acid hydrolysis in sequence to increase reducing sugar concentration and its conditions were statistically optimized by using Response Surface Methodology (RSM) method. The optimum condition of thermal dilute acid hydrolysis obtained was 0.3 M sulphuric acid, 0.7 g/ml of substrate and 120 oC. Nevertheless, all the potential strains isolated in this study produced lower yield as compared to S.cerevisiae. Therefore, this yeast was used in subsequent experiments. However, when used as a supplement in the fermentation media to produce ethanol using S.cerevisiae, the liquid was able to boost the ethanol production three folds from 200 mg/L of 2 % glucose alone to 600 mg/L of 2% glucose in the liquid. Additionally, the Gracilaria sp. liquid was able to substitute yeast extract and peptone within Yeast Potato Dextrose (YPD) media to produce 647.48 mg/l ethanol as compared to only 542.39 mg/l with normal YPD which contains yeast extract and peptone. For that reason, utilization of Gracilaria sp. liquid as the supplement in the medium should be considered because it is capable to enhance ethanol production without addition of other nutrients. Universiti Malaysia Perlis (UniMAP) 2015 Thesis en http://dspace.unimap.edu.my:80/xmlui/handle/123456789/61544 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/61544/1/Page%201-24.pdf 1551b909954df1698e11c031e40eacba http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/61544/2/Full%20text.pdf a83c197b26d6f0c4685eab62a047de49 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/61544/3/license.txt 8a4605be74aa9ea9d79846c1fba20a33 Gracilaria Bioethanol production Marine algae Seaweed School of Bioprocess Engineering
institution	Universiti Malaysia Perlis
collection	UniMAP Institutional Repository
language	English
advisor	Assoc. Prof. Dr. Dachyar Arbain
topic	Gracilaria Bioethanol production Marine algae Seaweed
spellingShingle	Gracilaria Bioethanol production Marine algae Seaweed Nur Zatul ‘Iffah, Zakaria Utilization of Gracilaria sp. liquid for bioethanol production
description	Seaweed liquid from filter-pressed drying process of Gracilaria sp. has caused unpleasant odour to the environment. Although this liquid can partially be used as biofertilizer and heavy metals adsorbent, a bigger portion of the liquid remain unutilized thus causing problem to environment. The present study was aimed at utilizing Gracilaria sp. liquid either as ethanol feedstock or supplement in fermentation media. To achieve this aim, Saccharomyces cerevisiae was used as a benchmark. Some locally isolated strains have been screened, isolated and tested their efficacy in fermenting Gracilaria sp. liquid to produce ethanol. Prior to fermentation, the liquid was pretreated using thermal dilute sulphuric acid hydrolysis in sequence to increase reducing sugar concentration and its conditions were statistically optimized by using Response Surface Methodology (RSM) method. The optimum condition of thermal dilute acid hydrolysis obtained was 0.3 M sulphuric acid, 0.7 g/ml of substrate and 120 oC. Nevertheless, all the potential strains isolated in this study produced lower yield as compared to S.cerevisiae. Therefore, this yeast was used in subsequent experiments. However, when used as a supplement in the fermentation media to produce ethanol using S.cerevisiae, the liquid was able to boost the ethanol production three folds from 200 mg/L of 2 % glucose alone to 600 mg/L of 2% glucose in the liquid. Additionally, the Gracilaria sp. liquid was able to substitute yeast extract and peptone within Yeast Potato Dextrose (YPD) media to produce 647.48 mg/l ethanol as compared to only 542.39 mg/l with normal YPD which contains yeast extract and peptone. For that reason, utilization of Gracilaria sp. liquid as the supplement in the medium should be considered because it is capable to enhance ethanol production without addition of other nutrients.
format	Thesis
author	Nur Zatul ‘Iffah, Zakaria
author_facet	Nur Zatul ‘Iffah, Zakaria
author_sort	Nur Zatul ‘Iffah, Zakaria
title	Utilization of Gracilaria sp. liquid for bioethanol production
title_short	Utilization of Gracilaria sp. liquid for bioethanol production
title_full	Utilization of Gracilaria sp. liquid for bioethanol production
title_fullStr	Utilization of Gracilaria sp. liquid for bioethanol production
title_full_unstemmed	Utilization of Gracilaria sp. liquid for bioethanol production
title_sort	utilization of gracilaria sp. liquid for bioethanol production
granting_institution	Universiti Malaysia Perlis (UniMAP)
granting_department	School of Bioprocess Engineering
url	http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/61544/1/Page%201-24.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/61544/2/Full%20text.pdf
_version_	1776104221084483584

Utilization of Gracilaria sp. liquid for bioethanol production

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