Enzymatic assisted citronella essential oil extraction from Cymbopogan Winterianus

The essential oil of Citronella is commonly extracted via typical steam distillation of citronella plan parts. However, the oil yield is generally low (0.22% - 1.0%), causing burden to many entrepreneurs to meet the strong demand. Therefore, the study was conducted to improve the yield of citronella...

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Format:	Thesis
Language:	English
Subjects:	Citronella oil Steam distillation Essential oil Cymbopogan winterianus
Online Access:	http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/72275/1/Pg1-24.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/72275/2/Full%20text.pdf
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id	my-unimap-72275
record_format	uketd_dc
spelling	my-unimap-722752023-04-04T01:58:00Z Enzymatic assisted citronella essential oil extraction from Cymbopogan Winterianus Arbain, Dachyar, Assoc. Prof. Dr. Mohd Noor, Ahmad, Prof. The essential oil of Citronella is commonly extracted via typical steam distillation of citronella plan parts. However, the oil yield is generally low (0.22% - 1.0%), causing burden to many entrepreneurs to meet the strong demand. Therefore, the study was conducted to improve the yield of citronella oil recovery by introducing an optimised enzymatic assisted pre-treatment using crude microbial enzymes isolated from the soil surrounding the citronella roots. Firstly, the lignocellulosic components of dried citronella roots were investigated to evaluate the most abundance component for selection of enzymes useful for pre-treatment. The composition analysis has identified cellulose as the major lignocellulosic component (38.21%±1.65), followed by hemicellulose (30.49%±0.93), lignin (21.12%±1.86) and extractives (4.97%±0.22). 31 out of 52 isolated strains have been identified as positive cellulase producer. Based on the calculated cellulolytic indexes, 4 strains i.e; UniMAPF7, UniMAPF16, UniMAPF24, and UniMAPF27 were selected for application in the laboratory scale pre-treatment process. Results from the growth curve analysis and enzyme activity study suggested that the optimal time for enzyme harvesting occurred at day 5 for UniMAPF7, day 4 for UniMAPF24 and day 3 for UniMAPF16 and UniMAPF27. Laboratory scale oil extraction of pre-treated citronella root with crude enzymes of UniMAPF7 and UniMAPF24 yielded in a significant 2.5-fold and 1.7-fold increased in citronella oil recovery, respectively, when compared to the untreated citronella root as control. Universiti Malaysia Perlis (UniMAP) Thesis en http://dspace.unimap.edu.my:80/xmlui/handle/123456789/72275 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/72275/3/license.txt 8a4605be74aa9ea9d79846c1fba20a33 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/72275/1/Pg1-24.pdf ff558a1d901dd45e82d6bc342f3aee60 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/72275/2/Full%20text.pdf 1851c8c378062fe5f9a5a04150d6b3c7 Universiti Malaysia Perlis (UniMAP) Citronella oil Steam distillation Essential oil Cymbopogan winterianus School of Bioprocess Engineering
institution	Universiti Malaysia Perlis
collection	UniMAP Institutional Repository
language	English
advisor	Arbain, Dachyar, Assoc. Prof. Dr. Mohd Noor, Ahmad, Prof.
topic	Citronella oil Steam distillation Essential oil Cymbopogan winterianus
spellingShingle	Citronella oil Steam distillation Essential oil Cymbopogan winterianus Enzymatic assisted citronella essential oil extraction from Cymbopogan Winterianus
description	The essential oil of Citronella is commonly extracted via typical steam distillation of citronella plan parts. However, the oil yield is generally low (0.22% - 1.0%), causing burden to many entrepreneurs to meet the strong demand. Therefore, the study was conducted to improve the yield of citronella oil recovery by introducing an optimised enzymatic assisted pre-treatment using crude microbial enzymes isolated from the soil surrounding the citronella roots. Firstly, the lignocellulosic components of dried citronella roots were investigated to evaluate the most abundance component for selection of enzymes useful for pre-treatment. The composition analysis has identified cellulose as the major lignocellulosic component (38.21%±1.65), followed by hemicellulose (30.49%±0.93), lignin (21.12%±1.86) and extractives (4.97%±0.22). 31 out of 52 isolated strains have been identified as positive cellulase producer. Based on the calculated cellulolytic indexes, 4 strains i.e; UniMAPF7, UniMAPF16, UniMAPF24, and UniMAPF27 were selected for application in the laboratory scale pre-treatment process. Results from the growth curve analysis and enzyme activity study suggested that the optimal time for enzyme harvesting occurred at day 5 for UniMAPF7, day 4 for UniMAPF24 and day 3 for UniMAPF16 and UniMAPF27. Laboratory scale oil extraction of pre-treated citronella root with crude enzymes of UniMAPF7 and UniMAPF24 yielded in a significant 2.5-fold and 1.7-fold increased in citronella oil recovery, respectively, when compared to the untreated citronella root as control.
format	Thesis
title	Enzymatic assisted citronella essential oil extraction from Cymbopogan Winterianus
title_short	Enzymatic assisted citronella essential oil extraction from Cymbopogan Winterianus
title_full	Enzymatic assisted citronella essential oil extraction from Cymbopogan Winterianus
title_fullStr	Enzymatic assisted citronella essential oil extraction from Cymbopogan Winterianus
title_full_unstemmed	Enzymatic assisted citronella essential oil extraction from Cymbopogan Winterianus
title_sort	enzymatic assisted citronella essential oil extraction from cymbopogan winterianus
granting_institution	Universiti Malaysia Perlis (UniMAP)
granting_department	School of Bioprocess Engineering
url	http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/72275/1/Pg1-24.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/72275/2/Full%20text.pdf
_version_	1776104221558439936

Enzymatic assisted citronella essential oil extraction from Cymbopogan Winterianus

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