Development of single chain variable fragment antibodies against dengue virus

The heavy-chain and light-chain variable region genes of two anti-dengue hybridoma cell line (4G2 and MV8/1/B4-1) were isolated by polymerase chain reaction (PCR) and joined by a flexible peptide linker (Gly4Ser3), as a single-chain variable fragment (scFv) DNA gene. The scFv DNA gene were cloned in...

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Bibliographic Details
Main Author: Leong, Yee Wei.
Format: Thesis
Language:English
Published: 2006
Subjects:
Online Access:http://ir.unimas.my/id/eprint/12719/3/Development%20of%20single%20chain%20variable%20fragment%20antibodies%20against%20dengue%20virus-cloning%2C%20expression%20and%20functional%20analysis%20%28fulltext%29.pdf
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