Characterization of quartz crystal microbalance sensor for detection of bacteria inactivation after plasma treatment
This thesis presents a study of frequency response for detection of inactive bacteria using Quartz Crystal Microbalance sensor. The characteristics of frequency response towards detection of bacteria were discussed. The Escherichia coli bacteria with concentration x108 colony forming unit per mil...
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my-unimap-780412023-03-07T02:33:16Z Characterization of quartz crystal microbalance sensor for detection of bacteria inactivation after plasma treatment Siti Khadijah, Zaa'ba, Dr. This thesis presents a study of frequency response for detection of inactive bacteria using Quartz Crystal Microbalance sensor. The characteristics of frequency response towards detection of bacteria were discussed. The Escherichia coli bacteria with concentration x108 colony forming unit per milliliter were inactivated using new sterilization device which is atmospheric pressure plasma jet. A sensor device was constructed to monitor frequency response of bacteria in different condition by using 9 MHz quartz crystal. For detecting the bacteria sample, the sensor surface was prepared using Self-Assemble Monolayer technique together with Escherichia coli antibody as bioreceptor for antigen recognition. Quartz Crystal Microbalance sensor with gold electrodes were mounted in oscillator circuit and exposed to room temperature. The Transistor-transistor Logic oscillator circuit was utilized since it was able to drive the Quartz Crystal Microbalance sensor in air and liquid. Only one side of the crystal were exposed to the liquid sample by using static measurement. The measured of frequency response were observed in order to investigated the interfacial behavior of bacteria Escherichia coli on surface of sensor. The results show that the bacteria after inactivation have higher frequency shifting than bacteria before plasma treatment. The difference in frequency responses showed that bacteria inactivated by atmospheric plasma device have increment of mass. These situations are caused by several factors such as unspecific molecule presence inside inactive bacteria sample, types of bioreceptor, surface roughness and condition of electrode surface. The analysis also includes piezoelectric effects, experimental conditions and the characteristic of surface roughness. From average result of 10 sensors device utilized in the experiment, 8 were successful in bacteria detection. The detection also depends on condition of electrode surface. Moreover, this system has the ability to detect bacteria active and inactive in liquid situation. Universiti Malaysia Perlis (UniMAP) Thesis en http://dspace.unimap.edu.my:80/xmlui/handle/123456789/78041 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/78041/4/license.txt 8a4605be74aa9ea9d79846c1fba20a33 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/78041/1/Page%201-24.pdf 38bb21df263cf93f3bd9458ec38da125 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/78041/2/Full%20text.pdf a126629aca6e6528041ff97e7f690143 http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/78041/3/Nor%20Aimi.pdf d3026af97c91aa54a863b6f5e078a8c8 Universiti Malaysia Perlis (UniMAP) Quartz crystal microbalances Quartz crystals Piezoelectric devices Oscillators, Crystal Biosensors School of Mechatronic Engineering |
| institution |
Universiti Malaysia Perlis |
| collection |
UniMAP Institutional Repository |
| language |
English |
| advisor |
Siti Khadijah, Zaa'ba, Dr. |
| topic |
Quartz crystal microbalances Quartz crystals Piezoelectric devices Quartz crystal microbalances Biosensors |
| spellingShingle |
Quartz crystal microbalances Quartz crystals Piezoelectric devices Quartz crystal microbalances Biosensors Characterization of quartz crystal microbalance sensor for detection of bacteria inactivation after plasma treatment |
| description |
This thesis presents a study of frequency response for detection of inactive bacteria
using Quartz Crystal Microbalance sensor. The characteristics of frequency response
towards detection of bacteria were discussed. The Escherichia coli bacteria with
concentration x108 colony forming unit per milliliter were inactivated using new
sterilization device which is atmospheric pressure plasma jet. A sensor device was
constructed to monitor frequency response of bacteria in different condition by using 9
MHz quartz crystal. For detecting the bacteria sample, the sensor surface was prepared
using Self-Assemble Monolayer technique together with Escherichia coli antibody as
bioreceptor for antigen recognition. Quartz Crystal Microbalance sensor with gold
electrodes were mounted in oscillator circuit and exposed to room temperature. The
Transistor-transistor Logic oscillator circuit was utilized since it was able to drive the
Quartz Crystal Microbalance sensor in air and liquid. Only one side of the crystal were
exposed to the liquid sample by using static measurement. The measured of frequency
response were observed in order to investigated the interfacial behavior of bacteria
Escherichia coli on surface of sensor. The results show that the bacteria after
inactivation have higher frequency shifting than bacteria before plasma treatment. The
difference in frequency responses showed that bacteria inactivated by atmospheric
plasma device have increment of mass. These situations are caused by several factors
such as unspecific molecule presence inside inactive bacteria sample, types of
bioreceptor, surface roughness and condition of electrode surface. The analysis also
includes piezoelectric effects, experimental conditions and the characteristic of surface
roughness. From average result of 10 sensors device utilized in the experiment, 8 were
successful in bacteria detection. The detection also depends on condition of electrode
surface. Moreover, this system has the ability to detect bacteria active and inactive in
liquid situation. |
| format |
Thesis |
| title |
Characterization of quartz crystal microbalance sensor for detection of bacteria inactivation after plasma treatment |
| title_short |
Characterization of quartz crystal microbalance sensor for detection of bacteria inactivation after plasma treatment |
| title_full |
Characterization of quartz crystal microbalance sensor for detection of bacteria inactivation after plasma treatment |
| title_fullStr |
Characterization of quartz crystal microbalance sensor for detection of bacteria inactivation after plasma treatment |
| title_full_unstemmed |
Characterization of quartz crystal microbalance sensor for detection of bacteria inactivation after plasma treatment |
| title_sort |
characterization of quartz crystal microbalance sensor for detection of bacteria inactivation after plasma treatment |
| granting_institution |
Universiti Malaysia Perlis (UniMAP) |
| granting_department |
School of Mechatronic Engineering |
| url |
http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/78041/1/Page%201-24.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/78041/2/Full%20text.pdf http://dspace.unimap.edu.my:80/xmlui/bitstream/123456789/78041/3/Nor%20Aimi.pdf |
| _version_ |
1776104265281961984 |