A study on atmospheric pressure plasma jet for hospital acquired bacterial inactivation by different working gases with varying flow rate and exposure time
Atmospheric Pressure Plasma Jet (APPJ) is ionized gas that is generated at atmospheric pressure and at room temperature, by allowing the gas to flow in a small tube with the higher voltage supplied to the area. Plasma offers many advantages that makes it suitable in various applications, including...
Saved in:
| Format: | Thesis |
|---|---|
| Language: | English |
| Subjects: | |
| Online Access: | http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77167/1/Page%201-24.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77167/2/Full%20text.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77167/4/Noor%20Albatyiah.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
my-unimap-77167 |
|---|---|
| record_format |
uketd_dc |
| spelling |
my-unimap-771672022-11-24T06:51:42Z A study on atmospheric pressure plasma jet for hospital acquired bacterial inactivation by different working gases with varying flow rate and exposure time Siti Khadijah, Za'aba, Dr. Atmospheric Pressure Plasma Jet (APPJ) is ionized gas that is generated at atmospheric pressure and at room temperature, by allowing the gas to flow in a small tube with the higher voltage supplied to the area. Plasma offers many advantages that makes it suitable in various applications, including medicine and sterilization. This research focuses on a cold plasma application, namely APPJ, in the sterilization field. The objective of this study is to establish the plasma for the inactivation of bacteria, to study the microbial inactivation effects of APPJ variables in term of the types of microbes, the types of gases, the various gas flow rates, and the exposure times, to study the optical emission spectrum of the APPJ, and to observe the bacteria after exposure to plasma by high power microscope. In this study, a self-developed APPJ was used to inactivate Acinetobacter baumannii (A.baumannii) and Methicillin-resistant Staphylococcus aureus (MRSA) by using different gases, namely He, He/N, and Ar at various gas flow rates in the range of 300ml/min to 1000ml/min, for 9, 12, 15, and 18 seconds of exposure. After that, the treated samples were transferred to a new agar surface and incubated for 24 hours at 37◦C to observe bacterial growth. The A.baumannii and the MRSA were successfully inactivated by the APPJ. The He plasma inactivated the bacteria more effectively than the other gases. The A.baumannii and the MRSA bacteria were fully inactivated after 9 and 12 seconds, respectively, at the flow rate of 1000ml/min. Ar plasma achieved 0% survival at 9s exposure, however the inactivation of bacteria were not consistent especially for MRSA. Universiti Malaysia Perlis (UniMAP) Thesis en http://dspace.unimap.edu.my:80/xmlui/handle/123456789/77167 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77167/3/license.txt 8a4605be74aa9ea9d79846c1fba20a33 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77167/1/Page%201-24.pdf af0133d0216ade0e8520862dafbd1e23 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77167/2/Full%20text.pdf 1d543eb7ed133524cab8fb580df28088 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77167/4/Noor%20Albatyiah.pdf 7bf98dd7eabda2b9a1c1e2ae396b0677 Universiti Malaysia Perlis (UniMAP) Plasma jets Atmospheric Pressure Plasma Jet (APPJ) Ionized gas Plasma School of Mechatronic Engineering |
| institution |
Universiti Malaysia Perlis |
| collection |
UniMAP Institutional Repository |
| language |
English |
| advisor |
Siti Khadijah, Za'aba, Dr. |
| topic |
Plasma jets Atmospheric Pressure Plasma Jet (APPJ) Ionized gas Plasma |
| spellingShingle |
Plasma jets Atmospheric Pressure Plasma Jet (APPJ) Ionized gas Plasma A study on atmospheric pressure plasma jet for hospital acquired bacterial inactivation by different working gases with varying flow rate and exposure time |
| description |
Atmospheric Pressure Plasma Jet (APPJ) is ionized gas that is generated at atmospheric pressure and at room temperature, by allowing the gas to flow in a small tube with the higher voltage supplied to the area. Plasma offers many advantages that makes it
suitable in various applications, including medicine and sterilization. This research focuses on a cold plasma application, namely APPJ, in the sterilization field. The objective of this study is to establish the plasma for the inactivation of bacteria, to study
the microbial inactivation effects of APPJ variables in term of the types of microbes, the types of gases, the various gas flow rates, and the exposure times, to study the optical
emission spectrum of the APPJ, and to observe the bacteria after exposure to plasma by
high power microscope. In this study, a self-developed APPJ was used to inactivate
Acinetobacter baumannii (A.baumannii) and Methicillin-resistant Staphylococcus
aureus (MRSA) by using different gases, namely He, He/N, and Ar at various gas flow
rates in the range of 300ml/min to 1000ml/min, for 9, 12, 15, and 18 seconds of
exposure. After that, the treated samples were transferred to a new agar surface and
incubated for 24 hours at 37◦C to observe bacterial growth. The A.baumannii and the
MRSA were successfully inactivated by the APPJ. The He plasma inactivated the
bacteria more effectively than the other gases. The A.baumannii and the MRSA bacteria
were fully inactivated after 9 and 12 seconds, respectively, at the flow rate of
1000ml/min. Ar plasma achieved 0% survival at 9s exposure, however the inactivation
of bacteria were not consistent especially for MRSA. |
| format |
Thesis |
| title |
A study on atmospheric pressure plasma jet for hospital acquired bacterial inactivation by different working gases with varying flow rate and exposure time |
| title_short |
A study on atmospheric pressure plasma jet for hospital acquired bacterial inactivation by different working gases with varying flow rate and exposure time |
| title_full |
A study on atmospheric pressure plasma jet for hospital acquired bacterial inactivation by different working gases with varying flow rate and exposure time |
| title_fullStr |
A study on atmospheric pressure plasma jet for hospital acquired bacterial inactivation by different working gases with varying flow rate and exposure time |
| title_full_unstemmed |
A study on atmospheric pressure plasma jet for hospital acquired bacterial inactivation by different working gases with varying flow rate and exposure time |
| title_sort |
study on atmospheric pressure plasma jet for hospital acquired bacterial inactivation by different working gases with varying flow rate and exposure time |
| granting_institution |
Universiti Malaysia Perlis (UniMAP) |
| granting_department |
School of Mechatronic Engineering |
| url |
http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77167/1/Page%201-24.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77167/2/Full%20text.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77167/4/Noor%20Albatyiah.pdf |
| _version_ |
1776104233945268224 |