Induction of chlamydospores in pleurotus pulmonarius (grey oyster mushroom) as inoculum and development of lyophilized spawn

P. pulmonarius is cultivated by using spawn inoculated with mycelial cultures obtained from spore or tissue culture. However, the application of spore is prone to contamination while tissue culture application may contribute to somaclonal variation. The mushroom grain spawn can be only stored for 3...

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Format:	Thesis
Language:	English
Subjects:	Cultivated mushroom Pleurotus Lyophilization Pleurotus pulmonarius
Online Access:	http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77869/1/Page%201-24.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77869/2/Full%20text.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77869/4/Sharul%20Aida.pdf
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spelling	my-unimap-778692023-04-04T02:04:57Z Induction of chlamydospores in pleurotus pulmonarius (grey oyster mushroom) as inoculum and development of lyophilized spawn Zarina, Zakaria, Dr. P. pulmonarius is cultivated by using spawn inoculated with mycelial cultures obtained from spore or tissue culture. However, the application of spore is prone to contamination while tissue culture application may contribute to somaclonal variation. The mushroom grain spawn can be only stored for 30 days. Hence, this study was conducted to improve production and preservation technique for P. pulmonarius spawns by using the combination of induce asexual sporulation and lyophilization techniques respectively. The chlamydospore was induced using various stress media namely DGlucose Soluble Starch, D-Glucose CaCl.2H₂O, D-Glucose NaCl, D-Glucose PEG6000, D-Glucose Glycerol, and D-Glucose Na₂SO₄. Three parameters namely; pH, temperature, and incubation period were selected for optimization of chlamydospore formation. It was found that, D-glucose soluble starch stress medium showed the highest 4.5 x107 chlamydospore production with highest chlamydospore production achieved after 78 hours, at pH 6 and 26˚C of temperature by using 1.5 x 107 inoculum size. Next, the spawn was pre-treated with 20% mixture of trehalose and skimmed milk lyoprotectant and lyophilized by using freeze dryer (Labconco) at -40ºC for 2 to 3 days. The spawn was sealed and stored at room temperature for 1 month. After that, the spawn was treated with 2 hours of rehydration treatment prior to inoculation on PDA medium. The chlamydospore-lyophilized spawn was then compared with the chlamydospore spawn which preserved using cryopreservation, sub-culturing, and 4˚C refrigeration techniques in aspect of growth rate and yield. Growth kinetic analysis found that chlamydospore spawn exhibited higher growth rate; 0.24d-1 compared to tissue culture spawn. Universiti Malaysia Perlis (UniMAP) Thesis en http://dspace.unimap.edu.my:80/xmlui/handle/123456789/77869 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77869/3/license.txt 8a4605be74aa9ea9d79846c1fba20a33 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77869/1/Page%201-24.pdf 6ca3f1a727f736e050561a9b845a42d3 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77869/2/Full%20text.pdf c714686797caf6356cd6d9face93f690 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77869/4/Sharul%20Aida.pdf 7d86660e1babe6a53e0c445236e9651e Universiti Malaysia Perlis (UniMAP) Cultivated mushroom Pleurotus Lyophilization Pleurotus pulmonarius School of Bioprocess Engineering
institution	Universiti Malaysia Perlis
collection	UniMAP Institutional Repository
language	English
advisor	Zarina, Zakaria, Dr.
topic	Cultivated mushroom Pleurotus Lyophilization Pleurotus pulmonarius
spellingShingle	Cultivated mushroom Pleurotus Lyophilization Pleurotus pulmonarius Induction of chlamydospores in pleurotus pulmonarius (grey oyster mushroom) as inoculum and development of lyophilized spawn
description	P. pulmonarius is cultivated by using spawn inoculated with mycelial cultures obtained from spore or tissue culture. However, the application of spore is prone to contamination while tissue culture application may contribute to somaclonal variation. The mushroom grain spawn can be only stored for 30 days. Hence, this study was conducted to improve production and preservation technique for P. pulmonarius spawns by using the combination of induce asexual sporulation and lyophilization techniques respectively. The chlamydospore was induced using various stress media namely DGlucose Soluble Starch, D-Glucose CaCl.2H₂O, D-Glucose NaCl, D-Glucose PEG6000, D-Glucose Glycerol, and D-Glucose Na₂SO₄. Three parameters namely; pH, temperature, and incubation period were selected for optimization of chlamydospore formation. It was found that, D-glucose soluble starch stress medium showed the highest 4.5 x107 chlamydospore production with highest chlamydospore production achieved after 78 hours, at pH 6 and 26˚C of temperature by using 1.5 x 107 inoculum size. Next, the spawn was pre-treated with 20% mixture of trehalose and skimmed milk lyoprotectant and lyophilized by using freeze dryer (Labconco) at -40ºC for 2 to 3 days. The spawn was sealed and stored at room temperature for 1 month. After that, the spawn was treated with 2 hours of rehydration treatment prior to inoculation on PDA medium. The chlamydospore-lyophilized spawn was then compared with the chlamydospore spawn which preserved using cryopreservation, sub-culturing, and 4˚C refrigeration techniques in aspect of growth rate and yield. Growth kinetic analysis found that chlamydospore spawn exhibited higher growth rate; 0.24d-1 compared to tissue culture spawn.
format	Thesis
title	Induction of chlamydospores in pleurotus pulmonarius (grey oyster mushroom) as inoculum and development of lyophilized spawn
title_short	Induction of chlamydospores in pleurotus pulmonarius (grey oyster mushroom) as inoculum and development of lyophilized spawn
title_full	Induction of chlamydospores in pleurotus pulmonarius (grey oyster mushroom) as inoculum and development of lyophilized spawn
title_fullStr	Induction of chlamydospores in pleurotus pulmonarius (grey oyster mushroom) as inoculum and development of lyophilized spawn
title_full_unstemmed	Induction of chlamydospores in pleurotus pulmonarius (grey oyster mushroom) as inoculum and development of lyophilized spawn
title_sort	induction of chlamydospores in pleurotus pulmonarius (grey oyster mushroom) as inoculum and development of lyophilized spawn
granting_institution	Universiti Malaysia Perlis (UniMAP)
granting_department	School of Bioprocess Engineering
url	http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77869/1/Page%201-24.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77869/2/Full%20text.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77869/4/Sharul%20Aida.pdf
_version_	1776104236565659648

Induction of chlamydospores in pleurotus pulmonarius (grey oyster mushroom) as inoculum and development of lyophilized spawn

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