Characterization of quartz crystal microbalance sensor for detection of bacteria inactivation after plasma treatment
This thesis presents a study of frequency response for detection of inactive bacteria using Quartz Crystal Microbalance sensor. The characteristics of frequency response towards detection of bacteria were discussed. The Escherichia coli bacteria with concentration x108 colony forming unit per mil...
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| Format: | Thesis |
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| Language: | English |
| Subjects: | |
| Online Access: | http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/78041/1/Page%201-24.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/78041/2/Full%20text.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/78041/3/Nor%20Aimi.pdf |
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| Summary: | This thesis presents a study of frequency response for detection of inactive bacteria
using Quartz Crystal Microbalance sensor. The characteristics of frequency response
towards detection of bacteria were discussed. The Escherichia coli bacteria with
concentration x108 colony forming unit per milliliter were inactivated using new
sterilization device which is atmospheric pressure plasma jet. A sensor device was
constructed to monitor frequency response of bacteria in different condition by using 9
MHz quartz crystal. For detecting the bacteria sample, the sensor surface was prepared
using Self-Assemble Monolayer technique together with Escherichia coli antibody as
bioreceptor for antigen recognition. Quartz Crystal Microbalance sensor with gold
electrodes were mounted in oscillator circuit and exposed to room temperature. The
Transistor-transistor Logic oscillator circuit was utilized since it was able to drive the
Quartz Crystal Microbalance sensor in air and liquid. Only one side of the crystal were
exposed to the liquid sample by using static measurement. The measured of frequency
response were observed in order to investigated the interfacial behavior of bacteria
Escherichia coli on surface of sensor. The results show that the bacteria after
inactivation have higher frequency shifting than bacteria before plasma treatment. The
difference in frequency responses showed that bacteria inactivated by atmospheric
plasma device have increment of mass. These situations are caused by several factors
such as unspecific molecule presence inside inactive bacteria sample, types of
bioreceptor, surface roughness and condition of electrode surface. The analysis also
includes piezoelectric effects, experimental conditions and the characteristic of surface
roughness. From average result of 10 sensors device utilized in the experiment, 8 were
successful in bacteria detection. The detection also depends on condition of electrode
surface. Moreover, this system has the ability to detect bacteria active and inactive in
liquid situation. |
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