A study on interspecific hybridization between piper nigrum and piper colubrinum

ýInterspecific hybridization between P. nigrum L. and P. colubrinum Link. was attempted aiming to achieve Phytopthora foot rot resistant hybrid. P. nigrum, an important spice crop is tetraploid (2n=52) while P. colubrinum, a Phytopthora disease resistant species of Piper is diploid in nature. The ma...

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Main Author:	Chen, Yi Shang
Format:	Thesis
Language:	English
Published:	2011
Subjects:	SB Plant culture
Online Access:	http://ir.unimas.my/id/eprint/13757/1/Chen%20Yi%20Shang%20ft.pdf
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spelling	my-unimas-ir.137572023-05-09T06:38:47Z A study on interspecific hybridization between piper nigrum and piper colubrinum 2011 Chen, Yi Shang SB Plant culture ýInterspecific hybridization between P. nigrum L. and P. colubrinum Link. was attempted aiming to achieve Phytopthora foot rot resistant hybrid. P. nigrum, an important spice crop is tetraploid (2n=52) while P. colubrinum, a Phytopthora disease resistant species of Piper is diploid in nature. The main objective of this study is to find out the cross compatibility between the two species. Various experiments were conducted in this study i. e. reproductive biology study on parent plant, study on possible occurrence of apomixes in P. nigrum, artificial and natural pollination study and induction of tetraploid P. colubrinum. This study eventually proved that interspecific hybridization between P. nigrum L. and P. colubrinum Link) was incompatible by judging on negative result gathered from morphological and cytological studies on putative interspecific hybrid in first experiment of interspecific hybridization and zero success fruit set in second experiment of interspecific cross (after modified and improved methods of artificial pollination). There was also no germinati of P. nigrum in investigation of in vivo germination. Be interspecific hybridization, the studies were focused on developing a reliable method of artificial pollination between two species of Piper. Reproductive biology study on both parent plants i. e. P. nigrum (male) and P. colubrinum (female) enlightened that artificial pollination was ideally to be carried out approximately starting from 11 a. m until 5 p. m. In pollen viability study of P. colubrinum, anther dehiscence was discovered to occur around 9 a. m. and pollen was scientifically proved most viable at two hours after dehiscence and onward. Whilst, stigma receptivity study on P. nigrum suggested that stigma at stage I i. e. first emergence of stigmata and stage 3 i. e. complete emergence and wide spreading of stigmata were preferably to be selected for the hybridization. At the same time, apomixes was proved do not contribute to fruit set of P. nigrum. Besides reproductive biology study on both parent plants to assist artificial pollination, reliable methods of eliminating self pollination were also developed. A reliable method of eliminating self pollination can be achieved by sampled of single flower only per inflorescence together with bagging of inflorescence and physical emasculation. The applicability of these methods was proved practical. Eventually, chromosome doubling of P. colubrinum through colchicine was carried out as one of the possible solution for the cross incompatibility between P. nigrum and P. colubrinum. However, preliminary results showed the treatment was not successful. None of the colchicine treated plant (on shoot tip) showed positive results by judging on the cytological study. In vitro polyploidization that had been initiated also showed negative result. All the colchicine treated somatic embryos of P. colubrinum encounter blackening after the treatment. However, effort of inducing tetraploid P. colubrinum should be continued in future by using other antimitotic agent like oryzalin and trifluralin. Universiti Malaysia Sarawak, (UNIMAS) 2011 Thesis http://ir.unimas.my/id/eprint/13757/ http://ir.unimas.my/id/eprint/13757/1/Chen%20Yi%20Shang%20ft.pdf text en validuser masters Universiti Malaysia Sarawak, (UNIMAS) Faculty of Resource Science and Technology
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English
topic	SB Plant culture
spellingShingle	SB Plant culture Chen, Yi Shang A study on interspecific hybridization between piper nigrum and piper colubrinum
description	ýInterspecific hybridization between P. nigrum L. and P. colubrinum Link. was attempted aiming to achieve Phytopthora foot rot resistant hybrid. P. nigrum, an important spice crop is tetraploid (2n=52) while P. colubrinum, a Phytopthora disease resistant species of Piper is diploid in nature. The main objective of this study is to find out the cross compatibility between the two species. Various experiments were conducted in this study i. e. reproductive biology study on parent plant, study on possible occurrence of apomixes in P. nigrum, artificial and natural pollination study and induction of tetraploid P. colubrinum. This study eventually proved that interspecific hybridization between P. nigrum L. and P. colubrinum Link) was incompatible by judging on negative result gathered from morphological and cytological studies on putative interspecific hybrid in first experiment of interspecific hybridization and zero success fruit set in second experiment of interspecific cross (after modified and improved methods of artificial pollination). There was also no germinati of P. nigrum in investigation of in vivo germination. Be interspecific hybridization, the studies were focused on developing a reliable method of artificial pollination between two species of Piper. Reproductive biology study on both parent plants i. e. P. nigrum (male) and P. colubrinum (female) enlightened that artificial pollination was ideally to be carried out approximately starting from 11 a. m until 5 p. m. In pollen viability study of P. colubrinum, anther dehiscence was discovered to occur around 9 a. m. and pollen was scientifically proved most viable at two hours after dehiscence and onward. Whilst, stigma receptivity study on P. nigrum suggested that stigma at stage I i. e. first emergence of stigmata and stage 3 i. e. complete emergence and wide spreading of stigmata were preferably to be selected for the hybridization. At the same time, apomixes was proved do not contribute to fruit set of P. nigrum. Besides reproductive biology study on both parent plants to assist artificial pollination, reliable methods of eliminating self pollination were also developed. A reliable method of eliminating self pollination can be achieved by sampled of single flower only per inflorescence together with bagging of inflorescence and physical emasculation. The applicability of these methods was proved practical. Eventually, chromosome doubling of P. colubrinum through colchicine was carried out as one of the possible solution for the cross incompatibility between P. nigrum and P. colubrinum. However, preliminary results showed the treatment was not successful. None of the colchicine treated plant (on shoot tip) showed positive results by judging on the cytological study. In vitro polyploidization that had been initiated also showed negative result. All the colchicine treated somatic embryos of P. colubrinum encounter blackening after the treatment. However, effort of inducing tetraploid P. colubrinum should be continued in future by using other antimitotic agent like oryzalin and trifluralin.
format	Thesis
qualification_level	Master's degree
author	Chen, Yi Shang
author_facet	Chen, Yi Shang
author_sort	Chen, Yi Shang
title	A study on interspecific hybridization between piper nigrum and piper colubrinum
title_short	A study on interspecific hybridization between piper nigrum and piper colubrinum
title_full	A study on interspecific hybridization between piper nigrum and piper colubrinum
title_fullStr	A study on interspecific hybridization between piper nigrum and piper colubrinum
title_full_unstemmed	A study on interspecific hybridization between piper nigrum and piper colubrinum
title_sort	study on interspecific hybridization between piper nigrum and piper colubrinum
granting_institution	Universiti Malaysia Sarawak, (UNIMAS)
granting_department	Faculty of Resource Science and Technology
publishDate	2011
url	http://ir.unimas.my/id/eprint/13757/1/Chen%20Yi%20Shang%20ft.pdf
_version_	1783728139862540288

A study on interspecific hybridization between piper nigrum and piper colubrinum

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