Development of single chain variable fragment antibodies against dengue virus

The heavy-chain and light-chain variable region genes of two anti-dengue hybridoma cell line (4G2 and MV8/1/B4-1) were isolated by polymerase chain reaction (PCR) and joined by a flexible peptide linker (Gly4Ser3), as a single-chain variable fragment (scFv) DNA gene. The scFv DNA gene were cloned in...

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Main Author:	Leong, Yee Wei.
Format:	Thesis
Language:	English
Published:	2006
Subjects:	RA0421 Public health Hygiene Preventive Medicine
Online Access:	http://ir.unimas.my/id/eprint/12719/3/Development%20of%20single%20chain%20variable%20fragment%20antibodies%20against%20dengue%20virus-cloning%2C%20expression%20and%20functional%20analysis%20%28fulltext%29.pdf
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id	my-unimas-ir.12719
record_format	uketd_dc
spelling	my-unimas-ir.127192023-03-07T08:40:15Z Development of single chain variable fragment antibodies against dengue virus 2006 Leong, Yee Wei. RA0421 Public health. Hygiene. Preventive Medicine The heavy-chain and light-chain variable region genes of two anti-dengue hybridoma cell line (4G2 and MV8/1/B4-1) were isolated by polymerase chain reaction (PCR) and joined by a flexible peptide linker (Gly4Ser3), as a single-chain variable fragment (scFv) DNA gene. The scFv DNA gene were cloned into a phagemid vector (pCANTAB 5 E) and expressed as a fusion protein with M13 phage in E.coli TG1. Affinity-selection was performed to select and enich scFv phages with strong binding to their respective antigens. Universiti Malaysia Sarawak, (UNIMAS) 2006 Thesis http://ir.unimas.my/id/eprint/12719/ http://ir.unimas.my/id/eprint/12719/3/Development%20of%20single%20chain%20variable%20fragment%20antibodies%20against%20dengue%20virus-cloning%2C%20expression%20and%20functional%20analysis%20%28fulltext%29.pdf text en validuser masters Institute of Health andCommunity Medicine Universiti Malaysia Sarawak, (UNIMAS)
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English
topic	RA0421 Public health Hygiene Preventive Medicine
spellingShingle	RA0421 Public health Hygiene Preventive Medicine Leong, Yee Wei. Development of single chain variable fragment antibodies against dengue virus
description	The heavy-chain and light-chain variable region genes of two anti-dengue hybridoma cell line (4G2 and MV8/1/B4-1) were isolated by polymerase chain reaction (PCR) and joined by a flexible peptide linker (Gly4Ser3), as a single-chain variable fragment (scFv) DNA gene. The scFv DNA gene were cloned into a phagemid vector (pCANTAB 5 E) and expressed as a fusion protein with M13 phage in E.coli TG1. Affinity-selection was performed to select and enich scFv phages with strong binding to their respective antigens.
format	Thesis
qualification_level	Master's degree
author	Leong, Yee Wei.
author_facet	Leong, Yee Wei.
author_sort	Leong, Yee Wei.
title	Development of single chain variable fragment antibodies against dengue virus
title_short	Development of single chain variable fragment antibodies against dengue virus
title_full	Development of single chain variable fragment antibodies against dengue virus
title_fullStr	Development of single chain variable fragment antibodies against dengue virus
title_full_unstemmed	Development of single chain variable fragment antibodies against dengue virus
title_sort	development of single chain variable fragment antibodies against dengue virus
granting_institution	Institute of Health andCommunity Medicine
granting_department	Universiti Malaysia Sarawak, (UNIMAS)
publishDate	2006
url	http://ir.unimas.my/id/eprint/12719/3/Development%20of%20single%20chain%20variable%20fragment%20antibodies%20against%20dengue%20virus-cloning%2C%20expression%20and%20functional%20analysis%20%28fulltext%29.pdf
_version_	1783728119816912896

Development of single chain variable fragment antibodies against dengue virus

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