Reduced graphene oxide-multi walled carbon nanotubes hybrid material as electrode for DNA biosensor

This thesis presents a novel thin film of reduced graphene oxide-multiwalled carbon nanotubes (rGO-MWCNTs) composites as a sensing film electrode for Deoxyribonucleic acid (DNA) immobilization and hybridization detection. This project consisted of three parts, which are the rGO-MWCNTs composite t...

Full description

Saved in:

Bibliographic Details
Format:	Thesis
Language:	English
Subjects:	Biosensors DNA Graphene Carbon nanotubes
Online Access:	http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77997/1/Page%201-24.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77997/2/Full%20text.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77997/4/Saeed%20Salem.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-unimap-77997
record_format	uketd_dc
spelling	my-unimap-779972023-03-06T04:10:18Z Reduced graphene oxide-multi walled carbon nanotubes hybrid material as electrode for DNA biosensor Ruslinda, A.Rahim, Dr. This thesis presents a novel thin film of reduced graphene oxide-multiwalled carbon nanotubes (rGO-MWCNTs) composites as a sensing film electrode for Deoxyribonucleic acid (DNA) immobilization and hybridization detection. This project consisted of three parts, which are the rGO-MWCNTs composite thin film preparation and characterization, the device fabrication processes description, and followed by the DNA immobilization and hybridization. In the first part, the thesis describes the graphene oxide preparation from graphite powder using improved Hummers’ method. Whereas, the multiwalled carbon nanotubes (MWCNTs) was functionalized through nitric acid oxidation process. Chemical reduction process was used to obtain the reduced graphene oxide using hydrazine as reduced agent. The MWCNTs, GO, and rGO-MWCNTs materials were mechanically sprayed on the silicon dioxide (SiO2) surface of the device channel using spray technique. Chitosan solution was mixed with the materials and sprayed on the device surface in order to increase the viscosity of the materials and strengthen their adhesion with the silicon dioxide surface by changing the surface characteristic from hydrophobic to hydrophilic. The morphology of the rGO-MWCNTs composite thin films were observed by field emission scanning electron microscope. The bonding of the rGOMWCNTs were examined using Fourier transform infrared spectroscopy. The phase structure of the materials were confirmed via X-ray powder diffraction. Secondly, the design, fabrication and evaluation of the device were descripted in details. In addition, the device fabrication processes contained of oxidation process for silicon dioxide layer growing, physical vapor deposition process which was used to deposit an aluminum layer on the silicon substrate to form the source and drain, mask designed, printed, and utilized in the pattern transfer process, and photolithography process which was carried out to create the channel of the device. The operation of the electrode is based on the surface charge adsorption of the film material interface. Finally, in the DNA immobilization and hybridization section where the novelty of the research introduced, the biosensor demonstrated high sensitivity to the complementary DNA target with a linear range from 500 pM to 100 pM. Furthermore, the biosensor demonstrated good selectivity, reproducibility, and long-term stability for DNA detection. The device has shown sufficient capability to distinguish between targets complementary DNA and different DNA sequences, such as non-complementary and single-mismatched DNA. The hybridization process of the non-complementary DNA has the smallest response (39 μA) due to the double standard DNA was not effectively formed. Whereas, the singlemismatched DNA has shown less response (55 μA) comparing with the complementary DNA (65 μA) due to the single mismatched base. The device accuracy was investigated and found to be 11.28 %. Since, the biosensor responded very well and demonstrated excellent detection capabilities, it is highly recommended to be used in detecting specific biomarkers and other targeted proteins. Universiti Malaysia Perlis (UniMAP) Thesis en http://dspace.unimap.edu.my:80/xmlui/handle/123456789/77997 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77997/3/license.txt 8a4605be74aa9ea9d79846c1fba20a33 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77997/1/Page%201-24.pdf 32a6307d96b031ea9c3c091982f06dc3 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77997/2/Full%20text.pdf a705f771c47343937c5de30bcc61152b http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77997/4/Saeed%20Salem.pdf 682fe8b0ea409967e10f64910e9f26a7 Universiti Malaysia Perlis (UniMAP) Biosensors DNA Graphene Carbon nanotubes Institute of Nano Electronic Engineering
institution	Universiti Malaysia Perlis
collection	UniMAP Institutional Repository
language	English
advisor	Ruslinda, A.Rahim, Dr.
topic	Biosensors DNA Graphene Carbon nanotubes
spellingShingle	Biosensors DNA Graphene Carbon nanotubes Reduced graphene oxide-multi walled carbon nanotubes hybrid material as electrode for DNA biosensor
description	This thesis presents a novel thin film of reduced graphene oxide-multiwalled carbon nanotubes (rGO-MWCNTs) composites as a sensing film electrode for Deoxyribonucleic acid (DNA) immobilization and hybridization detection. This project consisted of three parts, which are the rGO-MWCNTs composite thin film preparation and characterization, the device fabrication processes description, and followed by the DNA immobilization and hybridization. In the first part, the thesis describes the graphene oxide preparation from graphite powder using improved Hummers’ method. Whereas, the multiwalled carbon nanotubes (MWCNTs) was functionalized through nitric acid oxidation process. Chemical reduction process was used to obtain the reduced graphene oxide using hydrazine as reduced agent. The MWCNTs, GO, and rGO-MWCNTs materials were mechanically sprayed on the silicon dioxide (SiO2) surface of the device channel using spray technique. Chitosan solution was mixed with the materials and sprayed on the device surface in order to increase the viscosity of the materials and strengthen their adhesion with the silicon dioxide surface by changing the surface characteristic from hydrophobic to hydrophilic. The morphology of the rGO-MWCNTs composite thin films were observed by field emission scanning electron microscope. The bonding of the rGOMWCNTs were examined using Fourier transform infrared spectroscopy. The phase structure of the materials were confirmed via X-ray powder diffraction. Secondly, the design, fabrication and evaluation of the device were descripted in details. In addition, the device fabrication processes contained of oxidation process for silicon dioxide layer growing, physical vapor deposition process which was used to deposit an aluminum layer on the silicon substrate to form the source and drain, mask designed, printed, and utilized in the pattern transfer process, and photolithography process which was carried out to create the channel of the device. The operation of the electrode is based on the surface charge adsorption of the film material interface. Finally, in the DNA immobilization and hybridization section where the novelty of the research introduced, the biosensor demonstrated high sensitivity to the complementary DNA target with a linear range from 500 pM to 100 pM. Furthermore, the biosensor demonstrated good selectivity, reproducibility, and long-term stability for DNA detection. The device has shown sufficient capability to distinguish between targets complementary DNA and different DNA sequences, such as non-complementary and single-mismatched DNA. The hybridization process of the non-complementary DNA has the smallest response (39 μA) due to the double standard DNA was not effectively formed. Whereas, the singlemismatched DNA has shown less response (55 μA) comparing with the complementary DNA (65 μA) due to the single mismatched base. The device accuracy was investigated and found to be 11.28 %. Since, the biosensor responded very well and demonstrated excellent detection capabilities, it is highly recommended to be used in detecting specific biomarkers and other targeted proteins.
format	Thesis
title	Reduced graphene oxide-multi walled carbon nanotubes hybrid material as electrode for DNA biosensor
title_short	Reduced graphene oxide-multi walled carbon nanotubes hybrid material as electrode for DNA biosensor
title_full	Reduced graphene oxide-multi walled carbon nanotubes hybrid material as electrode for DNA biosensor
title_fullStr	Reduced graphene oxide-multi walled carbon nanotubes hybrid material as electrode for DNA biosensor
title_full_unstemmed	Reduced graphene oxide-multi walled carbon nanotubes hybrid material as electrode for DNA biosensor
title_sort	reduced graphene oxide-multi walled carbon nanotubes hybrid material as electrode for dna biosensor
granting_institution	Universiti Malaysia Perlis (UniMAP)
granting_department	Institute of Nano Electronic Engineering
url	http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77997/1/Page%201-24.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77997/2/Full%20text.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/77997/4/Saeed%20Salem.pdf
_version_	1776104258839511040

Reduced graphene oxide-multi walled carbon nanotubes hybrid material as electrode for DNA biosensor

Similar Items