Molecular characterization and antibiotic susceptibility profiles of bacteria associated with selected aquaculture farms in Sarawak

Disease threats and high demands of aquaculture products have forced aquaculturists to use antibiotics as a solution. However, misuse of antibiotics has led to the increase of antibiotic resistant bacteria population which threatens public health. This study aims to determine the extent of antibioti...

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Main Author:	Kathleen Michelle, Mikal
Format:	Thesis
Language:	English
Published:	2014
Subjects:	R Medicine (General)
Online Access:	http://ir.unimas.my/id/eprint/10920/1/Kathleen.pdf
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id	my-unimas-ir.10920
record_format	uketd_dc
spelling	my-unimas-ir.109202023-06-15T06:26:13Z Molecular characterization and antibiotic susceptibility profiles of bacteria associated with selected aquaculture farms in Sarawak 2014 Kathleen Michelle, Mikal R Medicine (General) SH Aquaculture. Fisheries. Angling Disease threats and high demands of aquaculture products have forced aquaculturists to use antibiotics as a solution. However, misuse of antibiotics has led to the increase of antibiotic resistant bacteria population which threatens public health. This study aims to determine the extent of antibiotic resistance and to detect the resistant genes in bacteria isolated from Sarawak aquaculture. Bacteria were isolated from five aquaculture farms in Sarawak (Borneo, Malaysia) and were analysed by (GTG)5-Polymerase Chain Reaction (PCR) for de-replication of clonal isolates. After dendrogram analysis, 94 selected isolates were identified using 16S rRNA PCR and DNA sequencing. Bacillus (38.3%) dominated the bacterial population followed by 16 other genera such as Staphylococcus and Acinetobacter. Susceptibility of all isolates towards 24 antibiotic discs was assessed using disk diffusion assay. Based on standard interpretative table by Clinical and Laboratory Standards Institute (CLSI), only streptomycin (85%) and ampicillin (56.8%) resulted more than 50% resistance. Multiple Antibiotic Resistant (MAR) index in this study ranged from 0.0 to 0.63. Isolates with MARI > 0.2 were recovered from high risk sources. PCR analysis revealed that 35% and 2% isolates possess streptomycin (strA-strB genes) and ampicillin (blaTEM genes) resistant genes, respectively. Resistance in other isolates might be expressed by genes which were not detected in this study. Findings in this study reveal low level of antibiotic resistance in Sarawak aquaculture bacteria except for the commonly used antibiotics in aquaculture sectors since decades ago. It is essential to continuously monitor the antibiotic resistant patterns to predict emerging MAR bacteria as well as preventing its widespread. Universiti Malaysia Sarawak, (UNIMAS) 2014 Thesis http://ir.unimas.my/id/eprint/10920/ http://ir.unimas.my/id/eprint/10920/1/Kathleen.pdf text en validuser masters Universiti Malaysia Sarawak, (UNIMAS) Faculty of Resources Science and Technology
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English
topic	R Medicine (General) R Medicine (General)
spellingShingle	R Medicine (General) R Medicine (General) Kathleen Michelle, Mikal Molecular characterization and antibiotic susceptibility profiles of bacteria associated with selected aquaculture farms in Sarawak
description	Disease threats and high demands of aquaculture products have forced aquaculturists to use antibiotics as a solution. However, misuse of antibiotics has led to the increase of antibiotic resistant bacteria population which threatens public health. This study aims to determine the extent of antibiotic resistance and to detect the resistant genes in bacteria isolated from Sarawak aquaculture. Bacteria were isolated from five aquaculture farms in Sarawak (Borneo, Malaysia) and were analysed by (GTG)5-Polymerase Chain Reaction (PCR) for de-replication of clonal isolates. After dendrogram analysis, 94 selected isolates were identified using 16S rRNA PCR and DNA sequencing. Bacillus (38.3%) dominated the bacterial population followed by 16 other genera such as Staphylococcus and Acinetobacter. Susceptibility of all isolates towards 24 antibiotic discs was assessed using disk diffusion assay. Based on standard interpretative table by Clinical and Laboratory Standards Institute (CLSI), only streptomycin (85%) and ampicillin (56.8%) resulted more than 50% resistance. Multiple Antibiotic Resistant (MAR) index in this study ranged from 0.0 to 0.63. Isolates with MARI > 0.2 were recovered from high risk sources. PCR analysis revealed that 35% and 2% isolates possess streptomycin (strA-strB genes) and ampicillin (blaTEM genes) resistant genes, respectively. Resistance in other isolates might be expressed by genes which were not detected in this study. Findings in this study reveal low level of antibiotic resistance in Sarawak aquaculture bacteria except for the commonly used antibiotics in aquaculture sectors since decades ago. It is essential to continuously monitor the antibiotic resistant patterns to predict emerging MAR bacteria as well as preventing its widespread.
format	Thesis
qualification_level	Master's degree
author	Kathleen Michelle, Mikal
author_facet	Kathleen Michelle, Mikal
author_sort	Kathleen Michelle, Mikal
title	Molecular characterization and antibiotic susceptibility profiles of bacteria associated with selected aquaculture farms in Sarawak
title_short	Molecular characterization and antibiotic susceptibility profiles of bacteria associated with selected aquaculture farms in Sarawak
title_full	Molecular characterization and antibiotic susceptibility profiles of bacteria associated with selected aquaculture farms in Sarawak
title_fullStr	Molecular characterization and antibiotic susceptibility profiles of bacteria associated with selected aquaculture farms in Sarawak
title_full_unstemmed	Molecular characterization and antibiotic susceptibility profiles of bacteria associated with selected aquaculture farms in Sarawak
title_sort	molecular characterization and antibiotic susceptibility profiles of bacteria associated with selected aquaculture farms in sarawak
granting_institution	Universiti Malaysia Sarawak, (UNIMAS)
granting_department	Faculty of Resources Science and Technology
publishDate	2014
url	http://ir.unimas.my/id/eprint/10920/1/Kathleen.pdf
_version_	1783728086533013504

Molecular characterization and antibiotic susceptibility profiles of bacteria associated with selected aquaculture farms in Sarawak

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