Identification and analyses of genes associated with floral development in Sago palm (Metroxylon sagu)

Flowering process in plants is a complex system and involved various genes. Understandings of the role of genes involved in the flower development enable manipulation of aspects of the process in the future for crop improvement. In this study, efforts have been made to screen for CO and LFY. the gen...

Full description

Saved in:

Bibliographic Details
Main Author:	Syahrul Bariyah, Anji
Format:	Thesis
Language:	English
Published:	2010
Subjects:	SB Plant culture
Online Access:	http://ir.unimas.my/id/eprint/14240/1/Syahrul%20%28ft%29.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-unimas-ir.14240
record_format	uketd_dc
spelling	my-unimas-ir.142402023-08-08T04:18:15Z Identification and analyses of genes associated with floral development in Sago palm (Metroxylon sagu) 2010 Syahrul Bariyah, Anji SB Plant culture Flowering process in plants is a complex system and involved various genes. Understandings of the role of genes involved in the flower development enable manipulation of aspects of the process in the future for crop improvement. In this study, efforts have been made to screen for CO and LFY. the genes that have been known to be the genes that are activated very early in flowering process. As for the LFYand CO, a few fragments have been successfully isolated and sequenced. DDRT -PCR method was also undertaken in order to identifY the genes that are expressed in various sago palm tissues namely the meristem, inflorescence and leaf tissue) Using .I this technique, differentially expressed genes were isolated, sequenced and identified from the selected tissues. The expression of the successfully identified genes such as sugar transporter, chitinase, photosystem I psaH protein, caffeic acid 3-0methyltransferase, l-aminocyclopropane-l-carboxylic acid oxidase, mitogen activated protein kinase, universal stress protein, dynein light chain and 40S subunit ribosomal protein S21 possibly be involved in the flower development or have other functions. Partial sequences of chitinase obtained from DDRT-PCR work was employed as a probe to further amplifY the 3'-end and 5'-end regions of sago palm chitinase. Repeated amplification and sequences analysis confirmed that 3'end region of chitinase cDNA have been successfully amplified. However, the amplification of the complete sequences of the chitinase cDNA in the sago palm is not accomplished. A near complete chitinase cDNA isolated is 1143 bp long and contained 312 bp predicted amino acid. Alignment of nucleotide and amino acid have grouped this chitinase to the Domain Family 19 and Class I chitinase. Universiti Malaysia Sarawak (UNIMAS) 2010 Thesis http://ir.unimas.my/id/eprint/14240/ http://ir.unimas.my/id/eprint/14240/1/Syahrul%20%28ft%29.pdf text en validuser masters Universiti Malaysia Sarawak Faculty of Resource Science and Technology
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English
topic	SB Plant culture
spellingShingle	SB Plant culture Syahrul Bariyah, Anji Identification and analyses of genes associated with floral development in Sago palm (Metroxylon sagu)
description	Flowering process in plants is a complex system and involved various genes. Understandings of the role of genes involved in the flower development enable manipulation of aspects of the process in the future for crop improvement. In this study, efforts have been made to screen for CO and LFY. the genes that have been known to be the genes that are activated very early in flowering process. As for the LFYand CO, a few fragments have been successfully isolated and sequenced. DDRT -PCR method was also undertaken in order to identifY the genes that are expressed in various sago palm tissues namely the meristem, inflorescence and leaf tissue) Using .I this technique, differentially expressed genes were isolated, sequenced and identified from the selected tissues. The expression of the successfully identified genes such as sugar transporter, chitinase, photosystem I psaH protein, caffeic acid 3-0methyltransferase, l-aminocyclopropane-l-carboxylic acid oxidase, mitogen activated protein kinase, universal stress protein, dynein light chain and 40S subunit ribosomal protein S21 possibly be involved in the flower development or have other functions. Partial sequences of chitinase obtained from DDRT-PCR work was employed as a probe to further amplifY the 3'-end and 5'-end regions of sago palm chitinase. Repeated amplification and sequences analysis confirmed that 3'end region of chitinase cDNA have been successfully amplified. However, the amplification of the complete sequences of the chitinase cDNA in the sago palm is not accomplished. A near complete chitinase cDNA isolated is 1143 bp long and contained 312 bp predicted amino acid. Alignment of nucleotide and amino acid have grouped this chitinase to the Domain Family 19 and Class I chitinase.
format	Thesis
qualification_level	Master's degree
author	Syahrul Bariyah, Anji
author_facet	Syahrul Bariyah, Anji
author_sort	Syahrul Bariyah, Anji
title	Identification and analyses of genes associated with floral development in Sago palm (Metroxylon sagu)
title_short	Identification and analyses of genes associated with floral development in Sago palm (Metroxylon sagu)
title_full	Identification and analyses of genes associated with floral development in Sago palm (Metroxylon sagu)
title_fullStr	Identification and analyses of genes associated with floral development in Sago palm (Metroxylon sagu)
title_full_unstemmed	Identification and analyses of genes associated with floral development in Sago palm (Metroxylon sagu)
title_sort	identification and analyses of genes associated with floral development in sago palm (metroxylon sagu)
granting_institution	Universiti Malaysia Sarawak
granting_department	Faculty of Resource Science and Technology
publishDate	2010
url	http://ir.unimas.my/id/eprint/14240/1/Syahrul%20%28ft%29.pdf
_version_	1783728153351421952

Identification and analyses of genes associated with floral development in Sago palm (Metroxylon sagu)

Similar Items